Increased Expression and Altered Methylation of HERVWE1 in the Human Placentas of Smaller Fetuses from Monozygotic, Dichorionic, Discordant Twins

<div><h3>Background</h3><p>The human endogenous retroviral family W, Env(C7), member 1 gene (<em>HERVWE1</em>) is thought to participate in trophoblast cell fusion, and its expression is diminished in the placentas of singleton intrauterine growth-retarded pregnancies. However, there is limited information about the role of <em>HERVWE1</em> in discordant fetal growth in twins. This study was to compare <em>HERVWE1</em> gene expression between the placentas of discordant monozygotic twins and to identify its regulation by methylation.</p> <h3>Methodology/Principal Findings</h3><p>Fetuses from twenty-one pairs of monozygotic, dichorionic, discordant twins were marked as “smaller” or “larger” according to birth weight. Placental <em>HERVWE1</em> mRNA and protein expression profiles were analyzed using quantitative RT-PCR and immunohistochemistry (IHC) staining. Methylation profiles of the <em>HERVWE1</em> promoter region were analyzed using a pyrosequencing assay. DNA methyltransferase (<em>DNMT</em>) transcript levels were analyzed by RT-PCR. 5-methyl cytosine (5-MC) was stained using an immunohistochemical assay. There was a significant negative correlation between <em>HERVWE1</em> mRNA levels and birth weight in twins (<em>P</em><0.01). Whereas the mean methylation level of the <em>HERVWE1</em> promoter region was diminished in the smaller group in discordant twins(<em>P</em><0.01), increased mRNA and protein levels of <em>HERVWE1</em> were found in smaller fetuses compared with larger fetuses in discordant twins(<em>P</em><0.01). There was no significant difference in 5-MC staining intensity between discordant twins (<em>P</em>>0.05). The <em>DNMT3b3</em> mRNA levels in the smaller group were significantly downregulated compared with the larger group in discordant twins(<em>P</em><0.05), whereas the <em>DNMT3b7</em> mRNA levels in the smaller group were significantly upregulated compared with the larger group in discordant twins(<em>P</em><0.05).</p> <h3>Conclusions/Significance</h3><p>In discordant, monozygotic, dichorionic twins, <em>HERVWE1</em> expression was higher in smaller fetuses and lower in larger fetuses. Methylation of the <em>HERVWE1</em> gene promoter region may participate in the regulation of <em>HERVWE1</em> gene expression in discordant twin pregnancies.</p> </div

Mood disorders influencing endometriosis and adenomyosis: Mendelian randomisation study

Background Many studies have found an association between mood-disorder-related traits and endometriosis and adenomyosis. However, the cause–effect relationship remains unclear. Aims We conducted Mendelian randomisation analyses to evaluate any causal relationship between mood disorders and endometriosis as well as different sites of endometriosis. Method Summary-level statistics for mood-disorder-related traits and endometriosis (8288 cases, 68 969 controls) in European populations were derived from large-scale data-sets of genome-wide association studies. A two-sample Mendelian randomisation was performed using the inverse-variance weighted and weight median methods. Further sensitivity analyses, including heterogeneity, pleiotropy and leave-one-out analyses, were conducted to test the consistency of the results. Results Genetically determined mood swings (odds ratio = 2.557, 95% CI: 1.192–5.483, P = 0.016) and major depression (odds ratio = 1.233, 95% CI: 1.019–1.493, P = 0.031) were causally associated with an increased risk of endometriosis. Mood swings (odds ratio = 4.238, 95% CI: 1.194–15.048, P = 0.025) and major depression (odds ratio = 1.512, 95% CI: 1.052–2.173, P = 0.025) were also causally associated with the risk of adenomyosis. Sensitivity analyses confirmed the reliability of the results. Conclusions Our results suggest that mood-disorder-related traits increase the risk of endometriosis and adenomyosis. This study provides new insights into the potential pathogenesis of endometriosis and adenomyosis, and highlights the importance of preventing endometriosis and adenomyosis in patients with mood-disorder-related traits

Efficiency, equity, and Adequacy: Financial Reform of China’s Compulsory Education

Danke Li is a contributing author, “家庭教育决策在中国农村教育社会性别不平等中的含义” (Household Decisions on Education and Its Implications for Gender Inequality in Rural China), pp. 131-149 (in Chinese).https://digitalcommons.fairfield.edu/history-books/1054/thumbnail.jp

Acoustic Emission Waveform Analysis of Sandstone Failure with Different Water Content

Previous studies have shown that water can reduce the acoustic emission (AE) energy and other parameters during rock failure. However, the fracture mechanism of rock can be better reflected by analyzing the AE waveform. Therefore, this paper conducted experiments of uniaxial compression on sandstone samples of various water contents and collected AE signals simultaneously. Analyses of fast Fourier transform (FFT) and Hilbert-Huang transform (HHT) were performed on the AE waveform when the sample failed. The results show that as the water content increases, the frequency and intensity of the AE signal will decrease. The influence of water on the intensity of the AE signal is greater than that on the frequency. Through the analysis of the energy mechanism of rock failure, it is pointed out that the frequency and intensity of AE signal are closely related to elastic energy index WET and burst energy index KE. The research results have guiding significance for the monitoring of rockburst

Numerical simulation study on shear resistance of anchorage joints considering tensile–shear fracture criterion of 2G-NPR bolt

Abstract 2G-NPR bolt (the 2nd generation Negative Poisson’s Ratio bolt) is a new type of bolt with high strength, high toughness and no yield platform. It has significant effects on improving the shear strength of jointed rock mass and controlling the stability of surrounding rock. To achieve an accurate simulation of bolted joint shear tests, we have studied a numerical simulation method that takes into account the 2G-NPR bolt's tensile–shear fracture criterion. Firstly, the indoor experimental study on the tensile–shear mechanical properties of 2G-NPR bolt is carried out to explore its mechanical properties under different tensile–shear angles, and the fracture criterion of 2G-NPR bolt considering the tensile–shear angle is established. Then, a three-dimensional numerical simulation method considering the tensile–shear mechanical constitutive and fracture criterion of 2G-NPR bolt, the elastoplastic mechanical behavior of surrounding rock and the damage and deterioration of grouting body is proposed. The feasibility and accuracy of the method are verified by comparing with the indoor shear test results of 2G-NPR bolt anchorage joints. Finally, based on the numerical simulation results, the deformation and stress of the bolt, the distribution of the plastic zone of the rock mass, the stress distribution and the damage of the grouting body are analyzed in detail. The research results can provide a good reference value for the practical engineering application and shear mechanical performance analysis of 2G-NPR bolt

Identification of Crucial lncRNAs, miRNAs, mRNAs, and Potential Therapeutic Compounds for Polycystic Ovary Syndrome by Bioinformatics Analysis

Background. This study was aimed at mining crucial long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) for the development of polycystic ovary syndrome (PCOS) based on the coexpression and the competitive endogenous RNA (ceRNA) theories and investigating the underlying therapeutic drugs that may function by reversing the expression of lncRNAs, miRNAs, and mRNAs. Methods. RNA (GSE106724, GSE114419, GSE137684, and GSE138518) or miRNA (GSE84376 and GSE138572) expression profile datasets of PCOS patients were downloaded from the Gene Expression Omnibus database. The weighted gene coexpression network analysis (WGCNA) using four RNA datasets was conducted to construct the lncRNA-mRNA coexpression networks, while the common differentially expressed miRNAs in two miRNA datasets and module RNAs were used to establish the ceRNA network. A protein-protein interaction (PPI) network was created to explore the potential interactions between genes. Gene Ontology and KEGG pathway enrichment analyses were performed to explore the functions of genes in networks. Connectivity Map (CMap) and Comparative Toxicogenomics Database (CTD) analyses were performed to identify potential therapeutic agents for PCOS. Results. Three modules (black, magenta, and yellow) were identified to be PCOS-related after WGCNA analysis, in which KLF3-AS1-PLCG2, MAPKAPK5-AS1-MAP3K14, and WWC2-AS2-TXNIP were important coexpression relationship pairs. WWC2-AS2-hsa-miR-382-PLCG2 was a crucial ceRNA loop in the ceRNA network. The PPI network showed that MAP3K14 and TXNIP could interact with hub genes PLK1 (degree=21) and TLR1 (degree=18), respectively. These genes were enriched into mitosis (PLK1), immune response (PLCG2 and TLR1), and cell cycle (TXNIP and PLK1) biological processes. Ten small molecule drugs (especially quercetin) were considered to be therapeutical for PCOS. Conclusion. Our study may provide a novel insight into the mechanisms and therapy for PCOS

Real-Time Experimental Monitoring for Water Absorption Evolution Behaviors of Sandstone in Mogao Grottoes, China

Rock mass has typical pore structure, and the induced coupling effects of fluid and solid matrix appear in the disaster evolution process of deep energy exploitation and overground rock hydration. As a representative case, influenced by the water absorption environment, the surrounding rock and murals of Mogao Grottoes produce hydration diseases, which may be related to unclear interaction mechanisms between the surrounding rock and water. In this study, the self-developed physical experimental system for real-time experimental monitoring was applied to test the water absorption evolution behaviors of sandstone. The experimental results showed that the water evaporation of the rock sample during the process of water absorption could be measured through this well-designed physical experimental system, and the actual water absorption of the rock sample is the difference between the decrease of water in the water storage bucket, measured by the balance and the water evaporation in the process of experiment; by drawing the actual water absorption curve of the rock sample, the time when the water absorption of the rock sample reaches saturation could be determined accurately; and the curve of water absorption with time could be expressed as an exponential function. The experimental techniques and methods in this study provide a feasible research idea for studying the water absorption evolution behaviors and mechanisms of the surrounding rock weathering when it meets water, and have significance for revealing the disease mechanisms of the surrounding sandstone in Mogao Grottoes, China