Mechanical design of the superconducting solenoid package for the SARAF-linac project

International audienc

Toward a Scalable Purification Protocol of GaLV-TR-Pseudotyped Lentiviral Vectors

International audienceLentiviral vectors (LV) that are used in research and development as well as in clinical trials are in majority vesicular stomatitis virus G glycoprotein (VSVg) pseudotyped. The predominance of this pseudotype choice for clinical gene therapy studies is largely due to a lack of purification schemes for pseudotypes other than VSVg. In this study, we report for the first time the development of a new downstream process protocol allowing high-yield production of stable and infectious gibbon ape leukemia virus (GaLV)-TR-LV particles. We identified critical conditions in tangential flow filtration (TFF) and chromatographic steps for preserving the infectivity/functionality of LV during purification. This was carried out by identifying for each step, the critical parameters affecting LV infectivity, including pH, salinity, presence of stabilizers, temperature, and by defining the optimal order of these steps. A three-step process was developed for GaLV-TR-LV purification consisting of one TFF and two chromatographic steps (ion-exchange chromatography and size exclusion chromatography) permitting recoveries of >27% of infectious particles. With this process, purified GaLV-pseudotyped LV enabled the transduction of 70% human CD34(+) cells in the presence of the Vectofusin-1 peptide, whereas in the same conditions nonpurified vector transduced only 9% of the cells (multiplicity of infection 20). Our protocol will allow for the first time the purification of GaLV-TR-LV that are biologically active, stable, and with sufficient recovery in the perspective of preclinical studies and clinical applications. Obviously, further optimizations are required to improve final vector yields

Existe-t-il vraiment une psychopathologie du rabâchage chez le sujet agé ?

International audienceRepetitive talk has not been widely studied from the semeiotic and neuro-psychological angle, although it is a marker in the loss of control of the environment with old age. In this study a group of elderly, anxious subjects, a group of elderly subjects with cognitive disturbances and a group of elderly controls were compared. A quantitative analysis of repetitive speech, using the SRI (Stereotyping Rating Inventory) was performed, followed by a qualitative analysis, using indicators of memory, executive functions (updating; flexibility; inhibition) personality, anxiety and depression. Among subjects with cognitive and anxious disturbances, repetitive talk comprised stories (more than 2 or 3 sentences) or sentences, but there was no repetition of words or songs. The encounter with others is affected by this repetitive talk, and results from the fact that either the discourse is centered on completed events (because of difficulties in updating in the cognitive disturbance group), or it incorporates superfluous, diffuse events, also resulting from an impairment in updating abilities, but also from inhibition and mental flexibility problems in the group of anxious subjects.Le rabâchage, pris au sens de discours répétitif, a été peu étudié au plan sémiolo-gique et neuropsychologique, même s'il représente une étape charnière de la perte du contrôle de l'environnement, avec la vieillesse. Dans ce travail sont comparés un groupe de sujets âgés anxieux et un groupe de sujets âgés ayant des troubles cognitifs et un groupe de sujets âgés contrôles. Il a été fait une analyse quantitative du discours répétitif, au moyen du Stereotyping Rating Inventory (SRI) et une analyse qualitative du discours répétitif, au moyen d'indicateurs de mémoire, de fonctions exécutives (mise à jour, flexibilité, inhibition), de personnalité et d'anxiété-dépression. Pour ceux ayant des troubles cognitifs et les anxieux, le rabâchage est plutôt composé d'histoires (plus de 2 ou 3 phrases) ou de phrases mais pas de répétition concer-nant des mots ou des chansons. L'altération de la rencontre avec l'autre, du fait de ces discours répétitifs, se fait soit parce qu'il est centré sur des évènements terminés (par atteinte des capa-cités de mise à jour, en ce qui concerne notre groupe ayant des troubles cognitifs), soit parce qu'il intègre dans le discours des évènements superflus, diffluents, issus d'une atteinte des capacités de mise à jour, mais aussi d'inhibition et de flexibilité mentale, dans notre groupe d'anxieux

Influence of ball-milling and annealing conditions on nanocluster characteristics in oxide dispersion strengthened steels

International audienc

Influence of ball-milling and annealing conditions on nanocluster characteristics in oxide dispersion strengthened steels

International audienc

ESS Elliptical Cavities and Cryomodules

The accelerator of the European Spallation Source (ESS) is a 5 MW proton linac to be built in Lund Sweden. Its superconducting section is composed of 3 cavity families: double spoke resonators, medium beta and high beta elliptical multi-cell cavities. This paper presents the electromagnetic and mechanical design of the elliptical cavities. Both elliptical families are housed in 4-cavity cryomodules which share a common design and set of components which will be described here