15 research outputs found

    Pipes to Earth's subsurface: the role of atmospheric conditions in controlling air transport through boreholes and shafts

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    Understanding air exchange dynamics between underground cavities (e.g., caves, mines, boreholes, etc.) and the atmosphere is significant for the exploration of gas transport across the Earth–atmosphere interface. Here, we investigated the role of atmospheric conditions in controlling air transport inside boreholes using in situ field measurements. Three geometries were explored: (1) a narrow and deep shaft (0.1&thinsp;m wide and 27&thinsp;m deep), ending in a large underground cavity; (2) the same shaft after the pipe was lowered and separated from the cavity; and (3) a deep large-diameter borehole (59&thinsp;m deep and 3.4&thinsp;m wide). Absolute humidity was found to be a reliable proxy for distinguishing between atmospheric and cavity air masses (mainly during the winter and spring seasons) and thus to explore air transport through the three geometries. Airflow directions in the first two narrow-diameter geometries were found to be driven by changes in barometric pressure, whereas airflow in the large-diameter geometry was correlated primarily with the diurnal cycles of ambient atmospheric temperature. CO2 concentrations of  ∼ 2000&thinsp;ppm were found in all three geometries, indicating that airflow from the Earth's subsurface into the atmosphere may also be significant in the investigation of greenhouse gas emissions.</p

    Molecular and Dual-Isotopic Profiling of the Microbial Controls on Nitrogen Leaching in Agricultural Soils under Managed Aquifer Recharge

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    Nitrate (NO3–) leaching is a serious health and ecological concern in global agroecosystems, particularly those under the application of agricultural-managed aquifer recharge (Ag-MAR); however, there is an absence of information on microbial controls affecting NO3– leaching outcomes. We combine natural dual isotopes of NO3– (15N/14N and 18O/16O) with metagenomics, quantitative polymerase chain reaction (PCR), and a threshold indicator taxa analysis (TITAN) to investigate the activities, taxon profiles, and environmental controls of soil microbiome associated with NO3– leaching at different depths from Californian vineyards under Ag-MAR application. The isotopic signatures demonstrated a significant priming effect (P < 0.01) of Ag-MAR on denitrification activities in the topsoil (0–10 cm), with a 12–25-fold increase of 15N–NO3– and 18O–NO3– after the first 24 h of flooding, followed by a sharp decrease in the enrichment of both isotopes with ∼80% decline in denitrification activities thereafter. In contrast, deeper soils (60–100 cm) showed minimal or no denitrification activities over the course of Ag-MAR application, thus resulting in 10–20-fold of residual NO3– being leached. Metagenomic profiling and laboratory microcosm demonstrated that both nitrifying and denitrifying groups, responsible for controlling NO3– leaching, decreased in abundance and potential activity rates with soil depth. TITAN suggested that Nitrosocosmicus and Bradyrhizobium, as the major nitrifier and denitrifier, had the highest and lowest tipping points with regard to the NO3– changes (P < 0.05), respectively. Overall, our study provides new insight into specific depth limitations of microbial controls on soil NO3– leaching in agroecosystems
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