3,138 research outputs found
Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains
Background: Homologous recombination mediated by the lambda-Red genes is a common method for making chromosomal modifications in Escherichia coli. Several protocols have been developed that differ in the mechanisms by which DNA, carrying regions homologous to the chromosome, are delivered into the cell. A common technique is to electroporate linear DNA fragments into cells. Alternatively, DNA fragments are generated in vivo by digestion of a donor plasmid with a nuclease that does not cleave the host genome. In both cases the lambda-Red gene products recombine homologous regions carried on the linear DNA fragments with the chromosome. We have successfully used both techniques to generate chromosomal mutations in E. coli K-12 strains. However, we have had limited success with these lambda-Red based recombination techniques in pathogenic E. coli strains, which has led us to develop an enhanced protocol for recombineering in such strains. \ud
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Results: Our goal was to develop a high-throughput recombineering system, primarily for the coupling of genes to epitope tags, which could also be used for deletion of genes in both pathogenic and K-12 E. coli strains. To that end we have designed a series of donor plasmids for use with the lambda-Red recombination system, which when cleaved in vivo by the I-SceI meganuclease generate a discrete linear DNA fragment, allowing for C-terminal tagging of chromosomal genes with a 6xHis, 3xFLAG, 4xProteinA or GFP tag or for the deletion of chromosomal regions. We have enhanced existing protocols and technologies by inclusion of a cassette conferring kanamycin resistance and, crucially, by including the sacB gene on the donor plasmid, so that all but true recombinants are counter-selected on kanamycin and sucrose containing media, thus eliminating the need for extensive screening. This method has the added advantage of limiting the exposure of cells to the potential damaging effects of the lambda-Red system, which can lead to unwanted secondary alterations to the chromosome. \ud
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Conclusion: We have developed a counter-selective recombineering technique for epitope tagging or for deleting genes in E. coli. We have demonstrated the versatility of the technique by modifying the chromosome of the enterohaemorrhagic O157:H7 (EHEC), uropathogenic CFT073 (UPEC), enteroaggregative O42 (EAEC) and enterotoxigenic H10407 (ETEC) E. coli strains as well as in K-12 laboratory strains
Duct growth factor.
Submitted by the senior author in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Graduate School of the University of Missouri, 1939--P. [5].Includes bibliographical references (pages 69-72)
Events leading up to the June 2015 outburst of V404 Cyg
On 2015 June 15 the burst alert telescope (BAT) on board {\em Swift} detected
an X-ray outburst from the black hole transient V404 Cyg. We monitored V404 Cyg
for the last 10 years with the 2-m Faulkes Telescope North in three optical
bands (V, R, and i). We found that, one week prior to this outburst, the
optical flux was 0.1--0.3 mag brighter than the quiescent orbital modulation,
implying an optical precursor to the X-ray outburst. There is also a hint of a
gradual optical decay (years) followed by a rise lasting two months prior to
the outburst. We fortuitously obtained an optical spectrum of V404 Cyg 13 hours
before the BAT trigger. This too was brighter () than
quiescence, and showed spectral lines typical of an accretion disk, with
characteristic absorption features of the donor being much weaker. No He II
emission was detected, which would have been expected had the X-ray flux been
substantially brightening. This, combined with the presence of intense
H emission, about 7 times the quiescent level, suggests that the disk
entered the hot, outburst state before the X-ray outburst began. We propose
that the outburst is produced by a viscous-thermal instability triggered close
to the inner edge of a truncated disk. An X-ray delay of a week is consistent
with the time needed to refill the inner region and hence move the inner edge
of the disk inwards, allowing matter to reach the central BH, finally turning
on the X-ray emission.Comment: Accepted by ApJ Letter, 7 pages, 5 figure
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