Factors influencing the potency of marbofloxacin for pig pneumonia pathogens Actinobacillus pleuropneumoniae and Pasteurella multocida

For the pig respiratory tract pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida, Minimum Inhibitory Concentration (MIC) of marbofloxacin was determined in recommended broths and pig serum at three inoculum strengths. MICs in both growth matrices increased progressively from low, through medium to high starting inoculum counts, 104, 106 and 108 CFU/mL, respectively. P. multocida MIC ratios for high:low inocula were 14:4:1 for broth and 28.2:1 for serum. Corresponding MIC ratios for A. pleuropneumoniae were lower, 4.1:1 (broth) and 9.2:1 (serum). MIC high:low ratios were therefore both growth matrix and bacterial species dependent. The effect of alterations to the chemical composition of broths and serum on MIC were also investigated. Neither adjusting broth or serum pH in six increments over the range 7.0 to 8.0 nor increasing calcium and magnesium concentrations of broth in seven incremental steps significantly affected MICs for either organism. In time-kill studies, the killing action of marbofloxacin had the characteristics of concentration dependency against both organisms in both growth matrices. It is concluded that MIC and time-kill data for marbofloxacin, generated in serum, might be preferable to broth data, for predicting dosages of marbofloxacin for clinical use

Potency of marbofloxacin for pig pneumonia pathogens Actinobacillus pleuropneumoniae and Pasteurella multocida: Comparison of growth media

Pharmacodynamic properties of marbofloxacin were established for six isolates each of the pig respiratory tract pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Three in vitro indices of potency were determined; Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Mutant Prevention Concentration (MPC). For MIC determination Clinical Laboratory Standards Institute guidelines were modified in three respects: (1) comparison was made between two growth media, an artificial broth and pig serum; (2) a high inoculum count was used to simulate heavy clinical bacteriological loads; and (3) five overlapping sets of two-fold dilutions were used to improve accuracy of determinations. Similar methods were used for MBC and MPC estimations. MIC and MPC serum:broth ratios for A. pleuropneumoniae were 0.79:1 and 0.99:1, respectively, and corresponding values for P. multocida were 1.12:1 and 1.32:1. Serum protein binding of marbofloxacin was 49%, so that fraction unbound (fu) serum MIC values were significantly lower than those predicted by correction for protein binding; fu serum:broth MIC ratios were 0.40:1 (A. pleuropneumoniae) and 0.50:1 (P. multocida). For broth, MPC:MIC ratios were 13.7:1 (A. pleuropneumoniae) and 14.2:1 (P. multocida). Corresponding ratios for serum were similar, 17.2:1 and 18.8:1, respectively. It is suggested that, for dose prediction purposes, serum data might be preferable to potency indices measured in broths

Cancer and communication: similarities and differences of men with cancer from six different ethnic groups.

Objective To report the communication aspects of a pilot study, which explored the cancer meanings and experiences of six men with cancer and their significant others from different ethnic groups. Design Case study design using the principles of phenomenology. In-depth semi-structured individual interviews with men with cancer and their wives were conducted. Setting London, UK. Interviews took place in participants’ own homes. Respondents Six men with cancer and their wives from different ethnic groups. Intervention The pilot study explored the participants’ experiences and meanings of cancer. However, this paper only deals with the communication aspects of the findings. Results Ten themes emerged from the comparative analysis of the study’s data, with communication as a cross-cutting theme. Further analysis of this theme revealed similarities and differences of the participants’ experiences of communicating with health professionals; families or friends; and God/Allah. In addition, similarities and differences of communicating meanings of cancer in different cultures were revealed. Conclusion The findings revealed similarities in the way that men from these six cultures communicate with health professionals and their families following a diagnosis of cancer, and differences in how they communicated with God/Allah, which depended on their religious beliefs and practices

Developing culturally competent researchers

Background Whilst we live in multi-cultural societies most health researchers tend to take the cultural perspective of the majority ethnic group at the expense of the perspective of minority ethnic groups. Aim This paper discusses the need for the development of culturally competent health researchers in all areas of research and proposes a model for the achievement of this. Design A snapshot review of research textbooks used in nursing curricula was conducted to identify whether culturally competent research was being promoted. Results The review found that whilst a few textbooks touched on ethnicity, race and culture, none of them addressed the issue of culturally competence. Subsequently the authors adapted their existing model of culturally competent health care practice, and in this paper they propose it as a model for the development of culturally competent researchers. Discussion The model put forward by the authors consists of four concepts: cultural awareness, cultural knowledge, cultural sensitivity and cultural competence. A culturally competent researcher is one who is able to apply the related skills and knowledge in project design, data collection, analysis, report writing and dissemination. Furthermore, the authors identify two layers of cultural competence, those of culture-generic (knowledge and skills that are applicable across ethnic groups) and culture-specific competence (knowledge and skills that relate to a particular ethnic group). The relationship between these two layers is a dynamic and spiralling process as illustrated by the model. Conclusion Current health policy in many developed countries focuses on inequalities of health and managing diversity, including ethnicity. Thus the authors conclude that the development of culturally competent researchers will lead to both valid research and culturally competent practice by health care professionals

Improved AURA k-Nearest Neighbour approach

The k-Nearest Neighbour (kNN) approach is a widely-used technique for pattern classification. Ranked distance measurements to a known sample set determine the classification of unknown samples. Though effective, kNN, like most classification methods does not scale well with increased sample size. This is due to their being a relationship between the unknown query and every other sample in the data space. In order to make this operation scalable, we apply AURA to the kNN problem. AURA is a highly-scalable associative-memory based binary neural-network intended for high-speed approximate search and match operations on large unstructured datasets. Previous work has seen AURA methods applied to this problem as a scalable, but approximate kNN classifier. This paper continues this work by using AURA in conjunction with kernel-based input vectors, in order to create a fast scalable kNN classifier, whilst improving recall accuracy to levels similar to standard kNN implementations

Disorder-induced critical exponents near a ferromagnetic quantum critical point in Mn1−xCrxSi

We report the observation of critical behavior in Mn1−xCrxSi (0≤x≤1) close to a T = 0 K quantum critical point, consistent with the Belitz-Kirkpatrick-Vojta (BKV) theory of disordered metallic ferromagnets. The critical exponents are in good agreement with the theoretical predictions of the BKV theory in the preasymptotic limit. A non-Fermi liquid-like behavior is seen down to 200 mK in the transport and thermodynamic properties around the critical concentration xC = 0.2. Quantum criticality and self-consistency of the exponents is further confirmed using a scaling analysis of the magnetization and heat capacity data. A recovery to Fermi liquid-like behavior is displayed on moving away from the critical composition, as well as with the application of a magnetic field

Optimization of multivariate analysis for IACT stereoscopic systems

Multivariate methods have been recently introduced and successfully applied for the discrimination of signal from background in the selection of genuine very-high energy gamma-ray events with the H.E.S.S. Imaging Atmospheric Cerenkov Telescope. The complementary performance of three independent reconstruction methods developed for the H.E.S.S. data analysis, namely Hillas, model and 3D-model suggests the optimization of their combination through the application of a resulting efficient multivariate estimator. In this work the boosted decision tree method is proposed leading to a significant increase in the signal over background ratio compared to the standard approaches. The improved sensitivity is also demonstrated through a comparative analysis of a set of benchmark astrophysical sources.Comment: 10 pages, 8 figures, 3 tables, accepted for publication in Astroparticle Physic

Assessing the performance of protective winter covers for outdoor marble statuary: pilot investigation

Outdoor statuary in gardens and parks in temperate climates has a tradition of being covered during the winter, to protect against external conditions. There has been little scientific study of the environmental protection that different types of covers provide. This paper examines environmental conditions provided by a range of covers used to protect marble statuary at three sites in the UK. The protection required depends upon the condition of the marble. Although statues closely wrapped and with a layer of insulation provide good protection, this needs to be considered against the potential physical damage of close wrapping a fragile deteriorated surface