9,051 research outputs found

    Prochloron research

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    The purpose was to prepare Prochloron photosynthetic membranes for the isolation of the two major chlorophyll-proteins, the P700-chlorophyll a-protein and the light-harvesting chlorophyll a/b-protein, using SDS-polyacrylamide gel electrophoresis. The prepared proteins (purified) were examined for their cross-reactivity to polyclonal antibodies prepared from higher plant proteins. In addition, material was prepared for electron microscopy, and isolation of the DNA for determination of its general complexity (COT analysis) and similarity to barley chloroplast DNA and Anabaena DNA by using restriction-endonuclease analysis. Kleinschmidt spreads of the DNA were in the electron microscope to identify and measure the extent and size of the circlar DNA

    Adherence to cardiac rehabilitation guidelines: a survey of rehabilitation programmes in the United Kingdom

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    Two key recommendations of recent guidelines are that cardiac rehabilitation requires the skills of a range of professionals and that the patient should receive a menu based programme after an individual assess­ ment of needs. A previous survey of 25 cardiac rehabilitation programmes found little congruence with these guidelines and noted that physicians were particularly unlikely to be involved. We extended this inquiry to include all of the discoverable rehabilitation programmes in the United Kingdom

    Applications of DFT to the theory of twentieth-century harmony

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    Music theorists have only recently, following groundbreaking work by Quinn, recognized the potential for the DFT on pcsets, initially proposed by Lewin, to serve as the foundation of a theory of harmony for the twentieth century. This paper investigates pcset “arithmetic” – subset structure, transpositional combination, and interval content – through the lens of the DFT. It discusses relationships between interval classes and DFT magnitudes, considers special properties of dyads, pcset products, and generated collections, and suggest methods of using the DFT in analysis, including interpreting DFT magnitudes, using phase spaces to understand subset structure, and interpreting the DFT of Lewin’s interval function. Webern’s op. 5/4 and Bartok’s String Quartet 4, iv, are discussed.Accepted manuscrip

    Smart Materials as Intelligent Insulation

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    In order to provide a robust infrastructure for the transmission and distribution of electrical power, understanding and monitoring equipment ageing and failure is of paramount importance. Commonly, failure is associated with degradation of the dielectric material; therefore the introduction of a smart moiety into the material is a potentially attractive means of continual condition monitoring. It is important that any introduction of smart groups into the dielectric does not have any detrimental effect on the desirable electrical and mechanical properties of the bulk material. Initial work focussed on the introduction of fluorophores into a model dielectric system. Fluorescence is known to be a visible effect even at very low concentrations of active fluorophores and therefore was thought well suited to such an application. It was necessary both to optimise the active fluorophore itself and to determine the most appropriate manner in which to introduce the fluorophores into the insulating system. This presentation will describe the effect of introducing fluorophores into polymeric systems on the dielectric properties of the material and the findings thus far [1]. Alternative smart material systems will also be discussed along with the benefits and limitations of smart materials as electric field sensors

    Decontextualizing contextual inversion

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    Contextual inversion, introduced as an analytical tool by David Lewin, is a concept of wide reach and value in music theory and analysis, at the root of neo-Riemannian theory as well as serial theory, and useful for a range of analytical applications. A shortcoming of contextual inversion as it is currently understood, however, is, as implied by the name, that the transformation has to be defined anew for each application. This is potentially a virtue, requiring the analyst to invest the transformational system with meaning in order to construct it in the first place. However, there are certainly instances where new transformational systems are continually redefined for essentially the same purposes. This paper explores some of the most common theoretical bases for contextual inversion groups and considers possible definitions of inversion operators that can apply across set class types, effectively decontextualizing contextual inversions.Accepted manuscrip

    Ground state of two electrons on concentric spheres

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    We extend our analysis of two electrons on a sphere [Phys. Rev. A {\bf 79}, 062517 (2009); Phys. Rev. Lett. {\bf 103}, 123008 (2009)] to electrons on concentric spheres with different radii. The strengths and weaknesses of several electronic structure models are analyzed, ranging from the mean-field approximation (restricted and unrestricted Hartree-Fock solutions) to configuration interaction expansion, leading to near-exact wave functions and energies. The M{\o}ller-Plesset energy corrections (up to third-order) and the asymptotic expansion for the large-spheres regime are also considered. We also study the position intracules derived from approximate and exact wave functions. We find evidence for the existence of a long-range Coulomb hole in the large-spheres regime, and infer that unrestricted Hartree-Fock theory over-localizes the electrons.Comment: 10 pages, 10 figure

    Which way up? Recognition of homologous DNA segments in parallel and antiparallel alignment

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    Homologous gene shuffling between DNA promotes genetic diversity and is an important pathway for DNA repair. For this to occur, homologous genes need to find and recognize each other. However, despite its central role in homologous recombination, the mechanism of homology recognition is still an unsolved puzzle. While specific proteins are known to play a role at later stages of recombination, an initial coarse grained recognition step has been proposed. This relies on the sequence dependence of the DNA structural parameters, such as twist and rise, mediated by intermolecular interactions, in particular electrostatic ones. In this proposed mechanism, sequences having the same base pair text, or are homologous, have lower interaction energy than those sequences with uncorrelated base pair texts; the difference termed the recognition energy. Here, we probe how the recognition energy changes when one DNA fragment slides past another, and consider, for the first time, homologous sequences in antiparallel alignment. This dependence on sliding was termed the recognition well. We find that there is recognition well for anti-parallel, homologous DNA tracts, but only a very shallow one, so that their interaction will differ little from the interaction between two nonhomologous tracts. This fact may be utilized in single molecule experiments specially targeted to test the theory. As well as this, we test previous theoretical approximations in calculating the recognition well for parallel molecules against MC simulations, and consider more rigorously the optimization of the orientations of the fragments about their long axes. The more rigorous treatment affects the recognition energy a little, when the molecules are considered rigid. However when torsional flexibility of the DNA molecules is introduced, we find excellent agreement between analytical approximation and simulation.Comment: Paper with supplemental material attached. 41 pages in all, 4 figures in main text, 3 figures in supplmental. To be submitted to Journa

    XTE J2123-058: A New Neutron Star X-Ray Transient

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    We report on optical and RXTE observations of a new high-latitude bursting X-ray transient, XTE J2123-058. We identified the optical counterpart, measured the 5.9573 hr orbital period and constrained the binary inclination and the source distance. The distance lower limit indicates that the source is at least 2.6 kpc from the Galactic plane, which is unusual for an LMXB. RXTE observations were made between June and August 1998 during the first detected outburst from this source. We describe correlations between X-ray timing and spectral properties and discuss the possibility that the propeller mechanism turned on during the decay of the outburst. During one of the RXTE observations, we detect a pair of high frequency QPOs at 847.1 +/- 5.5 Hz and 1102 +/- 13 Hz simultaneously. According to the beat frequency model, the QPO separation implies a neutron star spin period near 3.9 ms. As the X-ray flux decreases at the end of the outburst, the amplitude of the optical modulation increases significantly. This behavior can be explained if the size of the accretion disk decreases during the decay of the outburst.Comment: 24 pages, 9 figures, accepted by Ap

    XMM-Newton Spectroscopy of the Accretion-Driven Millisecond X-ray Pulsar XTE J1751-305 in Outburst

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    We present an analysis of the first high-resolution spectra measured from an accretion-driven millisecond X-ray pulsar in outburst. We observed XTE J1751-305 with XMM-Newton on 2002 April 7 for approximately 35 ksec. Using a simple absorbed blackbody plus power-law model, we measure an unabsorbed flux of (6.6 +/- 0.1) * 10^(-10) erg/cm^2/s (0.5--10.0 keV). A hard power-law component (Gamma = 1.44 +/- 0.01) contributes 83% of the unabsorbed flux in the 0.5-10.0 keV band, but a blackbody component (kT = 1.05 +/- 0.01 keV) is required. We find no clear evidence for narrow or broad emission or absorption lines in the time-averaged spectra, and the sensitivity of this observation has allowed us to set constraining upper-limits on the strength of important features. The lack of line features is at odds with spectra measured from some other X-ray binaries which share some similarities with XTE J1751-305. We discuss the implications of these findings on the accretion flow geometry in XTE J1751-305.Comment: 5 pages, 3 figures (2 color). ApJ Letters, accepted. Uses emulateapj.st
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