Effects of temperature and wavelength choice on in-situ dissolution test of Cimetidine tablets

AbstractThe effects of temperature and wavelength choice on in-situ dissolution test instrument of Cimetidine were studied. Absorbance (A)<1.0 is required when using a fiber-optic dissolution test system. The detection wavelength of λmax (218nm) was replaced by 244nm to carry out this test. The absorbance of Cimetidine solution at different temperature showed an obvious change. Calibration of Cimetidine solution should be tested at the same temperature (37°C) with the test solution. A suitable wavelength with smaller tangent slope could be chosen for in-situ dissolution test of Cimetidine tablets

The preliminary strategies about the innovation of curriculum system of forensic medicine

法医学是应用医学、生物学、化学和其他自然科学理论和技术解决与法律有关的医学问题的一门应用学科。课程体系是指同一专业不同课程门类按照门类顺序排列，是教学内容和进程的总和，一门学科需要不断的调整和完善课程体系，才能适应学科的发展。本文从国内法医学课程设置的现状分析、国外法医学课程体系简况介绍、我国法医学课程体系改革和创新的必要性等几方面对我国法医学专业课程设置的改革进行讨论，提出可行的法医学专业课程体系改革措施和策略，与同行探讨，为法医学专业课程体系的改革提供借鉴。Forensic medicine is an applied science which solved the legal issues concerning with the medical science and utilized the theories and the technologies of medicine, biology, chemistry and other natural sciences. Curriculum system is the summation of teaching contents and teaching proceeding, including all courses of a discipline and arranged with a specific order. The curriculum system of a discipline requires adjustment and innovation to suit the developing circumstance. The present paper will firstly analyze the current status of curriculum system of forensic medicine in China; briefly introduce the oversea curriculum system of forensic medicine; and necessity of innovation of forensic curriculum system in China. Then give some reformatory strategies of China’s curriculum system of forensic medicine

Open Design and 3D Printing of Face Shields: The Case Study of a UK-China Initiative

At the start of the COVID-19 outbreak, many countries lacked personal protective equipment (PPE) to protect healthcare workers. To address this problem, open design and 3D printing technologies were adopted to provide much-in-need PPEs for key workers. This paper reports an initiative by designers and engineers in the UK and China. The case study approach and content analysis method were used to study the stakeholders, the design process, and other relevant issues such as regulation. Good practice and lessons were summarised, and suggestions for using distributed 3D printing to supply PPEs were made. It concludes that 3D printing has played an important role in producing PPEs when there was a shortage of supply, and distributed manufacturing has the potential to quickly respond to local small-bench production needs. In the future, clearer specification, better match of demands and supply, and quicker evaluation against relevant regulations will provide efficiency and quality assurance for 3D printed PPE supplies

Cigarette Smoke Extract Stimulates Rat Pulmonary Artery Smooth Muscle Cell Proliferation via PKC-PDGFB Signaling

Accumulating evidence suggests a direct role for cigarette smoke in pulmonary vascular remodeling, which contributes to the development of pulmonary hypertension. However, the molecular mechanisms underlying this process remain poorly understood. Platelet-derived growth factor (PDGF) is a potential mitogen and chemoattractant implicated in several biological processes, including cell survival, proliferation, and migration. In this study, we investigated the effect of cigarette smoke extract (CSE) on cell proliferation of rat pulmonary artery smooth muscle cells (rPASMCs). We found that stimulation of rPASMCs with CSE significantly increased cell proliferation and promoted cell cycle progression from G1 phase to the S and G2 phases. CSE treatment also significantly upregulated the mRNA and protein levels of PDGFB and PDGFRβ. Our study also revealed that Rottlerin, an inhibitor of PKCδ signaling, prevented CSE-induced cell proliferation, attenuated the increase of S and G2 phase populations induced by CSE treatment, and downregulated PDGFB and PDGFRβ mRNA and protein levels in rPASMCs exposed to CSE. Collectively, our data demonstrated that CSE-induced cell proliferation of rPASMCs involved upregulation of the PKCδ-PDGFB pathway

4-Carb­oxy-2-methyl-1H-imidazol-3-ium-5-carboxyl­ate monohydrate

In the title compound, C6H6N2O4·H2O, one carboxyl group is deprotonated and one imidazole N atom is protonated. The organic mol­ecule, excluding methyl H atoms, is essentially planar, with an r.m.s. deviation of 0.0156 (1) Å. In the crystal structure, inter­molecular N—H⋯O hydrogen bonds link mol­ecules into chains along the b axis; these chains are further linked via O—H⋯O hydrogen bonds involving the water O atoms and carboxyl O atoms, generating a two-dimensional supra­molecular framework

The Clinical Signifcance of Expression of ERCC1 and PKCalpha in Non-small Cell Lung Cancer

Background and objective Excision repair cross-complementing 1 (Excision-Repair Cross-Complementing 1, ERCC1), an important member of the DNA repair gene family, plays a key role in nucleotide excision repair and apoptosis of tumor cells. Protein kinase C-α (Protein kinase C, PKCα), an isozyme in protein kinase C family, is an important signaling molecule in signal transduction pathways of tumors, which has been implicated in malignant transformation and proliferation. The aim of this study was to explore the clinical significance of ERCC1 and PKCα in non-small cell lung cancer (NSCLC). Methods The expression of ERCC1 and PKCα were examined by immunohistochemistry (IHC) in the specimens of 51 cases of NSCLC patients tissue and 21 cases of paracancerous tissue. The relationship between detected data and patients′ clinical parameters was analyzed by SPSS 13.0 software. Results The positive expression rate of ERCC1 and PKCα in NSCLC tissues was significantly higher than paracancerous tissues (Ρ<0.05). Expression of ERCC1 was closely related to clinical stage and N stage. The positive rate of ERCC1 was higher in III+IV or N1+N2 stage patients compared with I+II or N0 stage (Ρ=0.011, P=0.015). We also found that 5-year survival of negative group of ERCC1 was remarkably higher than that of positive group by χ2 test (Ρ<0.05). Expression of ERCC1 was positively correlative to PKCα by Spearman′s correlation analysis (r=0.425, P=0.002) in NSCLC. Conclusion The results suggest ERCC1 and PKCα might be correlated with the development of NSCLC. ERCC1 might be related to prognosis of NSCLC. There might be existed a mechanism of coordination or regulation between ERCC1 and PKCα