Doctor of Philosophy

dissertationAutophagy is a catabolic pathway that degrades damaged proteins and organelles in lysosome; however, the exact role of autophagy in cardiac function in physiological and pathological states is incompletely understood. To investigate the role of autophag

Effect of a combination of infrared irradiation and magnesium sulfate wet compress on infection and healing of episiotomy incision in puerperae

Purpose: To investigate the effect of a combination of infrared irradiation and magnesium sulfate wet compress on infection and healing of episiotomy incision in puerperae during spontaneous delivery. Methods: A total of 120 puerperae who underwent lateral episiotomy in Jinan Maternity and Child Hospital Affiliated to Shandong First Medical University from January 2019 to January 2020 were used as study subjects. They were randomly assigned to group A (n = 60) and group B (n = 60). Group B received external application of anerdian, while group A was treated with infrared irradiation and magnesium sulfate wet compress, in addition to receiving the treatment given to group B. The two groups were compared with respect to perineal edema, levels of inflammatory factors, wound pain grading, degree of incision healing, incision healing time, and incidence of infection. Results: Group A patients had significantly lighter perineal edema and more pronounced pain relief than group B patients (p < 0.05). The number of puerperae with grade A healing and grade C healing in group A was significantly higher than that in group B (p < 0.05). Incision healing time and incidence of infection were lower in group A than in group B (p < 0.05). Conclusion: The combination of infrared irradiation and magnesium sulfate wet compress effectively mitigates perineal edema in puerperae, reduces pain, enhances the healing of incision, and lowers maternal infection. Thus, this combination strategy may have some merit in clinical practice

Review on the progress for physical simulation for gas reservoirs co-production in multi-pressure system

The gas reservoirs co-production in multi-pressure system is one of the important measures to improve the development efficiency of the superposed gas-bearing systems. However, the co-production effect is not ideal due to the special reservoir forming background. The mechanism of co-production and high-efficient development of the multi-pressure system has become an key scientific problem, which restricts the efficient exploration and development of superposed gas-bearing systems. This paper focuses on the gas reservoirs co-production in multi-pressure system, and divides the physical simulation types of co-production into two separate fields: coalbed methane and non coalbed methane. It clarifies the current research status of gas reservoirs co-production in multi-pressure system from the aspects of device functions and characteristics, understanding of co-production, and existing problems. Firstly, the large-scale physical simulation test device can effectively eliminate or weaken the problems of homogeneous single-type reservoir samples, single monitoring data means and single stress loading form caused by paralleling multiple core grippers to build the physical simulation model. The development direction of the physical simulation for co-production in multi-pressure system should be to achieve true three-dimensional heterogeneous complex in-situ stress state of large-scale heterogeneous multi-type reservoir samples. The characteristics of fluid pressure transmission between adjacent reservoirs, the inter-layer crossflow, the multi-phase natural gas symbiosis should be considered. On this basis, the sensitivity of co-production of multi-pressure system to reservoir physical properties was deeply summarized. The differences in inter-layer pressure difference, permeability, effective stress, water saturation and other factors may induce the fluid interference and reservoir gas production damage, and optimizing co-production style may be a way to reduce the fluid interference and reservoir gas production damage. In totally, the next research should focus on exploring the influence of the coupling effect of low porosity and low permeability, gas water two-phase flow, multiphase gas symbiosis and coexistence of multiple types of reservoirs on the dynamic evolution law of reservoir-wellbore flow field induced by co-production fluid interference, clarifying the reservoir damage and its mechanism of different phase fluid intrusions on the reservoir, and revealing the coupling flow characteristics of inter-layer crossflow and wellbore pipe flow considering the fluid interference effect

Clinical characteristics and cognitive function in bipolar disorder patients with different onset symptom

BackgroundIn recent years, studies on the clinical features and cognitive impairment of patients with different first-episode types of bipolar disorder have received increasing attention. The patients with bipolar disorder may present with different symptoms at first onset. The aim of this study is to assess the cognitive functions of a patient’s index episode of bipolar disorder, depression or mania, on risk factors of effecting on cognitive functions.MethodOne hundred sixty eight patients with bipolar disorder diagnosed for the first time were enrolled in the study. All patients were divided into two groups according to their index episode of bipolar disorder, either depression or mania. Seventy three patients of the cohort had an index episode mania and 95 patients had initial symptoms of depression. Demographic and clinical disease characteristic data of all enrolled patients were collected. Meanwhile, 75 healthy controls were included. Demographic data of controls were collected. The cognitive functions of all patients and controls were detected by continuous performance test (CPT), digital span test (DST) and Wisconsin card sorting test (WCST). The main cognitive functions data were compared among the mania group, depression group and control group. The relevant risk factors affecting cognitive function were analyzed.Results(1) Most patients with bipolar disorder had an index episode depression (56.55% vs. 43.45%). Compared with the depression group, the mania group had later age of onset [(24.01 ± 4.254) vs. (22.25 ± 6.472), t = 2. 122, p = 0.035]. The education level of patient groups was lower than control group (p < 0.001). (2) The healthy control group’s DST, WCST and CPT scores were better than the patient groups (All p < 0.05). The mania group’s DST (forward, reverse, sum), WCST (total responses, completed classifications, correct responses, incorrect responses, percentage of correct responses, completed the number of responses required for classification, the percentage of conceptualization level, the number of persistent responses, non-persistent errors), CPT (2 digit score, 3 digit score, 4 digit score) was better than the depression group (p < 0.05). (3) In mania group, correlation analysis showed that all CPT parameter, inverse digit span, and the sum of DST was negatively correlated with the education level (All p < 0.05). The CPT-4 digit score was negatively correlated with onset age (p < 0.05). In the WCST, the number of correct responses, the percentage of correct responses and the percentage of conceptualization level were positively correlated with the BRMS score (All p < 0.05). The number of false responses and persistent responses were negatively correlated with the BRMS score (All p < 0.05). The number of persistent errors and percentage of persistent errors was positively correlated with education years (All p < 0.05). In depression group, there was a positive correlation between inverse digit span and the education level (p < 0.05).ConclusionIn our study, there were cognitive impairments in attention, memory, and executive function of patients with different onset syndromes of bipolar disorder. Compared with the mania group, the degree of cognitive impairments in bipolar patients with the depressive episode was more severe. The risk factors affecting cognitive impairments included the age of onset, education level, number of hospitalizations and severity of illness

Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm

Background: Human pluripotent stem cells offer the best available model to study the underlying cellular and molecular mechanisms of human embryonic lineage specification. However, it is not fully understood how individual stem cells exit the pluripotent state and transition towards their respective progenitor states. Results: Here, we analyze the transcriptomes of human embryonic stem cell-derived lineage-specific progenitors by single-cell RNA-sequencing (scRNA-seq). We identify a definitive endoderm (DE) transcriptomic signature that leads us to pinpoint a critical time window when DE differentiation is enhanced by hypoxia. The molecular mechanisms governing the emergence of DE are further examined by time course scRNA-seq experiments, employing two new statistical tools to identify stage-specific genes over time (SCPattern) and to reconstruct the differentiation trajectory from the pluripotent state through mesendoderm to DE (Wave-Crest). Importantly, presumptive DE cells can be detected during the transitory phase from Brachyury (T)+ mesendoderm toward a CXCR4+ DE state. Novel regulators are identified within this time window and are functionally validated on a screening platform with a T-2A-EGFP knock-in reporter engineered by CRISPR/Cas9. Through loss-of-function and gain-of-function experiments, we demonstrate that KLF8 plays a pivotal role modulating mesendoderm to DE differentiation. Conclusions: We report the analysis of 1776 cells by scRNA-seq covering distinct human embryonic stem cell-derived progenitor states. By reconstructing a differentiation trajectory at single-cell resolution, novel regulators of the mesendoderm transition to DE are elucidated and validated. Our strategy of combining single-cell analysis and genetic approaches can be applied to uncover novel regulators governing cell fate decisions in a variety of systems. Electronic supplementary material The online version of this article (doi:10.1186/s13059-016-1033-x) contains supplementary material, which is available to authorized users

Robust Formation Control against Observation Losses

Distributed formation control has received increasing attention in multiagent systems. Maintaining certain geometry in space is advantageous in many applications such as space interferometry and underwater sensing. At present, there is a variety of distributed solutions for agents to converge to desired formations and track a series of prescribed maneuvers. They typically rely on the relative kinematics e.g., relative positions of the neighboring agents as state observations for the local controller. In harsh working environments, the acquisition of the relative kinematics is challenged and observation losses might occur, which can be detrimental to the optimality of formation. In this work, observation losses in noisy environments are addressed under a distributed formation control framework. Three types of solutions are proposed to enhance the robustness which is evaluated through the improvements of tracking error, convergence speed, and smoothness of trajectories in both random and permanent loss settings. Firstly, a relative localization technique is proposed using formation itself as a spatial constraint. Secondly, a dynamic model is established for the agents entailed by a Kalman filter-based solution. Finally, a fusion of the previous two types is inspired and it exhibits superior performance than both aforementioned types individually. This work not only provides means of relative localization without additional sensor data but also shares insights into coping with random or permanent graph changes for stress-based formation control systems. This could potentially lead to the exploration of formation control with subgraphs or energy-efficient sensing as future directions.Electrical Engineering | Circuits and System