143 research outputs found
Distribution of the retrotransposable element 412 in Drosophila species
Copy numbers of sequences homologous to the Drosophila melanogaster retrotransposable element 412, their dis tribution between the chromosome arms and the chromocenter, and whether they contain full-size copies were
analyzed for 55 species of the Drosophila genus. Element 412 insertion sites were detected on the chromosome
arms of D. melanogaster, Drosophila simulans, and a few species of the obscura group, but the chromocenter was
labeled in almost all species. The presence of element 412 sequences in the majority of species shows that this
element has a long evolutionary history in Drosophilidae, although it may have recently invaded the chromosomes
in some species, such as D. simulans. Differences in copy number between species may be due to population size
or specific endogenous or environmental factors and may follow the worldwide invasion of the species. Putative
full-length copies were detected in the chromocenters of some species with no copies on the chromosome arms,
suggesting that the chromocenter may be a shelter for such copies and not only for deleted ones.info:eu-repo/semantics/publishedVersio
Phenetic distances in the Drosophila melanogaster-subgroup species and oviposition-site preference for food components
Oviposition-site preferences (O.S.P.) have been investigated in females of six sibling species of the Drosophila melanogaster subgroup. O.S.P. were determined for standard food components and yeast genotypes. Females of all species showed a strong preference for complete medium and avoidance of pure agar as an egg-deposition site.\ud
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Ecological trees of the species on the basis of rank correlations were constructed. In ‘no-choice’ situations they agree with phylogenetic trees obtained by different means but in ‘choice’ situations they do not agree too well.\ud
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All species showed a high egg production on live yeast compared with standard medium (with killed yeast) and D. erecta females demonstrated discrimination between yeast genotypes. Niche breadth calculated from survival on the sterol mutant yeasts correlated fairly well with phylogenetic trees
Stability of low back pain reporting over 8 years in a general population aged 40/41 years at base-line: data from three consecutive cross-sectional surveys
Individual courses of low back pain in adult Danes: a cohort study with 4-year and 8-year follow-up
Statistical Inference of Selection and Divergence from a Time-Dependent Poisson Random Field Model
We apply a recently developed time-dependent Poisson random field model to aligned DNA sequences from two related biological species to estimate selection coefficients and divergence time. We use Markov chain Monte Carlo methods to estimate species divergence time and selection coefficients for each locus. The model assumes that the selective effects of non-synonymous mutations are normally distributed across genetic loci but constant within loci, and synonymous mutations are selectively neutral. In contrast with previous models, we do not assume that the individual species are at population equilibrium after divergence. Using a data set of 91 genes in two Drosophila species, D. melanogaster and D. simulans, we estimate the species divergence time (or 1.68 million years, assuming the haploid effective population size years) and a mean selection coefficient per generation . Although the average selection coefficient is positive, the magnitude of the selection is quite small. Results from numerical simulations are also presented as an accuracy check for the time-dependent model
Duplication and Gene Conversion in the Drosophila melanogaster Genome
Using the genomic sequences of Drosophila melanogaster subgroup, the pattern of gene duplications was investigated with special attention to interlocus gene conversion. Our fine-scale analysis with careful visual inspections enabled accurate identification of a number of duplicated blocks (genomic regions). The orthologous parts of those duplicated blocks were also identified in the D. simulans and D. sechellia genomes, by which we were able to clearly classify the duplicated blocks into post- and pre-speciation blocks. We found 31 post-speciation duplicated genes, from which the rate of gene duplication (from one copy to two copies) is estimated to be 1.0×10−9 per single-copy gene per year. The role of interlocus gene conversion was observed in several respects in our data: (1) synonymous divergence between a duplicated pair is overall very low. Consequently, the gene duplication rate would be seriously overestimated by counting duplicated genes with low divergence; (2) the sizes of young duplicated blocks are generally large. We postulate that the degeneration of gene conversion around the edges could explain the shrinkage of “identifiable” duplicated regions; and (3) elevated paralogous divergence is observed around the edges in many duplicated blocks, supporting our gene conversion–degeneration model. Our analysis demonstrated that gene conversion between duplicated regions is a common and genome-wide phenomenon in the Drosophila genomes, and that its role should be especially significant in the early stages of duplicated genes. Based on a population genetic prediction, we applied a new genome-scan method to test for signatures of selection for neofunctionalization and found a strong signature in a pair of transporter genes
Reproductive Isolation Among Geographical Populations of Drosophila Bipectinata Duda (Diptera, Drosophilidae) with Recognition of Three Subspecies
Phenetic distances in the Drosophila melanogaster-subgroup species and oviposition-site preference for food components
Evolution of Sex-Specific Traits through Changes in HOX-Dependent doublesex Expression
Analysis in Drosophila suggests that evolutionary changes in the spatial regulation of the transcription factor doublesex play a key role in the origin, diversification, and loss of sex-specific structures
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