Primitive roles for inhibitory interneurons in developing frog spinal cord

Understanding the neuronal networks in the mammal spinal cord is hampered by the diversity of neurons and their connections. The simpler networks in developing lower vertebrates may offer insights into basic organization. To investigate the function of spinal inhibitory interneurons in Xenopus tadpoles, paired whole-cell recordings were used. We show directly that one class of interneuron, with distinctive anatomy, produces glycinergic, negative feedback inhibition that can limit firing in motoneurons and interneurons of the central pattern generator during swimming. These same neurons also produce inhibitory gating of sensory pathways during swimming. This discovery raises the possibility that some classes of interneuron, with distinct functions later in development, may differentiate from an earlier class in which these functions are shared. Preliminary evidence suggests that these inhibitory interneurons express the transcription factor engrailed, supporting a probable homology with interneurons in developing zebrafish that also express engrailed and have very similar anatomy and functions

Anti-Fall: A Non-intrusive and Real-time Fall Detector Leveraging CSI from Commodity WiFi Devices

Fall is one of the major health threats and obstacles to independent living for elders, timely and reliable fall detection is crucial for mitigating the effects of falls. In this paper, leveraging the fine-grained Channel State Information (CSI) and multi-antenna setting in commodity WiFi devices, we design and implement a real-time, non-intrusive, and low-cost indoor fall detector, called Anti-Fall. For the first time, the CSI phase difference over two antennas is identified as the salient feature to reliably segment the fall and fall-like activities, both phase and amplitude information of CSI is then exploited to accurately separate the fall from other fall-like activities. Experimental results in two indoor scenarios demonstrate that Anti-Fall consistently outperforms the state-of-the-art approach WiFall, with 10% higher detection rate and 10% less false alarm rate on average.Comment: 13 pages,8 figures,corrected version, ICOST conferenc

The Theological Virtue of Faith as the Foundation of the Christian Moral Life

Contents Acknowledgements List of Abbreviations Chapters Introduction Chapter One: Scriptural Notion of Faith and the Life of Faith Introduction Faith as God’s Work of Salvation in Jesus Christ The Gospel as the Content of Faith Faith and the “Obedience of Faith” are Gifts of God’s Grace Faith Transforms the Life of the Believer The Life of Faith is “Faith Working Through Love” Conclusion Chapter Two: The Moral Theology of Thomas Aquinas and Faith in Christ Introduction Happiness as the Keystone of the Moral Life Faith and Man’s Perfection and Happiness “Living Faith” and “Faith Working Through Love” Faith and the “New Law” The New Law and “Faith Working Through Love” Conclusion Chapter Three: Faith and the Moral Life in Post-Vatican II Ecclesial Documents Introduction Jesus Christ is the Center of the Moral Life Faith in Christ and the Moral Life “Faith Working Through Love” as the Summit of the Moral Life Conclusion Conclusion Sources Cite

VIENA2: A Driving Anticipation Dataset

Action anticipation is critical in scenarios where one needs to react before the action is finalized. This is, for instance, the case in automated driving, where a car needs to, e.g., avoid hitting pedestrians and respect traffic lights. While solutions have been proposed to tackle subsets of the driving anticipation tasks, by making use of diverse, task-specific sensors, there is no single dataset or framework that addresses them all in a consistent manner. In this paper, we therefore introduce a new, large-scale dataset, called VIENA2, covering 5 generic driving scenarios, with a total of 25 distinct action classes. It contains more than 15K full HD, 5s long videos acquired in various driving conditions, weathers, daytimes and environments, complemented with a common and realistic set of sensor measurements. This amounts to more than 2.25M frames, each annotated with an action label, corresponding to 600 samples per action class. We discuss our data acquisition strategy and the statistics of our dataset, and benchmark state-of-the-art action anticipation techniques, including a new multi-modal LSTM architecture with an effective loss function for action anticipation in driving scenarios.Comment: Accepted in ACCV 201

Classifier ensemble for uncertain data stream classification

Currently available algorithms for data stream classification are all designed to handle precise data, while data with uncertainty or imperfection is quite natural and widely seen in real-life applications. Uncertainty can arise in attribute values as well as in class values. In this paper, we focus on the classification of streaming data that has different degrees of uncertainty within class values. We propose two types of ensemble based algorithms, Static Classifier Ensemble (SCE) and Dynamic Classifier Ensemble (DCE) for mining uncertain data streams. Experiments on both synthetic and real-life data set are made to compare and contrast our proposed algorithms. The experimental results reveal that DCE algorithm outperforms SCE algorithm

Melatonin reduced volume of cerebral infarct induced by photothrombosis in wild-type mice, not in Cyclooxygenase-1 gene knockout mice

Cyclooxygenase (COX) is crucial in inflammation and plays important role in cerebral ischemia. Anti-inflammatory effects of melatonin have been verified in previous studies. In this study, cerebral blood flow (CBF) was monitored during operation, infarct volume (IFV) was determined with 5-triphenyltetrazolium chloride (TTC) staining and MR image, and neurological functions were evaluated with turn in an alley and fall pole test in both COX-1-gene knockout and wide-type mice with or without melatonin administration 3 days after photothrombosis. CBF reduction, IFV and neurological deficits were not significantly different in COX-1 wild-type and COX-1 knockout mice. Melatonin (15 mg/kg) intraperitoneal injection decreased the CBF reduction, IFV and the latency to turn in an alley in COX-1 wide-type mice, whereas the neuroprotective effect of melatonin was attenuated in COX-1 knockout mice. We concluded that melatonin reduced susceptibility to photothrombotic stroke. COX-1 gene knockout does not alter the susceptibility to cerebral ischemia caused by photothrombosis. COX-1 plays an important role in the pathway of the protection of melatonin.published_or_final_versio

Correction: Lone pair driven anisotropy in antimony chalcogenide semiconductors

Correction for 'Lone pair driven anisotropy in antimony chalcogenide semiconductors' by Xinwei Wang et al., Phys. Chem. Chem. Phys., 2022, 24, 7195-7202, https://doi.org/10.1039/D1CP05373F

Identification of novel clostridium perfringens type E strains that carry an iota toxin plasmid with a functional enterotoxin gene

Clostridium perfringens enterotoxin (CPE) is a major virulence factor for human gastrointestinal diseases, such as food poisoning and antibiotic associated diarrhea. The CPE-encoding gene (cpe) can be chromosomal or plasmid-borne. Recent development of conventional PCR cpe-genotyping assays makes it possible to identify cpe location (chromosomal or plasmid) in type A isolates. Initial studies for developing cpe genotyping assays indicated that all cpe-positive strains isolated from sickened patients were typable by cpe-genotypes, but surveys of C. perfringens environmental strains or strains from feces of healthy people suggested that this assay might not be useful for some cpe-carrying type A isolates. In the current study, a pulsed-field gel electrophoresis Southern blot assay showed that four cpe-genotype untypable isolates carried their cpe gene on a plasmid of ~65 kb. Complete sequence analysis of the ~65 kb variant cpe-carrying plasmid revealed no intact IS elements and a disrupted cytosine methyltransferase (dcm) gene. More importantly, this plasmid contains a conjugative transfer region, a variant cpe gene and variant iota toxin genes. The toxin genes encoded by this plasmid are expressed based upon the results of RT-PCR assays. The ~65 kb plasmid is closely related to the pCPF4969 cpe plasmid of type A isolates. MLST analyses indicated these isolates belong to a unique cluster of C. perfringens. Overall, these isolates carrying a variant functional cpe gene and iota toxin genes represent unique type E strains. © 2011 Miyamoto et al

Berkovich Nanoindentation on AlN Thin Films

Berkovich nanoindentation-induced mechanical deformation mechanisms of AlN thin films have been investigated by using atomic force microscopy (AFM) and cross-sectional transmission electron microscopy (XTEM) techniques. AlN thin films are deposited on the metal-organic chemical-vapor deposition (MOCVD) derived Si-doped (2 × 1017 cm−3) GaN template by using the helicon sputtering system. The XTEM samples were prepared by means of focused ion beam (FIB) milling to accurately position the cross-section of the nanoindented area. The hardness and Young’s modulus of AlN thin films were measured by a Berkovich nanoindenter operated with the continuous contact stiffness measurements (CSM) option. The obtained values of the hardness and Young’s modulus are 22 and 332 GPa, respectively. The XTEM images taken in the vicinity regions just underneath the indenter tip revealed that the multiple “pop-ins” observed in the load–displacement curve during loading are due primarily to the activities of dislocation nucleation and propagation. The absence of discontinuities in the unloading segments of load–displacement curve suggests that no pressure-induced phase transition was involved. Results obtained in this study may also have technological implications for estimating possible mechanical damages induced by the fabrication processes of making the AlN-based devices

Evaluation of Phage Display Discovered Peptides as Ligands for Prostate-Specific Membrane Antigen (PSMA)

The aim of this study was to identify potential ligands of PSMA suitable for further development as novel PSMA-targeted peptides using phage display technology. The human PSMA protein was immobilized as a target followed by incubation with a 15-mer phage display random peptide library. After one round of prescreening and two rounds of screening, high-stringency screening at the third round of panning was performed to identify the highest affinity binders. Phages which had a specific binding activity to PSMA in human prostate cancer cells were isolated and the DNA corresponding to the 15-mers were sequenced to provide three consensus sequences: GDHSPFT, SHFSVGS and EVPRLSLLAVFL as well as other sequences that did not display consensus. Two of the peptide sequences deduced from DNA sequencing of binding phages, SHSFSVGSGDHSPFT and GRFLTGGTGRLLRIS were labeled with 5-carboxyfluorescein and shown to bind and co-internalize with PSMA on human prostate cancer cells by fluorescence microscopy. The high stringency requirements yielded peptides with affinities KD∼1 μM or greater which are suitable starting points for affinity maturation. While these values were less than anticipated, the high stringency did yield peptide sequences that apparently bound to different surfaces on PSMA. These peptide sequences could be the basis for further development of peptides for prostate cancer tumor imaging and therapy. © 2013 Shen et al