Development of a candidate reference material for adventitious virus detection in vaccine and biologicals manufacturing by deep sequencing.

Unbiased deep sequencing offers the potential for improved adventitious virus screening in vaccines and biotherapeutics. Successful implementation of such assays will require appropriate control materials to confirm assay performance and sensitivity. A common reference material containing 25 target viruses was produced and 16 laboratories were invited to process it using their preferred adventitious virus detection assay. Fifteen laboratories returned results, obtained using a wide range of wet-lab and informatics methods. Six of 25 target viruses were detected by all laboratories, with the remaining viruses detected by 4-14 laboratories. Six non-target viruses were detected by three or more laboratories. The study demonstrated that a wide range of methods are currently used for adventitious virus detection screening in biological products by deep sequencing and that they can yield significantly different results. This underscores the need for common reference materials to ensure satisfactory assay performance and enable comparisons between laboratories

Mise au point de virus variole aviaire recombinants, construction et utilisation chez la volaille d'un virus recombinannt la glycoprotéine de fusion du virus de la maladie de Newcastle

Doctorat en Sciencesinfo:eu-repo/semantics/nonPublishe

Classification des systèmes dynamiques par circuits de rétroaction

info:eu-repo/semantics/publishe

Periparturient infection with bovine viral diarrhea virus type 1 causes hemorrhagic proctocolitis in a cow

After 3 cows of a dairy herd had died from severe hemorrhagic diarrhea, a 4th sick cow was transported to the clinic. Blood analyses revealed the complete absence of white blood cells, the presence of a type 1b strain of bovine viral diarrhea virus (BVDV), and seroconversion to BVDV

Importance of the F protein in the immunity of the Newcastle disease virus

Des anticorps monoclonaux dirigés contre les deux glycoprotéines de surface HN et F du virus de la maladie de Newcastle ont été préparés. Nous les avons caractérisés par des tests d’inhibition de l’hémagglutination, de séro neutralisation et de radio-immunoprécipitation. Le rôle de ces deux types d’anticorps dans l’immunité au virus de la maladie de Newcastle a été évalué in vitro dans des tests de séroneutralisation et in vivo dans des épreuves d’immunisation passive de volailles EOPS. Des anticorps monoclonaux anti-F confèrent une protection passive plus élevée que les anticorps anti-HN ; et de plus, ils neutralisent toutes les souches virales représentatives des divers sous-groupes de virus Newcastle. Un virus vaccine recombinant exprimant la protéine F du virus de la maladie de Newcastle a été obtenu par manipulation génétique. Les résultats préliminaires de vaccination à l’aide de ce recombinant démontrent l’intérêt potentiel de l’utilisation de vaccins Newcastle à base de protéine F.Monoclonal antibodies directed against HN and F, the two surface glyco proteins of Newcastle disease virus were prepared and characterized using hemagglutination inhibition, séroneutralisation and radio-immunoprecipitation tests. The relative importance of HN and F antibodies in the immunity against Newcastle disease virus was estimated « in vitro » by séroneutralisation and « in vivo » by passive immunisation of SPF chickens. Monoclonal antibodies directed against the F protein are more potent in passive immunisation tests than HN antibodies ; moreover, they neutralize all representative strains of the different subgroups of Newcastle disease virus. A recombinant vaccinia virus expressing the F protein of Newcastle disease virus was obtained by genetic engineering. Preliminary vaccination results using this recombinant demonstrate the potential interest of the use of Newcastle disease vaccines based on the F protein

Circulation in Belgium of a bovine viral diarrhoea virus type 2 closely related to North American hypervirulent viruses

A

Newcastle disease virus f glycoprotein expressed from a recombinant vaccinia virus vector protects chickens against live-virus challenge

Chickens were immunised using a vaccinia recombinant virus (vaccinia-Italien-F), expressing the F protein of Newcastle disease virus (NDV). Immunisation was successful using either TK cells infected with the vaccinia-Italien-F virus, the recombinant virus grown in tk cells and inoculated intra-cerebrally in one-day-old chickens or the recombinant virus given by wing-web to adult chickens after adaptation by alternate passage in chick embryo fibroblasts and chickens. The use of recombinant viruses expressing the F protein of NDV as vaccines would allow joint application of vaccination and eradication programmes for NDV. Therefore, recombinant viruses obtained in chickens virus vectors are needed. © 1988, Taylor & Francis Group, LLC. All rights reserved.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Development of a 1-step enzyme-linked immunosorbent assay for the rapid diagnosis of bovine respiratory syncytial virus in postmortem specimens.

Bovine respiratory syncytial virus (BRSV) is associated with severe respiratory disease in cattle. BRSV infection frequently leads to the death of young infected animals. The presence of BRSV in postmortem specimens is routinely detected using indirect immunofluorescence (IIF). However, this technique requires special equipment and considerable expertise. The present paper describes the development of a 1-step ELISA for rapid (1.5 hours) detection of BRSV antigen in organ homogenates. The performance of the new 1-step ELISA was evaluated using bovine postmortem specimens (n = 108) in comparison with 3 other BRSV diagnostic techniques: indirect immunofluorescence, the Clearview respiratory syncytial virus (RSV) test, and real-time reverse transcriptase polymerase chain reaction (RT-PCR). The relative sensitivity, specificity, and the kappa coefficient of 1-step ELISA, the Clearview RSV electroimmunoassay (EIA), and IIF were calculated, using real-time RT-PCR as the reference test. The new 1-step ELISA was the most sensitive and specific of the 3 tests. Thus, the new 1-step ELISA is a reliable test for detecting BRSV antigen in organ homogenates