5 research outputs found
<span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-US">Efficacy of different extraction procedures on antibacterial activity of stem bark of <i style="mso-bidi-font-style:normal">Butea monosperma</i> (Lam.) Kuntze</span>
551-556The search for new antimicrobials of natural origin
derived from plants is still very important at present. Over the decades it has
been observed that selection of solvents is critical for the extraction of
active compounds from plants. In this study, three solvents namely, hexane,
chloroform and methanol were employed to extract the active constituents from
the stem bark of Butea monosperma (Lam.) Kuntze. High performance thin
layer chromatography (HPTLC) was used to separate and establish the active
constituents from the bark of the plant. Active constituent were tested for their
antibacterial activity against five Gram positive bacteria (Bacillus subtilis, <i style="mso-bidi-font-style:
normal">Enterococcus faecalis, Micrococcus
luteus, Staphylococcus aureus and
S. epidermidis) and seven Gram
negative (Aeromonas hydrophila, Alcaligenes faecalis, Enterobacter aerogenes, Escherichia coli, <i style="mso-bidi-font-style:
normal">Klebsiella pneumoniae, Pseudomonas
aeruginosa and Salmonella typhimurium) bacteria using
agar well diffusion assay. Relatively low minimum inhibitory concentrations
(MIC) were obtained for hexane and methanol extracts compared to chloroform
extracts. Our findings revealed that hexane and methanol could be used as best
solvents for extraction of antimicrobial compounds from <i style="mso-bidi-font-style:
normal">B. monosperma bark.
</span
Antibacterial, Antioxidant, Collagenase inhibition, Urease inhibition activities and Chemical composition of the Essential oil and extracts of Cinnamomum zeylanicum bark.
Significant biological activities of bacterial infection like antibacterial, antioxidant, urease and collagenase inhibitory activities of the essential oil and various extracts of Cinnamomum zeylanicum were determined. The in vitro antibacterial activity against nine epidermal bacteria and 11 MRSA isolates was conducted. The essential oil of C. zeylanicum exhibited the activity against all bacteria with the Minimum Inhibitory Concentration values (MIC) ranging from 0.15 to 2.5 µl. The extracts showed declined antibacterial activity. The scanning electron microscopic (SEM) studies demonstrated potential detrimental effect of the essential oil on the morphology of tested bacteria. Antioxidant activity of all the samples was also determined and 20% activity was observed in essential oil. Collagenase inhibitory potential was upto 25% and 30% in aqueous and methanol extracts respectively while upto 35% in essential oil. None of the samples showed urease inhibitory activity. The chemical composition of hydrodistilled essential oil of C. zeylanicum was also analysed by GC-MS. A total of 13 compounds representing 100% of the oil were identified. Therefore, due to different chemical components present in the cinnamon essential oil, further investigation concerned with studied activities of this essential oil to replace the medicines or as supplement in diseases, is recommended
Antibacterial, Antioxidant, Collagenase inhibition, Urease inhibition activities and Chemical composition of the Essential oil and extracts of Cinnamomum zeylanicum bark.
Significant biological activities of bacterial infection like antibacterial, antioxidant, urease and collagenase inhibitory activities of the essential oil and various extracts of Cinnamomum zeylanicum were determined. The in vitro antibacterial activity against nine epidermal bacteria and 11 MRSA isolates was conducted. The essential oil of C. zeylanicum exhibited the activity against all bacteria with the Minimum Inhibitory Concentration values (MIC) ranging from 0.15 to 2.5 µl. The extracts showed declined antibacterial activity. The scanning electron microscopic (SEM) studies demonstrated potential detrimental effect of the essential oil on the morphology of tested bacteria. Antioxidant activity of all the samples was also determined and 20% activity was observed in essential oil. Collagenase inhibitory potential was upto 25% and 30% in aqueous and methanol extracts respectively while upto 35% in essential oil. None of the samples showed urease inhibitory activity. The chemical composition of hydrodistilled essential oil of C. zeylanicum was also analysed by GC-MS. A total of 13 compounds representing 100% of the oil were identified. Therefore, due to different chemical components present in the cinnamon essential oil, further investigation concerned with studied activities of this essential oil to replace the medicines or as supplement in diseases, is recommended
Free radical scavenging activity, phenolic contents and Phytochemical analysis of seeds of trigonella foenum graecum
The search for sources of potent antioxidants of natural origin derived from plants is very important in the wake of decreasing resistance of human beings to various diseases. In the present study, antioxidant activity, phenolic contents and phytochemical profile of various extracts (methanol, chloroform, ethyl acetate and hexane) of seeds of Trigonella foenum graecum (fenugreek) were investigated. Ethyl acetate extract (100 μg/ml) showed highest inhibitory potential with application of DPPH scavenging (69.70%) and chelating power assay (63.44%). The differences in antioxidant activity of extracts are attributed to the presence of various phytochemicals. The IC50 values of different extracts were also calculated. There was found a positive correlation (R ≥ 0.895) between the total phenolic content and the antioxidant activity of extracts. The phytochemical analysis revealed the presence of alkaloids, flavonoids, saponins, phenols and tannins. Results from different parameters were in agreement with each other. The results reveal that all extracts of the fenugreek exhibit antioxidant activity. These findings suggest that the fenugreek extracts could act as potent source of antioxidants