1 research outputs found
Production of enzymes from Lichtheimia ramosa using Brazilian savannah fruit wastes as substrate on solid state bioprocessess
Background: Enzyme production by solid state bioprocess (SSB) using
residues as substrate for microorganisms is an alternative for costs
reduction and to avoid their disposal into environment. The aim of this
work was to evaluate the physiology of the fungus Lichtheimia ramosa
in terms of microbial growth and production of amylases,
\u3b2-glucosidases, carboxymethylcellulase (CMCase), and xylanases,
via SSB, utilizing wastes of the Brazilian savannah fruits bocaiuva (
Acrocomia aculeata ), guavira ( Campomanesia pubescens) and pequi (
Caryocar brasiliense ) as substrate at different temperatures (25, 30,
and 35oC) during 168 hrs. Results: Samples were taken every 24 hrs,
which resulted in 8-points kinetic experiments to determine
microbiological and enzymatic contents. The best substrate for
\u3b2-glucosidase activity was pequi waste after 48 hrs at 30oC (0.061
U/mL). For amylase activity, bocaiuva presented itself as the best
substrate after 96 hrs at 30oC (0.925 U/mL). CMCase activity was higher
in guavira waste after 96 hrs at 35oC (0.787 U/mL). However, the
activity was more expressive for xylanase in substrate composed of
bocaiuva residue after 144 hrs at 35oC (1.802 U/mL). Conclusions: It
was concluded that best growth condition for L. ramosa is at 35oC for
all substrates and that xylanase is the enzyme with more potential in
SSB, considering the studied Brazilian savannah fruit wastes