Radioanalitikai eljárás kifejlesztáse a thrittene elnvezésű peptid kvantitatív meghatározására és a módszer gyakorlati alkalmazása

Munkacsoportunk, amely a táplálkozás, obezitás és diabetes témakörben végez tudományos munkát, fontosnak tartotta egy érzékeny és specifikus thrittene RIA módszer kifejlesztését, amelynek segítségével vizsgálni tudjuk apeptidhormon biológiai szerepét.BscBiológiag

Comparative distribution of somatostatin and thrittene bioactive peptides in the central nervous system of rat measured by radioimmunoassay

In the present paper the development and application of a novel somatostatin RIA are described. 125I-labeling of Tyr(0)-somatostatin-14 (tyrosin(0)-somatostatin-14) was performed by the iodogen-method and the mono-iodinated peptide was sepd. by reversed-phase HPLC. As a practical application of the novel RIA, the present work measured the somatostatin concn. parallel with the detn. of thrittene content in different rat brain areas. The highest somatostatin concns. were detected in the cerebellum. The thrittene immunoreactivity in the same brain parts showed a similar tendency. In the present study concluded that thrittene is not created via a specific biosynthetical route, but from the pre-prosomatostatin. [on SciFinder(R)

Examination of PACAP-Like Immunoreactivity in Urogenital Tumor Samples.

Numerous studies investigated the localization of pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptors in different tumors and described the effects of analogs on tumor growth to show its potential role in oncogenesis. Recently, our research group has found significantly lower levels of PACAP27-like immunorreactivity (LI) and PACAP38-LI in different human samples of primary small cell lung cancer and colon cancer compared to normal healthy tissues. There are only few human studies showing the presence of PACAP and its receptors in urogenital tumors; therefore, the aim of the present study was to compare PACAP-LI in different healthy and pathological human samples from urogenital organs (kidney, urinary bladder, prostate, testis) with radioimmunoassay (RIA) method. Similar to our earlier observations, the PACAP27-LI was significantly lower compared to PACAP38-LI in all samples. We did not find significant alterations in PACAP-LI between healthy and tumoral samples from the urinary bladder and testis. On the other hand, we found significantly lower PACAP38-LI level in kidney tumors compared with healthy tissue samples, and we showed higher PACAP27-LI in prostatic cancer compared to samples from benign prostatic hyperplasia. These data indicate that PACAP levels of different tissue samples are altered under pathological conditions suggesting a potential role of PACAP in the development of different urogenital tumors

THE ROLE OF MELANIN CONCENTRATING HORMONE (MCH) IN THE PATHOMECHANISM OF CHILDHOOD OBESITY AND DIABETES MELLITUS

Our previous preclinical experiments proved that various tissues contains a great concentration of MCH and the diet induced release of MCH results in significantly increased plasma levels of the hormone. These findings concluded that MCH is not only a neurotransmitter in the central nervous system but a gastrointestinal hormone that controls the food intake and play an important role in digestion. It is presumed to have effects on some clinical conditions such as abnormal obesity or abnormal tenderness. The objective of our study was to evaluate the MCH as a possible prognostic biomarker in the development of obesity