108 research outputs found

    Geometric constrains for detecting short actin filaments by cryogenic electron tomography

    Get PDF
    Polymerization of actin into filaments can push membranes forming extensions like filopodia or lamellipodia, which are important during processes such as cell motility and phagocytosis. Similarly, small organelles or pathogens can be moved by actin polymerization. Such actin filaments can be arranged in different patterns and are usually hundreds of nanometers in length as revealed by various electron microscopy approaches. Much shorter actin filaments are involved in the motility of apicomplexan parasites. However, these short filaments have to date not been visualized in intact cells. Here, we investigated Plasmodium sporozoites, the motile forms of the malaria parasite that are transmitted by the mosquito, using cryogenic electron tomography. We detected filopodia-like extensions of the plasma membrane and observed filamentous structures in the supra-alveolar space underneath the plasma membrane. However, these filaments could not be unambiguously assigned as actin filaments. In silico simulations of EM data collection and tomographic reconstruction identify the limits in revealing the filaments due to their length, concentration and orientation

    Π’Π΅Ρ…Π½ΠΎΠ³Π΅Π½Π½Ρ‹Π΅ мСстороТдСния, ΡΡ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π²ΡˆΠΈΠ΅ΡΡ Π½Π° ΠΎΠ±ΡŠΠ΅ΠΊΡ‚Π°Ρ… Π³ΠΎΡ€Π½ΠΎΠΏΡ€ΠΎΠΌΡ‹ΡˆΠ»Π΅Π½Π½ΠΎΠ³ΠΎ производства Π² Π₯акасии

    Get PDF
    ΠžΡ…Π°Ρ€Π°ΠΊΡ‚Π΅Ρ€ΠΈΠ·ΠΎΠ²Π°Π½Ρ‹ Π½Π΅ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ Ρ‚Π΅Ρ…Π½ΠΎΠ³Π΅Π½Π½Ρ‹Π΅ ΠΎΠ±ΡŠΠ΅ΠΊΡ‚Ρ‹ Π³ΠΎΡ€Π½ΠΎΠΏΡ€ΠΎΠΌΡ‹ΡˆΠ»Π΅Π½Π½Ρ‹Ρ… прСдприятий Π₯акасии, ΡΠ²Π»ΡΡŽΡ‰ΠΈΠ΅ΡΡ источником воздСйствия Π½Π° ΠΎΠΊΡ€ΡƒΠΆΠ°ΡŽΡ‰ΡƒΡŽ срСду ΠΈ пСрспСктивныС для изучСния Π½Π° ΠΏΡ€Π΅Π΄ΠΌΠ΅Ρ‚ получСния Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ

    ΠŸΠ»Π°Π½ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ производствСнной ΠΏΡ€ΠΎΠ³Ρ€Π°ΠΌΠΌΡ‹ Π½Π° прСдприятии

    Get PDF
    Дипломная Ρ€Π°Π±ΠΎΡ‚Π° содСрТит 91 страницу,8 рисунков, 28 Ρ‚Π°Π±Π»ΠΈΡ†, 20 ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΠΎΠ²Π°Π½Π½Ρ‹Ρ… источников, 3 прилоТСния. ΠšΠ»ΡŽΡ‡Π΅Π²Ρ‹Π΅ слова: производствСнная ΠΏΡ€ΠΎΠ³Ρ€Π°ΠΌΠΌΠ°, выпуск ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ, ассортимСнт, Ρ€ΠΈΡ‚ΠΌΠΈΡ‡Π½ΠΎΡΡ‚ΡŒ производства, ΠΏΠΎΡ€Ρ‚Ρ„Π΅Π»ΡŒ Π·Π°ΠΊΠ°Π·ΠΎΠ², производствСнная ΠΌΠΎΡ‰Π½ΠΎΡΡ‚ΡŒ. ΠžΠ±ΡŠΠ΅ΠΊΡ‚ΠΎΠΌ исслСдования являСтся ОАО Β«ΠšΠ΅ΠΌΠ΅Ρ€ΠΎΠ²ΡΠΊΠΈΠΉ ΠΌΠΎΠ»ΠΎΡ‡Π½Ρ‹ΠΉ ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Ρ‚Β». ЦСль Π΄ΠΈΠΏΠ»ΠΎΠΌΠ½ΠΎΠΉ Ρ€Π°Π±ΠΎΡ‚Ρ‹ - ΠΏΠ»Π°Π½ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ производствСнной ΠΏΡ€ΠΎΠ³Ρ€Π°ΠΌΠΌΡ‹ прСдприятия для ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΡ Π΅Π³ΠΎ Ρ€Π΅Π½Ρ‚Π°Π±Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΠΈ. ΠœΠ΅Ρ‚ΠΎΠ΄Ρ‹ исслСдований: аналитичСский, расчСтный, графичСский. Π’ процСссС исслСдования ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈΡΡŒ Π°Π½Π°Π»ΠΈΠ· выполнСния ΠΏΠ»Π°Π½Π° ΠΏΠΎ выпуску ΠΈ Ρ€Π΅Π°Π»ΠΈΠ·Π°Ρ†ΠΈΠΈ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ, ритмичности производства, качСства ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ, срСды прСдприятия, портфСля Π·Π°ΠΊΠ°Π·ΠΎΠ², использования производствСнных мощностСй. Π’ Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π΅ исслСдования ΠΎΠΏΡ€Π΅Π΄Π΅Π»Π΅Π½ ΠΏΠ»Π°Π½ выпуска ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ Π² Π½Π°Ρ‚ΡƒΡ€Π°Π»ΡŒΠ½ΠΎΠΌ ΠΈ стоимостном Π²The thesis contains a 91-page,8 figures, 28 tables, 20 of references, 3 appendices. Keywords: production program, production, range, rhythm of production, the portfolio of orders, production capacity. The object of study is OJSC "Kemerovo dairy industrial complex". The aim of the thesis - planning the production program of the enterprise to improve its profitability. Research methods: analytical, design, graphics. In the process of investigation the analysis of the plan for the production and sale of products, the rhythm of production, product quality, environment, backlog of orders, capacity utilization. The study set out a plan of issue of products in physical and value terms, reserves of improvement of capacity utilization, assessed the effectiveness of the production program. Level o

    Π Π°Π·Ρ€Π°Π±ΠΎΡ‚ΠΊΠ° Ρ‚Π΅Ρ…Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ изготовлСния Π΄Π΅Ρ‚Π°Π»ΠΈ «КолСсо Π·ΡƒΠ±Ρ‡Π°Ρ‚ΠΎΠ΅Β»

    Get PDF
    Π—ΡƒΠ±Ρ‡Π°Ρ‚Ρ‹Π΅ колСса ΡˆΠΈΡ€ΠΎΠΊΠΎ ΠΏΡ€ΠΈΠΌΠ΅Π½ΡΡŽΡ‚ΡΡ Π² ΠΌΠ°ΡˆΠΈΠ½ΠΎΡΡ‚Ρ€ΠΎΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠΉ отрасли, благодаря Π½ΠΈΠΌ осущСствляСтся ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ усилий ΠΏΠ΅Ρ€Π΅Π΄Π°Π²Π°Π΅ΠΌΠΎΠ΅ ΠΎΡ‚ ΠΈΡΠΏΠΎΠ»Π½ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Ρ… ΠΌΠ΅Ρ…Π°Π½ΠΈΠ·ΠΌΠΎΠ². Π Π°Π·Ρ€Π°Π±ΠΎΡ‚ΠΊΠ° тСхнологичСского процСсса изготовлСния Π΄Π΅Ρ‚Π°Π»ΠΈ - ваТная ΡΠΎΡΡ‚Π°Π²Π»ΡΡŽΡ‰Π°Ρ Ρ‡Π°ΡΡ‚ΡŒ производствСнного процСсса. ΠžΠ±Π΅ΡΠΏΠ΅Ρ‡Π΅Π½ΠΈΠ΅ ΠΏΡ€ΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΠΈ ΠΏΡ€ΠΈ Ρ€Π°Π·Ρ€Π°Π±ΠΎΡ‚ΠΊΠ΅ Ρ‚Π΅Ρ…Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ Π½Π΅Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ ΠΎΡΡƒΡ‰Π΅ΡΡ‚Π²ΠΈΡ‚ΡŒ Π±Π΅Π· Ρ€Π΅ΡˆΠ΅Π½ΠΈΡ ряда Π·Π°Π΄Π°Ρ‡, ΠΊ ΠΊΠΎΡ‚ΠΎΡ€Ρ‹ΠΌ относятся: Π²Ρ‹Π±ΠΎΡ€ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠ² ΠΎΠ±Ρ€Π°Π±ΠΎΡ‚ΠΊΠΈ повСрхностСй; Π²Ρ‹Π±ΠΎΡ€ тСхнологичСских Π±Π°Π·; расчСты ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡŒΠ½Ρ‹Ρ… припусков, Ρ€Π΅ΠΆΠΈΠΌΠΎΠ² рСзания; Π²Ρ‹Π±ΠΎΡ€ оборудования ΠΈ тСхнологичСской оснастки, Ρ€Π΅ΠΆΡƒΡ‰Π΅Π³ΠΎ инструмСнта ΠΈ Π½ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΎΠΏΠ΅Ρ€Π°Ρ†ΠΈΠΉ тСхнологичСского процСсса. Π’ Π΄Π°Π½Π½ΠΎΠΉ Ρ€Π°Π±ΠΎΡ‚Π΅ Ρ€Π°ΡΡΠΌΠ°Ρ‚Ρ€ΠΈΠ²Π°ΡŽΡ‚ΡΡ ΠΈ Ρ€Π΅ΡˆΠ°ΡŽΡ‚ΡΡ всС поставлСнныС Π·Π°Π΄Π°Ρ‡ΠΈ.Gear wheels are widely used in the engineering industry, thanks to them, a change in the forces transmitted from the actuators is carried out. The development of a technological process for manufacturing a part is an important part of the production process. Provision of productivity in the development of technology cannot be carried out without solving a number of problems, which include: selection of surface treatment methods; selection of technological bases; calculations of minimum allowances, cutting conditions; selection of equipment and technological equipment, cutting tools and standardization of technological process operations. In this work, all the tasks are considered and solved

    Social orientation of banks

    Get PDF
    The influence of the bank's orientation on clients and the quality of the services provided on the financial efficiency of the commercial institution (sales volume, profitability, cost reduction) was studied. The development of online banking and Internet banking is considered. The relationship between the profitability of the bank and the level of customercentricity is proved. World experience has been compared and generalized, and country specificity has been singled out in different regions of the world. The dependence between the degree of customer satisfaction and their subsequent loyalty to the bank was studied. Prospective directions of expansion of the market of banking services are outlined, and also changes in the psychology of the client that took place due to scientific and technological progress are noted. The basic directions of increase of efficiency of work of the bank personnel are allocated

    ИсслСдованиС ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠ² измСрСния Ρ‚ΠΎΠ»Ρ‰ΠΈΠ½Ρ‹ стСнки лСгкосплавных Π±ΡƒΡ€ΠΈΠ»ΡŒΠ½Ρ‹Ρ… Ρ‚Ρ€ΡƒΠ±

    Get PDF
    Π’ΠΎΠ»Ρ‰ΠΈΠ½Π° стСнки Π±ΡƒΡ€ΠΈΠ»ΡŒΠ½ΠΎΠΉ Ρ‚Ρ€ΡƒΠ±Ρ‹ ΠΈΠ· алюминиСвого сплава измСряСтся Π²ΠΈΡ…Ρ€Π΅Ρ‚ΠΎΠΊΠΎΠ²Ρ‹ΠΌ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ, акустичСским ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ, ΠΌΠ°Π³Π½ΠΈΡ‚Π½Ρ‹ΠΌ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ, Π Π°Π΄ΠΈaΡ†ΠΈΠΎΠ½Π½Ρ‹ΠΌ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ ΠΈ Π²ΠΈΠ·ΡƒΠ°Π»ΡŒΠ½ΠΎ-оптичСским ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ. ΠŸΡ€ΠΈΠ½Ρ†ΠΈΠΏ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‰Π΅Π³ΠΎ инструмСнта Ρ‚Π°ΠΊΠΆΠ΅ описываСтся с ΠΏΠΎΠΌΠΎΡ‰ΡŒΡŽ ΠΏΡ€ΠΈΠ½Ρ†ΠΈΠΏΠ° Π²Ρ‹ΡˆΠ΅ΡƒΠΏΠΎΠΌΡΠ½ΡƒΡ‚ΠΎΠ³ΠΎ ΠΌΠ΅Ρ‚ΠΎΠ΄Π°.Through the eddy current method, acoustic method, electromagnetic method, radiation method, visual optical method, to measure the thickness of the aluminum alloy drill pipe wall. The corresponding instrument principles are also described through the principles of the above methods
    • …
    corecore