18,482 research outputs found
Integrative analyses of transcriptome sequencing identify novel functional lncRNAs in esophageal squamous cell carcinoma.
Long non-coding RNAs (lncRNAs) have a critical role in cancer initiation and progression, and thus may mediate oncogenic or tumor suppressing effects, as well as be a new class of cancer therapeutic targets. We performed high-throughput sequencing of RNA (RNA-seq) to investigate the expression level of lncRNAs and protein-coding genes in 30 esophageal samples, comprised of 15 esophageal squamous cell carcinoma (ESCC) samples and their 15 paired non-tumor tissues. We further developed an integrative bioinformatics method, denoted URW-LPE, to identify key functional lncRNAs that regulate expression of downstream protein-coding genes in ESCC. A number of known onco-lncRNA and many putative novel ones were effectively identified by URW-LPE. Importantly, we identified lncRNA625 as a novel regulator of ESCC cell proliferation, invasion and migration. ESCC patients with high lncRNA625 expression had significantly shorter survival time than those with low expression. LncRNA625 also showed specific prognostic value for patients with metastatic ESCC. Finally, we identified E1A-binding protein p300 (EP300) as a downstream executor of lncRNA625-induced transcriptional responses. These findings establish a catalog of novel cancer-associated functional lncRNAs, which will promote our understanding of lncRNA-mediated regulation in this malignancy
A Unified Approach to the Classical Statistical Analysis of Small Signals
We give a classical confidence belt construction which unifies the treatment
of upper confidence limits for null results and two-sided confidence intervals
for non-null results. The unified treatment solves a problem (apparently not
previously recognized) that the choice of upper limit or two-sided intervals
leads to intervals which are not confidence intervals if the choice is based on
the data. We apply the construction to two related problems which have recently
been a battle-ground between classical and Bayesian statistics: Poisson
processes with background, and Gaussian errors with a bounded physical region.
In contrast with the usual classical construction for upper limits, our
construction avoids unphysical confidence intervals. In contrast with some
popular Bayesian intervals, our intervals eliminate conservatism (frequentist
coverage greater than the stated confidence) in the Gaussian case and reduce it
to a level dictated by discreteness in the Poisson case. We generalize the
method in order to apply it to analysis of experiments searching for neutrino
oscillations. We show that this technique both gives correct coverage and is
powerful, while other classical techniques that have been used by neutrino
oscillation search experiments fail one or both of these criteria.Comment: 40 pages, 15 figures. Changes 15-Dec-99 to agree more closely with
published version. A few small changes, plus the two substantive changes we
made in proof back in 1998: 1) The definition of "sensitivity" in Sec. V(C).
It was inconsistent with our actual definition in Sec. VI. 2) "Note added in
proof" at end of the Conclusio
Measurement of the branching fractions of psi(2S) -> 3(pi+pi-) and J/psi -> 2(pi+pi-)
Using data samples collected at sqrt(s) = 3.686GeV and 3.650GeV by the BESII
detector at the BEPC, the branching fraction of psi(2S) -> 3(pi+pi-) is
measured to be [4.83 +- 0.38(stat) +- 0.69(syst)] x 10^-4, and the relative
branching fraction of J/psi -> 2(pi+pi-) to that of J/psi -> mu+mu- is measured
to be [5.86 +- 0.19(stat) +- 0.39(syst)]% via psi(2S) -> (pi+pi-)J/psi, J/psi
-> 2(pi+pi-). The electromagnetic form factor of 3(pi+pi-) is determined to be
0.21 +- 0.02 and 0.20 +- 0.01 at sqrt(s) = 3.686GeV and 3.650GeV, respectively.Comment: 17pages, 7 figures, submitted to Phys. Rev.
Observation of p pbar pi^0 and p pbar eta in psi' decays
The processes psi'-->p pbar pi^0 and psi'-->p pbar eta are studied using a
sample of 14 million psi' decays collected with the Beijing Spectrometer at the
Beijing Electron-Positron Collider. The branching fraction of psi'-->p pbar
pi^0 is measured with improved precision as (13.2\pm 1.0\pm 1.5)\times 10^{-5},
and psi'-->p pbar eta is observed for the first time with a branching fraction
of (5.8\pm 1.1\pm 0.7)\times 10^{-5}, where the first errors are statistical
and the second ones are systematic.Comment: 15 pages, 8 figures and 3 table
Partial wave analysis of J/psi to p pbar pi0
Using a sample of 58 million events collected with the BESII
detector at the BEPC, more than 100,000 events are
selected, and a detailed partial wave analysis is performed. The branching
fraction is determined to be . A long-sought `missing' , first observed in , is observed in this decay too, with mass and width of
MeV/c and MeV/c,
respectively. Its spin-parity favors . The masses, widths, and
spin-parities of other states are obtained as well.Comment: Add one author nam
Measurements of J/psi decays into phi pi^0, phi eta, and phi eta^prime
Based on 5.8x10^7 J/psi events detected in BESII, the branching fractions of
J/psi--> phi eta and phi eta^prime are measured for different eta and eta^prime
decay modes. The results are significantly higher than previous measurements.
An upper limit on B(J/psi--> phi pi^0) is also obtained.Comment: 9 pages, 10 figure
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