1,541 research outputs found
Study of the optimal conditions for NV- center formation in type 1b diamond, using photoluminescence and positron annihilation spectroscopies
We studied the parameters to optimize the production of negatively-charged
nitrogen-vacancy color centers (NV-) in type~1b single crystal diamond using
proton irradiation followed by thermal annealing under vacuum. Several samples
were treated under different irradiation and annealing conditions and
characterized by slow positron beam Doppler-broadening and photoluminescence
(PL) spectroscopies. At high proton fluences another complex vacancy defect
appears limiting the formation of NV-. Concentrations as high as 2.3 x 10^18
cm^-3 of NV- have been estimated from PL measurements. Furthermore, we inferred
the trapping coefficient of positrons by NV-. This study brings insight into
the production of a high concentration of NV- in diamond, which is of utmost
importance in ultra-sensitive magnetometry and quantum hybrid systems
applications
Dislocation interactions mediated by grain boundaries
The dynamics of dislocation assemblies in deforming crystals indicate the
emergence of collective phenomena, intermittent fluctuations and strain
avalanches. In polycrystalline materials, the understanding of plastic
deformation mechanisms depends on grasping the role of grain boundaries on
dislocation motion. Here the interaction of dislocations and elastic, low angle
grain boundaries is studied in the framework of a discrete dislocation
representation. We allow grain boundaries to deform under the effect of
dislocation stress fields and compare the effect of such a perturbation to the
case of rigid grain boudaries. We are able to determine, both analytically and
numerically, corrections to dislocation stress fields acting on neighboring
grains, as mediated by grain boundary deformation. Finally, we discuss
conclusions and consequences for the avalanche statistics, as observed in
polycrystalline samples.Comment: 13 pages, 5 figure
Vibrio parahaemolyticus control in mussels by a Halobacteriovorax isolated from the Adriatic sea, Italy
This study evaluated the application of a Halobacteriovorax isolated from water of the Adriatic Sea (Italy) in controlling V. parahaemolyticus in mussels (Mytilus galloprovincialis). Two 72 h laboratory-scale V. parahaemolyticus decontamination experiments of mussels were performed. The test microcosm of experiment 1 was prepared using predator/prey free mussels experimentally contaminated with Halobacteriovorax/V. parahaemolyticus at a ratio of 103 PFU/105 CFU per ml, while that of experiment 2 using mussels naturally harbouring Halobacteriovorax that were experimentally contaminated with 105 CFU per ml of V. parahaemolyticus. For experiment 1, was also tested a control microcosm only contaminated with 105 CFU per ml of V. parahaemolyticus. Double layer agar plating and pour plate techniques were used to enumerate Halobacteriovorax and V. parahaemolyticus, respectively. 16 S rRNA analysis was used to identify Halobacteriovorax. For both experiments in the test microcosm the concentration of prey remained at the same level as that experimentally added, i.e. 5 log for the entire analysis period. In experiment 1, V. parahaemolyticus counts in mussels were significantly lower in the test microcosm than the control with the maximum difference of 2.2 log at 24 h. Results demonstrate that Halobacteriovorax can modulate V. parahaemolyticus level in the mussels. The public impact of V. parahaemolyticus in bivalves is relevant and current decontamination processes are not always effective. Halobacteriovorax is a suitable candidate in the development of a biological approach to the purification of V. parahaemolyticus in mussels
GDR Feeding of the Highly-Deformed Band in 42Ca
The gamma-ray spectra from the decay of the GDR in the compound nucleus
reaction 18O+28Si at bombarding energy of 105 MeV have been measured in an
experiment using the EUROBALL IV and HECTOR arrays. The obtained experimental
GDR strength function is highly fragmented, with a low energy (10 MeV)
component, indicating a presence of a large deformation and Coriolis effects.
In addition, the preferential feeding of the highly-deformed band in 42Ca by
this GDR low energy component is observed.Comment: 6 pages, 2 figures, Proceedings of the Zakopane2004 Symposium, to be
published in Acta Phys. Pol. B36 (2005
Galleria mellonella as an infection model to investigate virulence of Vibrio parahaemolyticus
This is the final version. Available from Taylor & Francis via the DOI in this recordNon-toxigenic V. parahaemolyticus isolates (tdh-/trh-/T3SS2-) have recently been isolated from patients with gastroenteritis. In this study we report that the larvae of the wax moth (Galleria mellonella) are susceptible to infection by toxigenic or non-toxigenic clinical isolates of V. parahaemolyticus. In comparison larvae inoculated with environmental isolates of V. parahaemolyticus did not succumb to disease. Whole genome sequencing of clinical non-toxigenic isolates revealed the presence of a gene encoding a nudix hydrolase, identified as mutT. A V. parahaemolyticus mutT mutant was unable to kill G. mellonella at 24 h post inoculation, indicating a role of this gene in virulence. Our findings show that G. mellonella is a valuable model for investigating screening of possible virulence genes of V. parahaemolyticus and can provide new insights into mechanisms of virulence of atypical non-toxigenic V. parahaemolyticus. These findings will allow improved genetic tests for the identification of pathogenic V. parahaemolyticus to be developed and will have a significant impact for the scientific community.This work was partly supported by Wellcome Trust Institutional Strategic Support Fund (WT097835MF), Wellcome Trust Multi User Equipment Award (WT097835MF) Medical Research Council Clinical Infrastructure Funding (MR/M008924/1) and Biotechnology and Biological Sciences Research Council (BBSRC) funding (BB/N016513/1)
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