Friable Callus Induction of Hedera nepalensis var. sinensis

[Objectives] The purpose was to establish an induction system for friable callus of Hedera nepalensis var. sinensis with different parts. [Methods] By screening the most suitable explant and adjusting the hormone ratio of medium, friable calli of H. nepalensis var. sinensis were induced. [Results] The calli could be induced from leaves, petioles and stem segments, but the ideal explant was stem segments, with induction rate reaching 98%. The optimal medium for callus proliferation was MS + 0.5 mg/L KT + 1.0 mg/L 2,4-D + 30.0 g/L sucrose. After 3-4 generations of subculture on MS+0.5 mg/L BA+1.0 mg/L 2,4-D+30.0 g/L sucrose, favorable friable calli of H. nepalensis var. sinensis were obtained. [Conclusions] The friable calli induced in this experiment can lay a foundation for in-vitro regeneration and cellular secondary metabolite production of H. nepalensis var. sinensis