9 research outputs found
Optogenetic activation of mGluR1 signaling in the cerebellum induces synaptic plasticity
Summary: Neuronal plasticity underlying cerebellar learning behavior is strongly associated with type 1 metabotropic glutamate receptor (mGluR1) signaling. Activation of mGluR1 leads to activation of the Gq/11 pathway, which is involved in inducing synaptic plasticity at the parallel fiber-Purkinje cell synapse (PF-PC) in form of long-term depression (LTD). To optogenetically modulate mGluR1 signaling we fused mouse melanopsin (OPN4) that activates the Gq/11 pathway to the C-termini of mGluR1 splice variants (OPN4-mGluR1a and OPN4-mGluR1b). Activation of both OPN4-mGluR1 variants showed robust Ca2+ increase in HEK cells and PCs of cerebellar slices. We provide the prove-of-concept approach to modulate synaptic plasticity via optogenetic activation of OPN4-mGluR1a inducing LTD at the PF-PC synapse in vitro. Moreover, we demonstrate that light activation of mGluR1a signaling pathway by OPN4-mGluR1a in PCs leads to an increase in intrinsic activity of PCs in vivo and improved cerebellum driven learning behavior
Preclinical and Clinical Evaluation of a Novel, Variable-View, Rigid Endoscope for Female Cystoscopy
Hydrodynamic performance of the flippers of large-bodied cetaceans in relation to locomotor ecology
Optogenetic activation of mGluR1 signaling in the cerebellum induces synaptic plasticity
Neuronal plasticity underlying cerebellar learning behavior is strongly associated with type 1 (mGluR1) signaling. Activation of mGluR1 leads to activation of the pathway, which is involved in inducing synaptic plasticity at the parallel fiber-Purkinje cell synapse (PF-PC) in form of long-term depression (LTD). To optogenetically modulate mGluR1 signaling we fused mouse (OPN4) that activates the pathway to the C-termini of mGluR1 splice variants (OPN4-mGluR1a and OPN4-mGluR1b). Activation of both OPN4-mGluR1 variants showed robust increase in HEK cells and PCs of cerebellar slices. We provide the prove-of-concept approach to modulate synaptic plasticity via activation of OPN4-mGluR1a inducing LTD at the PF-PC synapse . Moreover, we demonstrate that light activation of mGluR1a by OPN4-mGluR1a in PCs leads to an increase in intrinsic activity of PCs and improved cerebellum driven learning behavior