Engineering Porous Poly(lactic acid) Scaffolds with High Mechanical Performance via a Solid State Extrusion/Porogen Leaching Approach

A knotty issue concerning the poor mechanical properties exists in the porogen leaching approach to porous scaffolds, despite its advantage in tuning pore structure. To address this hurdle, solid state extrusion (SSE) combined with porogen leaching was utilized to engineer porous scaffolds of poly(lactic acid) (PLA). Advances introduced by poly(ethylene glycol) (PEG) caused the PLA ductile to be processed and, on the other hand, enabled the formation of interconnected pores. Thus, a well-interconnected porous architecture with high connectivity exceeding 97% and elevated porosity over 60% was obtained in the as-prepared PLA scaffolds with the composition of NaCl higher than 75.00 wt % and PEG beyond 1.25 wt %. More strikingly, the pore walls of macropores encompassed countless micropores and rough surface topography, in favor of transporting nutrients and metabolites as well as cell attachment. The prominent compressive modulus of the PLA scaffolds was in the range of 85.7–207.4 MPa, matching the normal modulus of human trabecular bone (50–250 MPa). By means of alkaline modification to improve hydrophilicity, biocompatible porous PLA scaffolds exhibited good cell attachment. These results suggest that the SSE/porogen leaching approach provides an eligible clue for fabricating porous scaffolds with high mechanical performance for use as artificial extracellular matrices

Screening the Key Region of Sunlight Regulating the Flavonoid Profiles of Young Shoots in Tea Plants (<i>Camellia sinensis</i> L.) Based on a Field Experiment

Both UV and blue light have been reported to regulate the biosynthesis of flavonoids in tea plants; however, the respective contributions of the corresponding regions of sunlight are unclear. Additionally, different tea cultivars may respond differently to altered light conditions. We investigated the responses of different cultivars (‘Longjing 43’, ‘Zhongming 192’, ‘Wanghai 1’, ‘Jingning 1’ and ‘Zhonghuang 2’) to the shade treatments (black and colored nets) regarding the biosynthesis of flavonoids. For all cultivars, flavonol glycosides showed higher sensitivity to light conditions compared with catechins. The levels of total flavonol glycosides in the young shoots of different tea cultivars decreased with the shade percentages of polyethylene nets increasing from 70% to 95%. Myricetin glycosides and quercetin glycosides were more sensitive to light conditions than kaempferol glycosides. The principal component analysis (PCA) result indicated that shade treatment greatly impacted the profiles of flavonoids in different tea samples based on the cultivar characteristics. UV is the crucial region of sunlight enhancing flavonol glycoside biosynthesis in tea shoots, which is also slight impacted by light quality according to the results of the weighted correlation network analysis (WGCNA). This study clarified the contributions of different wavelength regions of sunlight in a field experiment, providing a potential direction for slightly bitter and astringent tea cultivar breeding and instructive guidance for practical field production of premium teas based on light regimes

Development of Genome-Wide DNA Polymorphism Database for Map-Based Cloning of Rice Genes

DNA polymorphism is the basis to develop molecular markers that are widely used in genetic mapping today. A genome-wide rice (Oryza sativa) DNA polymorphism database has been constructed in this work using the genomes of Nipponbare, a cultivar of japonica, and 93-11, a cultivar of indica. This database contains 1,703,176 single nucleotide polymorphisms (SNPs) and 479,406 Insertion/Deletions (InDels), approximately one SNP every 268 bp and one InDel every 953 bp in rice genome. Both SNPs and InDels in the database were experimentally validated. Of 109 randomly selected SNPs, 107 SNPs (98.2%) are accurate. PCR analysis indicated that 90% (97 of 108) of InDels in the database could be used as molecular markers, and 68% to 89% of the 97 InDel markers have polymorphisms between other indica cultivars (Guang-lu-ai 4 and Long-te-pu B) and japonica cultivars (Zhong-hua 11 and 9522). This suggests that this database can be used not only for Nipponbare and 93-11, but also for other japonica and indica cultivars. While validating InDel polymorphisms in the database, a set of InDel markers with each chromosome 3 to 5 marker was developed. These markers are inexpensive and easy to use, and can be used for any combination of japonica and indica cultivars used in this work. This rice DNA polymorphism database will be a valuable resource and important tool for map-based cloning of rice gene, as well as in other various research on rice (http://shenghuan.shnu.edu.cn/ricemarker)

Additional file 2: Figure S1. of Malaria control along China-Myanmar Border during 2007–2013: an integrated impact evaluation

Map of project location relative to neighboring countries. Five special regions in Myanmar = Kachin Special Region I (KSR1), Kachin Special Region II (KSR2), Kokang, Shan Special Region II (Wa) and Shan Special Region IV (SR4). 12 counties of Round 6 in China (black dots) = Tengchong(TC), longchuan(LC), Yingjiang(YJ), Lianghe (LH), Longling(LL), Zhenkang(ZK), Gengma(GM),Cangyuan(CY), Ximeng(XM), Menglian(ML), Lancang(LC) and Menghai(MH). Seven counties of Round 10 in China (red dots) = Gongshan(GS), Fugong(FG), Lushui(LS), Ruili(RL), Luxi, Jinghong(JH) and Mengla(MLa). Table S1. Parasite prevalence in five special regions of Myanmar from 2008 to 2013. Table S2. Annual parasite incidence (API/10,000) based on project reports in five special regions of Myanmar from 2008 to 2013. Table S3. Annual parasite incidence (API/10, 000) and number of imported malaria cases based on China information system for disease control and prevention (CISDCP) in 19 counties, China from 2006 to 2013. (DOC 472 kb

Additional file 1: of Malaria control along China-Myanmar Border during 2007–2013: an integrated impact evaluation

Multilingual abstracts in the six official working languages of the United Nations. (PDF 757 kb

Parallelizing Industrial Hard Real-Time Applications for the parMERASA Multicore

International audienceThe EC project parMERASA (Multicore Execution of Parallelized Hard Real-Time Applications Supporting Analyzability) investigated timing-analyzable parallel hard real-time applications running on a predictable multicore processor. A pattern-supported parallelization approach was developed to ease sequential to parallel program transformation based on parallel design patterns that are timing analyzable. The parallelization approach was applied to parallelize the following industrial hard real-time programs: 3D path planning and stereo navigation algorithms (Honeywell International s.r.o.), control algorithm for a dynamic compaction machine (BAUER Maschinen GmbH), and a diesel engine management system (DENSO AUTOMOTIVE Deutschland GmbH). This article focuses on the parallelization approach, experiences during parallelization with the applications, and quantitative results reached by simulation, by static WCET analysis with the OTAWA tool, and by measurement-based WCET analysis with the RapiTime tool