Form Factors for Exclusive Semileptonic BB--Decays

We developed the new parton model approach for exclusive semileptonic decays of $B$-meson to $D,~D^*$ by extending the inclusive parton model, and by combining with the results of the HQET, motivated by Drell-Yan process. Without the nearest pole dominance ans\"atze, we {\bf derived} the dependences of hadronic form factors on $q^2$. We also calculated numerically the slope of the Isgur-Wise function, which is consistent with the experimental results.Comment: 20 pages, RevTex, 2 ps figure files(uuencoded in seperate file

High energy-charged cell factory for heterologous protein synthesis

Overexpression of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) under glycolytic conditions enables Escherichia coli to maintain a greater intracellular ATP concentration and, consequently, to up-regulate genes for amino acid and nucleotide biosynthesis. To investigate the effect of a high intracellular ATP concentration on heterologous protein synthesis, we studied the expression of a foreign gene product, enhanced green fluorescence protein (eGFP), under control of the T7 promoter in E. coli BL21(DE3) strain overexpressing PCK. This strain was able to maintain twice as much intracellular ATP and to express two times more foreign protein than the control strain. These results indicate that a high energy-charged cell can be beneficial as a protein-synthesizing cell factory. The potential uses of such a cell factory are discussed