A Poroelastic Mixture Model of Mechanobiological Processes in Biomass Growth: Theory and Application to Tissue Engineering

In this article we propose a novel mathematical description of biomass growth that combines poroelastic theory of mixtures and cellular population models. The formulation, potentially applicable to general mechanobiological processes, is here used to study the engineered cultivation in bioreactors of articular chondrocytes, a process of Regenerative Medicine characterized by a complex interaction among spatial scales (from nanometers to centimeters), temporal scales (from seconds to weeks) and biophysical phenomena (fluid-controlled nutrient transport, delivery and consumption; mechanical deformation of a multiphase porous medium). The principal contribution of this research is the inclusion of the concept of cellular \u201cforce isotropy\u201d as one of the main factors influencing cellular activity. In this description, the induced cytoskeletal tensional states trigger signalling transduction cascades regulating functional cell behavior. This mechanims is modeled by a parameter which estimates the influence of local force isotropy by the norm of the deviatoric part of the total stress tensor. According to the value of the estimator, isotropic mechanical conditions are assumed to be the promoting factor of extracellular matrix production whereas anisotropic conditions are assumed to promote cell proliferation. The resulting mathematical formulation is a coupled system of nonlinear partial differential equations comprising: conservation laws for mass and linear momentum of the growing biomass; advection\u2013diffusion\u2013reaction laws for nutrient (oxygen) transport, delivery and consumption; and kinetic laws for cellular population dynamics. To develop a reliable computational tool for the simulation of the engineered tissue growth process the nonlinear differential problem is numerically solved by: (1) temporal semidiscretization; (2) linearization via a fixed-point map; and (3) finite element spatial approximation. The biophysical accuracy of the mechanobiological model is assessed in the analysis of a simplified 1D geometrical setting. Simulation results show that: (1) isotropic/anisotropic conditions are strongly influenced by both maximum cell specific growth rate and mechanical boundary conditions enforced at the interface between the biomass construct and the interstitial fluid; (2) experimentally measured features of cultivated articular chondrocytes, such as the early proliferation phase and the delayed extracellular matrix production, are well described by the computed spatial and temporal evolutions of cellular populations

Grid computing technologies for renewable electricity generator monitoring and control

In this paper we discuss the use of real-time Grid computing for the monitoring, control and simulation of renewable electricity generators and their associated electrical networks. We discuss briefly the architectural design of GRIDCC and how we have integrated a number of real (solar, CHP) and simulated conventional power generators into the GRIDCC environment. A local weather station has also been attached to an Instrument Manager to alert experts appropriately when the Solar Array is not generating. The customised remote control and monitoring environment (a virtual control room), distributed using a standard web server, is discussed

Target-Selective Drug Delivery through Liposomes Labeled with Oligobranched Neurotensin Peptides.

The structure and the in vitro behavior of liposomes filled with the cytotoxic drug doxorubicin (Doxo) and functionalized on the external surface with a branched moiety containing four copies of the 8-13 neurotensin (NT) peptide is reported. The new functionalized liposomes, DOPC-NT(4) Lys(C(18) )(2) , are obtained by co-aggregation of the DOPC phospholipid with a new synthetic amphiphilic molecule, NT(4) Lys(C(18) )(2) , which contains a lysine scaffold derivatized with a lipophilic moiety and a tetrabranched hydrophilic peptide, NT8-13, a neurotensin peptide fragment well known for its ability to mimic the neurotensin peptide in receptor binding ability. Dynamic light scattering measurements indicate a value for the hydrodynamic radius (RH) of 88.3±4.4 nm. The selective internalization and cytotoxicity of DOPC-NT(4) Lys(C(18) )(2) liposomes containing Doxo, as compared to pure DOPC liposomes, were tested in HT29 human colon adenocarcinoma and TE671 human rhabdomyosarcoma cells, both of which express neurotensin receptors. Peptide-functionalized liposomes show a clear advantage in comparison to pure DOPC liposomes with regard to drug internalization in both HT29 and TE671 tumor cells: FACS analysis indicates an increase in fluorescence signal of the NT(4) -liposomes, compared to the DOPC pure analogues, in both cell lines; cytotoxicity of DOPC-NT(4) Lys(C(18) )(2) -Doxo liposomes is increased four-fold with respect to DOPC-Doxo liposomes in both HT29 and TE671 cell lines. These effects could to be ascribed to the higher rate of internalization for DOPC-NT(4) Lys(C(18) )(2) -Doxo liposomes, due to stronger binding driven by a lower dissociation constant of the NT(4) -liposomes that bind the membrane onto a specific protein, in contrast to DOPC liposomes, which approach the plasma membrane unselectively

Host range of mammalian orthoreovirus type 3 widening to alpine chamois

Mammalian orthoreoviruses (MRV) type 3 have been recently identified in human and several animal hosts, highlighting the apparent lack of species barriers. Here we report the identification and genetic characterization of MRVs strains in alpine chamois, one of the most abundant wild ungulate in the Alps. Serological survey was also performed by MRV neutralization test in chamois population during five consecutive years (2008-2012). Three novel MRVs were isolated on cell culture from chamois lung tissues. No respiratory or other clinical symptoms neither lung macroscopic lesions were observed in the chamois population. MRV strains were classified as MRV-3 within the lineage III, based on S1 phylogeny, and were closely related to Italian strains identified in dog, bat and diarrheic pig. The full genome sequence was obtained by next-generation sequencing and phylogenetic analyses showed that other segments were more similar to MRVs of different geographic locations, serotypes and hosts, including human, highlighting genome reassortment and lack of host specific barriers. By using serum neutralization test, a high prevalence of MRV-3 antibodies was observed in chamois population throughout the monitored period, showing an endemic level of infection and suggesting a self-maintenance of MRV and/or a continuous spill-over of infection from other animal species

Epiphytic lichens of the sacred natural site "Bosco di Sant'Antonio" (Majella National Park - Abruzzo)

Sacred Natural Sites are relevant for biodiversity conservation, as in the case of forest sites that, across centuries, developed old growth structures and are now crucial for the conservation of epiphytic lichens and other specialized forest organisms. In this study, we investigated the epiphytic lichen flora of a small forest patch included in the Majella National Park (Abruzzo), whose old growth features and naturalness reflect its long lasting spiritual role that perfectly fits with the concept of Sacred Natural Site. Results revealed that the "Bosco di Sant'Antonio" hosts a rich and interesting epiphytic lichen flora, thus indicating the potential of this Sacred Natural Site for lichen conservation. Fifty-six species were found including two species newly recorded in Abruzzo, two red-listed species, and the sensitive species Lobaria pulmonaria. This study corroborates the hypothesis that sacred forest sites are relevant for the conservation of specialized epiphytic lichens. In particular, in the Italian forest landscape where old-growth stands are practically absent, sacred forest sites may provide unique old-growth structures and buffer anthropogenic disturbance

Do dogs and cats passively carry sars-cov-2 on hair and pads?

The epidemiological role of domestic animals in the spread and transmission of SARS-CoV-2 to humans has been investigated in recent reports, but some aspects need to be further clarified. To date, only in rare cases have dogs and cats living with COVID-19 patients been found to harbour SARS-CoV-2, with no evidence of pet-to-human transmission. The aim of the present study was to verify whether dogs and cats act as passive mechanical carriers of SARS-CoV-2 when they live in close contact with COVID-19 patients. Cutaneous and interdigital swabs collected from 48 dogs and 15 cats owned by COVID-19 patients were tested for SARS-CoV-2 by qRT-PCR. The time elapsed between owner swab positivity and sample collection from pets ranged from 1 to 72 days, with a median time of 23 days for dogs and 39 days for cats. All samples tested negative, suggesting that pets do not passively carry SARS-CoV-2 on their hair and pads, and thus they likely do not play an important role in the virus transmission to humans. This data may contribute to confirming that the direct contact with the hair and pads of pets does not represent a route for the transmission of SARS-CoV-2

Exploring patterns of beta-diversity to test the consistency of biogeographical boundaries: A case study across forest plant communities of Italy

Aim: To date, despite their great potential biogeographical regionalization models have been mostly developed on descriptive and empirical bases. This paper aims at applying the beta-diversity framework on a statistically representative data set to analytically test the consistency of the biogeographical regionalization of Italian forests. Location: Italy. Taxon: Vascular plants. Methods: Forest plant communities were surveyed in 804 plots made in a statistically representative sample of forest communities made by 201 sites of Italian forests across the three biogeographical regions of the country: Alpine, Continental, and Mediterranean. We conducted an ordination analysis and an analysis of beta-diversity, decomposing it into its turnover and nestedness components. Results: Our results provide only partial support to the consistency of the biogeographical regionalization of Italy. While the differences in forest plant communities support the distinction between the Alpine and the other two regions, differences between Continental and Mediterranean regions had lower statistical support. Pairwise beta-diversity and its turnover component are higher between- than within-biogeographical regions. This suggests that different regional species pools contribute to assembly of local communities and that spatial distance between-regions has a stronger effect than that within-regions. Main conclusions: Our findings confirm a biogeographical structure of the species pools that is captured by the biogeographical regionalization. However, nonsignificant differences between the Mediterranean and Continental biogeographical regions suggest that this biogeographical regionalization is not consistent for forest plant communities. Our results demonstrate that an analytical evaluation of species composition differences among regions using beta-diversity analysis is a promising approach for testing the consistency of biogeographical regionalization models. This approach is recommended to provide support to the biogeographical regionalization used in some environmental conservation polices adopted by EU

Relationships between CO2 soil degassing and regional/local fault systems in the Kiejo-Mbaka geothermal prospect (Tanzania)

The Kiejo-Mbaka geothermal prospect in the Mbeya region of SW Tanzania was surveyed for geothermal exploration in a recent study co-financed by the Ministry for Foreign Affairs in Iceland and by the Nordic Development Fund (NDF) and implemented by the Tanzania Geothermal Development Company Limited (TGDC). A multidisciplinary approach was adopted, including geological, geochemical and geophysical surveys. The prospect falls within the southern sector of the Rungwe Volcanic Province (RVP), which is situated at the triple junction of the Rukwa, Usangu and Karonga basins of the East Africa Rift System. Recent volcanism is concentrated in the northern sector of the RVP, whereas recent eruptions were sustained by small magma batches derived from deep sources in the prospect area. Local stratigraphy is mainly characterized by Pre-Cambrian rocks of the metamorphic-intrusive complex covered by Pleistocene volcanic products (basalts and ignimbrites) with a thickness never exceeding 200 m. Several fault systems are present, among which the most important ones trend NW-SE and N-S. The main tectonic feature is the NW-SE-trending Mbaka fault, which controls the local emergence of hot waters at Ilwalilo and Kilambo-Kajala (maximum discharge temperature of 64\ub0C), delimits to the W the Mbaka ridge, and is associated with a series of parallel structures extending in the plain. Both the gravimetric and the electromagnetic surveys concur in identifying the existence of a block corresponding to the above mentioned Mbaka ridge and characterized by a pronounced positive Bouguer anomaly and by high resistivity, due to the proximity of basement rocks. At Kiejo, Ikama, and Lufundo there are gas vents emitting CO2-rich gases, which are captured by drilled wells in the first two sites. Since the Kiejo-Mbaka prospect is classified as an extensional domain, in which flow-paths of geothermal fluids are fault-controlled, part of the geochemical survey was addressed to identify and define the relationships between fluid flow and structures at local scale, in selected hydrothermal areas including Kilambo-Kajala, Ilwalilo and Kiejo. Taking into account the regional fault distribution and preliminary results obtained during the field surveys, also others areas were included in the investigation (i.e. Lufundo, Itende and Kikusya). A total of 598 soil CO2 flux and temperature measurements (~1 m depth) were carried out. Total output was estimated and isoflux maps were elaborated for each investigated sector. In general, CO2 fluxes appear to be controlled by NW-SE and N-S trending faults and fractures. The former prevails at Kiejo, Kilambo-Kajala and Ilwalilo, which is not surprising for Kilambo-Kajala and Ilwalilo, since the hot springs are positioned along the Mbaka fault. In contrast, the N-S trend dominates at Lufundo