311 research outputs found
Reducing dietary wild derived fishmeal inclusion levels in production diets for large yellowtail kingfish (Seriola lalandi)
Further research to understand the effect of dietary wild derived fishmeal (WD-FM) substitution with commercially relevant alternative ingredients for large yellowtail kingfish (Seriola lalandi; YTK) was investigated. This 36-week study was designed to replace dietary inclusions of WD-FM with alternative protein ingredients including poultry meal, soy protein concentrate and by-product fishmeal (PM, SPC and BP-FM) and measure the effect on the growth performance, feed utilisation, and health of large YTK (2.5 kg initial weight) at ambient water temperatures (average 16.6 °C). Six diets were formulated on a digestible basis to contain 39% digestible protein (âŒ45â46% crude protein), 23% digestible lipid (âŒ24â25% crude lipid), and a digestible energy level of 17 MJ kgâ1 (âŒ19 MJ kgâ1 gross energy level). Fish were fed to apparent satiation once daily at 10:00 h. Substitution of fish meal with alternative ingredients did not significantly impact fish growth, feed utilisation, gastrointestinal health, blood haematology or measured biochemistry indices. Results from the current study will allow reductions to the dietary WD-FM inclusion levels, with tangible sustainability benefits. The inclusion of the alternative protein sources resulted in improvements in the fish in-fish out ratios of up to 35.1%. This study suggests formulation criteria for large YTK should include a minimum of 10% WD-FM. Further to this, at least 30% of the diet should consist of a combination of poultry meal, soy protein concentrate and fishmeal (both wild and by-product). Our data further support the use of BP-FM up to âŒ20% inclusion, while PM and SPC should be limited to âŒ10% inclusion until further data is available on these raw materials in YTK feeds. These recommendations will facilitate formulation flexibility for large YTK feeds, enabling formulators to adapt to changes to extrinsic factors such as raw material availability, and sustainability while minimising cost and performance impacts
Arabidopsis in the wildâthe effect of seasons on seed performance
Climate changes play a central role in the adaptive life histories of organisms all over the world. In higher plants, these changes may impact seed performance, both during seed development and after dispersal. To examine the plasticity of seed performance as a response to environmental fluctuations, eight genotypes known to be affected in seed dormancy and longevity were grown in the field in all seasons of two years. Soil and air temperature, day length, precipitation, and sun hours per day were monitored. We show that seed performance depends on the season. Seeds produced by plants grown in the summer, when the days began to shorten and the temperature started to decrease, were smaller with deeper dormancy and lower seed longevity compared to the other seasons when seeds were matured at higher temperature over longer days. The performance of seeds developed in the different seasons was compared to seeds produced in controlled conditions. This revealed that plants grown in a controlled environment produced larger seeds with lower dormancy than those grown in the field. All together the results show that the effect of the environment largely overrules the genetic effects, and especially, differences in seed dormancy caused by the different seasons were larger than the differences between the genotypes.</p
Role of tumor-derived fibroblasts in the growth of primary cultures of human breast-cancer cells: Effects of epidermal growth factor and the somatostatin analogue octreotide
In the present study we have investigated the role of human breast-cancer-derived fibroblasts in the proliferation of primary cultures of epithelial cells derived from the same tumor. For this purpose, a co-culture system, using Transwell tissue-culture inserts with microporous membranes was employed. Fibroblasts and epithelial cells were enriched according to differences in their density on Percoll density gradients. The co-culture system was first established using MCF-7 breast cancer cells and a human fibroblast line (HF cells). Insulin, 17ÎČ-estradiol, EGF and HF cells all significantly stimulated the growth of MCF-7 breast cancer cells. The stimulatory effects of insulin, E2 and EGF were additive to the stimulatory effect of HF cells. These data suggest that (unique) factor(s), other than the above-mentioned growth-promoting compounds, are responsible for the growth-promoting effects of fibroblasts. In half of the human breast cancers investigated, tumor-derived fibroblasts stimulated tumor-derived epithelial cell proliferation. EGF significantly stimulated epithelial cell proliferation in 4 out of 6 cultures. The stimulatory effects of fibroblasts and EGF were additive or synergistic, and were observed in the additional presence of FCS, again suggesting production of unique factor(s) by the fibroblasts, in one culture the fibroblasts significantly inhibited epithelial tumor-cell proliferation. Conversely, the epithelial cells significantly stimulated proliferation of fibroblasts in 3 out of 3 cultures. The somatostatin analogue octreotide significantly inhibited epithelial cell proliferation by 46% in one tumor-cell culture in the absence, but not in the presence, of fibroblasts. In one culture, octreotide significantly inhibited the proliferation of fibroblasts co-cultured with epithelial cells
Sculpting the Extra Dimensions: Inflation from Codimension-2 Brane Back-reaction
We construct an inflationary model in 6D supergravity that is based on
explicit time-dependent solutions to the full higher-dimensional field
equations, back-reacting to the presence of a 4D inflaton rolling on a
space-filling codimension-2 source brane. Fluxes in the bulk stabilize all
moduli except the `breathing' modulus (that is generically present in
higher-dimensional supergravities). Back-reaction to the inflaton roll causes
the 4D Einstein-frame on-brane geometry to expand, a(t) ~ t^p, as well as
exciting the breathing mode and causing the two off-brane dimensions to expand,
r(t) ~ t^q. The model evades the general no-go theorems precluding 4D de Sitter
solutions, since adjustments to the brane-localized inflaton potential allow
the power p to be dialed to be arbitrarily large, with the 4D geometry becoming
de Sitter in the limit p -> infinity (in which case q = 0). Slow-roll solutions
give accelerated expansion with p large but finite, and q = 1/2. Because the
extra dimensions expand during inflation, the present-day 6D gravity scale can
be much smaller than it was when primordial fluctuations were generated -
potentially allowing TeV gravity now to be consistent with the much higher
gravity scale required at horizon-exit for observable primordial gravity waves.
Because p >> q, the 4 on-brane dimensions expand more quickly than the 2
off-brane ones, providing a framework for understanding why the observed four
dimensions are presently so much larger than the internal two. If uplifted to a
10D framework with 4 dimensions stabilized, the 6D evolution described here
could describe how two of the six extra dimensions evolve to become much larger
than the others, as a consequence of the enormous expansion of the 4 large
dimensions we can see.Comment: 27 pages + appendices, 2 figure
Exceptionally Slow Rise in Differential Reflectivity Spectra of Excitons in GaN: Effect of Excitation-induced Dephasing
Femtosecond pump-probe (PP) differential reflectivity spectroscopy (DRS) and
four-wave mixing (FWM) experiments were performed simultaneously to study the
initial temporal dynamics of the exciton line-shapes in GaN epilayers. Beats
between the A-B excitons were found \textit{only for positive time delay} in
both PP and FWM experiments. The rise time at negative time delay for the
differential reflection spectra was much slower than the FWM signal or PP
differential transmission spectroscopy (DTS) at the exciton resonance. A
numerical solution of a six band semiconductor Bloch equation model including
nonlinearities at the Hartree-Fock level shows that this slow rise in the DRS
results from excitation induced dephasing (EID), that is, the strong density
dependence of the dephasing time which changes with the laser excitation
energy.Comment: 8 figure
Self-consistent model of ultracold atomic collisions and Feshbach resonances in tight harmonic traps
We consider the problem of cold atomic collisions in tight traps, where the
absolute scattering length may be larger than the trap size. As long as the
size of the trap ground state is larger than a characteristic length of the van
der Waals potential, the energy eigenvalues can be computed self-consistently
from the scattering amplitude for untrapped atoms. By comparing with the exact
numerical eigenvalues of the trapping plus interatomic potentials, we verify
that our model gives accurate eigenvalues up to milliKelvin energies for single
channel s-wave scattering of Na atoms in an isotropic harmonic trap,
even when outside the Wigner threshold regime. Our model works also for
multi-channel scattering, where the scattering length can be made large due to
a magnetically tunable Feshbach resonance.Comment: 7 pages, 4 figures (PostScript), submitted to Physical Review
Enhancement of spin Seebeck effect in Fe3O4/Pt thin films with α-Fe nanodroplets
In this study, we demonstrate an enhancement of the measured spin Seebeck coefficient in Fe3O4/Pt bilayer films due to an increase in Fe nanodroplets formed by pulsed laser deposition. Four bilayer films were deposited at the same time from a highly textured target, resulting in a general increase in droplet formation that was confirmed to be Fe rich by scanning electron microscope and transmission electron microscope-dispersive x-ray spectroscopy. Of these four films, there were two distinct groupings with differing density of α-Fe droplets, where the bilayer with higher droplet density exhibited a 64% increase in the measured spin Seebeck coefficient from 38 to 63 nVâm/W
Photoassociation spectroscopy of cold calcium atoms
Photoassociation spectroscopy experiments on 40Ca atoms close to the
dissociation limit 4s4s 1S0 - 4s4p 1P1 are presented. The vibronic spectrum was
measured for detunings of the photoassociation laser ranging from 0.6 GHz to 68
GHz with respect to the atomic resonance. In contrast to previous measurements
the rotational splitting of the vibrational lines was fully resolved. Full
quantum mechanical numerical simulations of the photoassociation spectrum were
performed which allowed us to put constraints on the possible range of the
calcium scattering length to between 50 a_0 and 300 a_0
High-precision calculations of van der Waals coefficients for heteronuclear alkali-metal dimers
Van der Waals coefficients for the heteronuclear alkali-metal dimers of Li,
Na, K, Rb, Cs, and Fr are calculated using relativistic ab initio methods
augmented by high-precision experimental data. We argue that the uncertainties
in the coefficients are unlikely to exceed about 1%.Comment: 11 pages, 2 figs, graphicx.st
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