レーザークラッド法により作製した308Lステンレス鋼の特性評価

広島大学(Hiroshima University)博士(工学)Doctor of Engineeringdoctora

Dark Septate Endophytes Isolated From Wild Licorice Roots Grown in the Desert Regions of Northwest China Enhance the Growth of Host Plants Under Water Deficit Stress

This study aimed to explore dark septate endophytes (DSE) that may improve the cultivation of medicinal plants in arid ecosystems. We isolated and identified eight DSE species (Acremonium nepalense, Acrocalymma vagum, Alternaria chartarum, Alternaria chlamydospora, Alternaria longissima, Darksidea alpha, Paraphoma chrysanthemicola, and Preussia terricola) colonizing the roots of wild licorice (Glycyrrhiza uralensis) in the desert areas of northwest China. Moreover, we investigated the osmotic stress tolerance of the DSE using pure culture, along with the performance of licorice plants inoculated with the DSE under drought stress in a growth chamber, respectively. Here, five species were first reported in desert habitats. The osmotic-stress tolerance of DSE species was highly variable, A. chlamydospora and P. terricola increased the total biomass and root biomass of the host plant. All DSE except A. vagum and P. chrysanthemicola increased the glycyrrhizic acid content; all DSE except A. chartarum increased the glycyrrhizin content under drought stress. DSE × watering regimen improved the glycyrrhizic acid content, soil organic matter, and available nitrogen. Structural equation model analysis showed that DSE × watering regimen positively affected soil organic matter, and total biomass, root length, glycyrrhizic acid, and glycyrrhizin (Shapotou site); and positively affected soil organic matter, available phosphorus, and glycyrrhizin (Minqin site); and positively affected the root length (Anxi site). DSE from the Shapotou site accounted for 8.0, 13.0, and 11.3% of the variations in total biomass, root biomass, and active ingredient content; DSE from the Minqin site accounted for 6.6 and 8.3% of the variations in total biomass and root biomass; DSE from the Anxi site accounted for 4.2 and 10.7% of the variations in total biomass and root biomass. DSE × watering regimen displayed a general synergistic effect on plant growth and active ingredient contents. These findings suggested that the DSE–plant interactions were affected by both DSE species and DSE originating habitats. As A. chlamydospora and P. terricola positively affected the total biomass, root biomass, and active ingredient content of host plants under drought stress, they may have important uses as promoters for the cultivation of licorice in dryland agriculture

An efficient optimal design methodology for abnormal noise control of automobile transmission in the neutral idle condition

The modal analysis of the automobile transmission housing assembly is formulated using 3D graphics software Pro/E together with Finite Element Method in order to reduce the noise and vibration by accurately identifying the noise source of the transmission in the neutral idle condition. Besides, the calculation of meshing frequency of gear pair is conducted. After comparing model analysis results with the calculation results, we can intuitively analysis the dynamic characteristics of the transmission housing assembly and find out sensitive parts of the housing vibration. In order to enhance the structural stiffness of the transmission housing assembly and allow intentionally the natural frequency of the transmission to avoid the gear meshing frequency, we increase the thickness of thin plate or add the strengthening rib on the weak parts of the transmission housing assembly. The results of the above modified modal reanalysis show that low mode frequencies of the transmission housing assembly are greatly improved after optimization, and avoid the gear meshing excitation frequency effectively. The probability of resonance for the transmission housing assembly in use will be greatly reduced, which can well achieve the purpose of enhancing the dynamic characteristics of the transmission housing assembly

Optimization Model of Transit Signal Priority Control for Intersection and Downstream Bus Stop

Transit signal priority has a positive effect on improving traffic condition and level of transit service in the urban area. In this paper, a passenger-based transit signal priority (TSP) optimization model is formulated to optimize intersection signal phasing based on minimizing accessibility-based passenger delay at the intersection and increased waiting-delay at the downstream bus stop simultaneously. Genetic Algorithm is utilized to calculate passenger-based optimization model that is calibrated by evening rush hour actual traffic data (17:30–18:30, October 13th–October 15th, 2015) along Shuiximen Boulevard in Nanjing, China. The performance of the proposed optimization model in decreasing delay and improving system reliability is simulated and evaluated by VISSIM-based simulation platform, and the results illustrate that the proposed optimization model presents promising outcomes in decreasing accessibility-based passenger delay at intersection (average reduction of 12%) and passenger waiting-delay at downstream bus service stop (average reduction of 18%) compared with traditional vehicle-based TSP optimization method in rush hour

Climatic change controls productivity variation in global grasslands.

Detection and identification of the impacts of climate change on ecosystems have been core issues in climate change research in recent years. In this study, we compared average annual values of the normalized difference vegetation index (NDVI) with theoretical net primary productivity (NPP) values based on temperature and precipitation to determine the effect of historic climate change on global grassland productivity from 1982 to 2011. Comparison of trends in actual productivity (NDVI) with climate-induced potential productivity showed that the trends in average productivity in nearly 40% of global grassland areas have been significantly affected by climate change. The contribution of climate change to variability in grassland productivity was 15.2-71.2% during 1982-2011. Climate change contributed significantly to long-term trends in grassland productivity mainly in North America, central Eurasia, central Africa, and Oceania; these regions will be more sensitive to future climate change impacts. The impacts of climate change on variability in grassland productivity were greater in the Western Hemisphere than the Eastern Hemisphere. Confirmation of the observed trends requires long-term controlled experiments and multi-model ensembles to reduce uncertainties and explain mechanisms

Identification and characterization of the expression profile of microRNAs in Anopheles anthropophagus

BACKGROUND: Anopheles anthropophagus, one of the most important mosquito-borne disease vectors in Asia, mainly takes blood meals from humans and transmits both malaria and filariae. MicroRNAs (miRNAs) are small non-coding RNAs, and play a critical role in many cellular processes, including development, differentiation, apoptosis and innate immunity. METHODS: We investigated the global miRNA expression profile of male and female adults of A. anthropophagus using illumina Hiseq2000 sequencing combined with Northern blot. RESULTS: By using the miRNAs of the closely-related species Anopheles gambiae and Aedes aegypti as reference, we obtained 102 miRNAs candidates out of 12.43 million raw sequencing reads for male and 16.51 million reads for female, with 81 of them found as known miRNAs in An. gambiae and/or Ae. aegypti, and the remaining 21 miRNAs were considered as novel. By analyzing the revised read count of miRNAs in male and female, 29 known miRNAs show sexual difference expression: >2-fold in the read count of the same miRNAs in male and female. Especially for miR-989, which is highly expressed in the female mosquitoes, but shows almost no detected expression in male mosquitoes, indicating that miR-989 may be involved in the physiological activity of female mosquito adults. The expression of four miRNAs in different growth stages of mosquito were further identified by Northern blot. Several miRNAs show the stage-specific expression, of which miR-2943 only expressed in the egg stage, suggesting that miR-2943 may be associated with the development of mosquito eggs. CONCLUSIONS: The present study represents the first global characterization of An. anthropophagus miRNAs in sexual differences and stage-specific functions. A better understanding of the functions of these miRNAs will offer new insights in mosquito biology and has implications for the effective control of mosquito-borne infectious diseases