Examining Cell Movements in the Neurulating Chick Embryo

The avian embryo is a popular animal model because it is widely available (Antin et al., 2004), it is easily manipulated, and it can provide important insights into normal and abnormal embryo development (Kulesa, 2004). While in vivo and in vitro cultures of chick embryos are common, in ovo cultures are rarer, and none have been designed where the egg did not have to be resealed afterwards. The present study aimed to develop a set-up in which the egg would be windowed without resealing the egg so that the embryo would remain accessible for experimental manipulation. As well, this study aimed to track cell movement during neurulation by microinjecting points of dye along the embryo. Two prototypes were developed based on the concept that temperature and moisture controlled air passing over the windowed egg would serve as a blanket. When these prototypes were unable to keep the embryo alive, a protocol developed by Kulesa and Fraser (2004) was adapted for the study. This protocol involved the construction of a Teflon window which was placed in the windowed egg and sealed with beeswax. Initial microinjection tests with Fast Green FCF showed that the dye dissipated quickly after injection, most likely because of the hydrophilicity of the dye. Therefore, a list of non-fluorescent, hydrophobic dyes were chosen and tested for suitability to cell tracking. Time restrictions prevented the actual cell tracking experiments from taking place, but it was found that Oil Red O fulfilled the criteria. As Oil Red O is usually used to identify lipids in static experiments, it remains to be seen whether it would function as a vital dye. Future experiments include expanding the set-up for use with a confocal microscope for a 4-D rendering of cell movement, and taking advantage of the symmetrical nature of neurulation in the chick embryo to examine perturbations to the normal progress of development, via drugs such as valproic acid

e-Leadership through strategic alignment: an empirical study of small- and medium-sized enterprises in the digital age

Small- and medium-sized enterprises (SMEs) play an important role in the European economy. A critical challenge faced by SME leaders, as a consequence of the continuing digital technology revolution, is how to optimally align business strategy with digital technology to fully leverage the potential offered by these technologies in pursuit of longevity and growth. There is a paucity of empirical research examining how e-leadership in SMEs drives successful alignment between business strategy and digital technology fostering longevity and growth. To address this gap, in this paper we develop an empirically derived e-leadership model. Initially we develop a theoretical model of e-leadership drawing on strategic alignment theory. This provides a theoretical foundation on how SMEs can harness digital technology in support of their business strategy enabling sustainable growth. An in-depth empirical study was undertaken interviewing 42 successful European SME leaders to validate, advance and substantiate our theoretically driven model. The outcome of the two stage process – inductive development of a theoretically driven e-leadership model and deductive advancement to develop a complete model through in-depth interviews with successful European SME leaders – is an e-leadership model with specific constructs fostering effective strategic alignment. The resulting diagnostic model enables SME decision makers to exercise effective e-leadership by creating productive alignment between business strategy and digital technology improving longevity and growth prospects

Main Streets and Green Spaces: Creating a Community Vision in Indian Orchard

Main Street and Green Spaces takes a focused look at the neighborhood of Indian Orchard and provides strategies for urban design and landscape architecture to support residents’ visions for the future. With the Studio’s partnership between the Indian Orchard Citizen’s Council, Grow IO (Wellspring Harvest), and the Springfield Office of Planning and Economic Development, the overarching goal for each design team was to create a cohesive, phased plan for redeveloping Main Street around the Oak and Main intersection and Parker and the Ludlow Bridge. Special attention was requested for exploring traffic calming measures and ways to support the local businesses present

The cultural roots of compositional capability in China: balanced moderation

A large number of Chinese firms lack the resources for having competitive advantages. Under this severe constraint, such firms are forced to find new paths toward developing certain competitive advantages, including the ability to combine ordinary resources into novel competitive advantages, which is referred to as compositional capability. Such a special capability underlying novel competitive advantages is related to certain cultural factors, such as the Chinese cultural tradition in the case of China. However, the potential links between compositional capability and the Chinese cultural tradition remain poorly understood and largely unspecified. This paper responds to the call for more research on identifying relevant cultural factors by explicating the inherent connections between compositional capability and the Chinese cultural value of balanced moderation

Daily life affective dynamics as transdiagnostic predictors of mental health symptoms:An ecological momentary assessment study

BackgroundAffective dynamics have been identified as a correlate of a broad span of mental health issues, making them key candidate transdiagnostic factors. However, there remains a lack of knowledge about which aspects of affective dynamics – especially as they manifest in the course of daily life – relate to a general risk for mental health issues versus specific symptoms. MethodsWe leverage an ecological momentary assessment (EMA) study design with four measures per day over a two-week period to explore how negative affect levels, inertia, lability, and reactivity to provocation and stress in the course of daily life relate to mental health symptoms in young adults (n= 256) in the domains of anxiety, depression, psychosis-like symptoms, behaviour problems, suicidality, and substance use. ResultsDynamic structural equation modelling (DSEM) suggested that negative affect levels in daily life were associated with depression, anxiety, indirect and proactive aggression, psychosis, anxiety, and self-injury; negative affective lability was associated with depression, physical aggression, reactive aggression, suicidal ideation, and ADHD symptoms; negative affective inertia was associated with depression, anxiety, physical aggression, and cannabis use; and emotional reactivity to provocation was related to physical aggression. LimitationsThe cross-sectional design, the limited span of mental health issues included, and the convenience nature and small size of the sample are limitations.ConclusionsFindings suggest that a subset of mental health symptoms have shared negative affective dynamics patterns. Longitudinal research is needed to rigorously examine the directionality of the effects underlying the association between affective dynamics and mental health issues

Kids Run the World SHP

The Boys & Girls Club services children ages 6 to 18 with programs and activities that emphasize development strategies (BGCA, n.d.). It provides role models, a safe environment, and constructive activities that focus on overall health. The club depends heavily on community engagement in the form of donors, partnerships, and volunteers (BGCA, n.d.). Our local club provided programs focusing on financial responsibility, leadership, and mental health, but lacked development of physical health practices. Kids Run the World comprised various activities to promote physical health in adolescents. This program is led by college level volunteers that worked with elementary and middle school-aged children to show them fun and safe ways to exercise. Before implementation of the program, research was completed to understand the dynamics of the local Boys & Girls Club. This research included how to be an effective role model and successes of similar programs. This is when “Kid Run the World� was established and put into action. After volunteering at the Boys & Girls Club throughout the duration of our project, we have observed increased physical activity levels and overall morale when group members and athletes are there leading activities. This was successful because of consistent attendance and positive interactions with the children. We learned we needed to have a youthful perspective and engage the kids with exercises they found familiar. Despite COVID-19, our group continues to make efforts to engage youth through our organization on campus, Kids Run the World. The Boys & Girls Club has many underserved children, and this has provided our group with a unique platform that has benefited both us and the children through increased physical activity levels and community involvement

Magnetic resonance spectroscopy reveals N-acetylaspartate reduction in hippocampus and cingulate cortex after fear conditioning

a b s t r a c t The fear conditioning in rodents provides a valuable translational tool to investigate the neural basis of learning and memory and potentially the neurobiology of post-traumatic stress disorder (PTSD). Neurobiological changes induced by fear conditioning have largely been examined ex vivo while progressive 'real-time' changes in vivo remain under-explored. Single voxel proton magnetic resonance spectroscopy ( 1 H MRS) of the hippocampus, cingulate cortex and thalamus of adult male C57BL/6N mice (N¼ 12) was performed at 1 day before, 1 day and 1 week after, fear conditioning training using a 7T scanner. N-acetylaspartate (NAA), a marker for neuronal integrity and viability, significantly decreased in the hippocampus at 1 day and 1 week post-conditioning. Significant NAA reduction was also observed in the cingulate cortex at 1 day post-conditioning. These findings of hippocampal NAA decrease indicate reduced neuronal dysfunction and/or neuronal integrity, contributing to the traumarelated PTSD-like symptoms. The neurochemical changes characterized by 1 H MRS can shed light on the biochemical mechanisms of learning and memory. Moreover, such information can potentially facilitate prompt intervention for patients with psychiatric disorders

Institutions, industries and entrepreneurial versus advantage-based strategies: how complex, nested environments affect strategic choice

With primary and secondary data on 658 firms from 17 countries across three continents, we examine the combined influence of country-level institutions on industry attributes and in turn their effects on the choice of a defensive advantage-based strategy and an entrepreneurial strategy. We find that strong and efficient institutions constrain both dynamism and munificence in industries. In turn, industry dynamism has a negative effect on both entrepreneurial and advantage-based strategies. However, firms having strong relational capital with important stakeholders can better navigate these uncertain environments to employ both strategies with increasing strength. Alternatively, in more munificent environments, firms are less likely to employ advantage-based strategies and more likely to employ entrepreneurial strategies. This study demonstrates the environmental conditions under which firms are more-or-less likely to employ entrepreneurial strategies and defensive advantage-based strategies