Effects of the STEP parent education program on parenting styles and reported child behavior problems

Three groups of parents were given a nine week parent education course using Dinkmeyer and McKay\u27s Systematic Training for Effective Parenting. Three control groups were given no training. Pretest and posttest data were obtained from all six groups to test the hypotheses: Training with the STEP program will change parent attitudes toward a less authoritarian parenting style, and these changes will result in fewer problems reported by the parents in child behavior. Using Ernhart and Loevinger\u27s Authoritarian Ideology Scale no significant differences in authoritarianism were found after training [F(1,5) = 2.45, p = .123]. Using McKay\u27s Adlerian Parental Assessment of Child Behavior Scale significant differences were found [F(1,5) = 7.41, p = .009]. Implications for parent education are discussed

Fatherhood and Psychobiology in the Philippines: Perspectives on Joint Profiles and Longitudinal Changes of Fathers’ Estradiol and Testosterone

Objectives: Research on the psychobiology of partnering and fathering has focused on testosterone (T), oxytocin, and prolactin (PRL) as mechanisms that potentially mediate life history trade-offs related to those roles. Less is known about other hormones that might be responsive to life history transitions and implicated in fathering, such as estradiol (E2). We examined how E2 changed during the transition to marriage and fatherhood, its correlation with fathers’ caregiving, and its joint within-individual production with other hormones (T, PRL). Methods: Data were collected from a total of 913 Filipino men (aged 25.9 years ± 0.3 SD at follow-up) enrolled in a longitudinal cohort study. Morning saliva samples collected at baseline (2005) and follow-up (2009) were assayed for T and E2 (n = 329), dried blood spots from baseline were assayed for PRL. Fathers reported on caregiving in 2009. Results: When compared with men who remained single non-fathers over the study period, men who became married residential fathers experienced larger declines in E2. This effect was non-significant when we controlled for longitudinal changes in T. E2 was not significantly related to fathers’ caregiving, controlling for T. In cross-sectional analyses for PRL, T, and E2, married residential fathers exhibited within-individual profiles of reduced T and elevated PRL, whereas single non-fathers exhibited the opposite profile of elevated T and reduced PRL. Conclusions: Our findings point to the need for future research to consider the mutually regulatory dynamics and/or combinatorial implications of multiple physiological axes acting within individuals to underpin life history trade-offs and behavioral strategies

The Roles of Parents in Shaping Fathering Across Generations in Cebu, Philippines

Objective: This study examined how parental caregiving and parent–child closeness are associated with future fathering among 335 Filipino men who are participants in a long-running birth cohort study. Background Few studies have multidecade longitudinal data to test the pathways through which parenting is transmitted across generations, with most relevant research conducted in the United States, Europe, and other similar settings. The roles of mothers and fathers in shaping their sons’ future parenting is particularly understudied despite fathers having the potential to positively influence child health and development. Method: Participants’ mothers (Generation 1 [G1]) reported on caregiving during Generation 2 (G2) participants’ early life, and the G2 males reported parent–child closeness during adolescence. G2 fathers reported on their own child-care involvement and the salience of care- giving to their parenting identity. We tested whether parent–child closeness moderated the effect of early-life care to predict later-life fathering. Results: G1-G2 closeness moderated the association between G1 parents’ caregiving and G2 fathers’ parenting identity (for both G1 parents) and caregiving time (for G1 fathers only). When the G1-G2 mother–son relationship was not close, there was a negative correlation between G1 maternal care and G2 fathers’ caregiving identity. For G2 men who were close to their fathers, there were positive associations between G1 paternal care and G2 fathers’ caregiving identity and time, respectively. Among G2 men who were not close to their fathers, the slopes relating G1 paternal care to G2 fathers’ care- giving identity and time, respectively, were negative. Conclusion: These findings reflect that developmental experiences with both mothers and fathers are predictive of men’s identity as parents in adulthood and that closeness between fathers and sons moderates whether sons’ paternal care tends to emulate or diverge from their fathers’ caregiving patterns

Investigating moderators of daily marital to parent–child spillover: Individual and family systems approaches

Objective: We tested whether cognitive reappraisal and coparenting quality moderate marital to parent–child spillover in mothers and fathers. Background: The influence of marital relationship quality on parent–child relationships, referred to as the spillover effect, is well documented. Factors that may attenuate the occurrence of spillover, however, remain unclear. Cognitive reappraisal, an emotion regulation strategy that promotes the reframing of emotional situations as neutral or positive, and coparenting—the intermediate subsystem between the marital and parent–child relationships—may buffer the effects of marital to parent–child spillover. Method: Using daily diary data from mother–father couples (N = 96) of young children (Mage = 3.22 years), we investigated coparenting quality and cognitive reappraisal as moderators of marital and parent–child spillover within and between days. Results: Dyadic multilevel models revealed within-day spillover of marital emotional climate and parent–child emotional climate for both mothers and fathers. Whereas cognitive reappraisal moderated spillover for fathers, no significant moderators emerged for mothers. Fathers also experienced next-day associations between marital emotional climate and parent–child emotional climate the following day, whereas mothers did not. Coparenting quality accounted for next-day associations between fathers’ marital emotional climate and parent–child climate. Conclusion: Overall, our results evince that although spillover can be attenuated by both cognitive reappraisal and coparenting quality for fathers, the same is not true for mothers. Implications: These results signify the importance of considering mother and father differences in empirical investigations of spillover effects and processes within the family system, and the clinical implications recommended to marriage and family therapists

Impact of accelerometer data processing decisions on the sample size, wear time and physical activity level of a large cohort study

Background: Accelerometers objectively assess physical activity (PA) and are currently used in several large-scale epidemiological studies, but there is no consensus for processing the data. This study compared the impact of wear-time assessment methods and using either vertical (V)-axis or vector magnitude (VM) cut-points on accelerometer output. Methods: Participants (7,650 women, mean age 71.4 y) were mailed an accelerometer (ActiGraph GT3X+), instructed to wear it for 7 days, record dates and times the monitor was worn on a log, and return the monitor and log via mail. Data were processed using three wear-time methods (logs, Troiano or Choi algorithms) and V-axis or VM cut-points. Results: Using algorithms alone resulted in "mail-days" incorrectly identified as "wear-days" (27-79% of subjects had >7-days of valid data). Using only dates from the log and the Choi algorithm yielded: 1) larger samples with valid data than using log dates and times, 2) similar wear-times as using log dates and times, 3) more wear-time (V, 48.1 min more; VM, 29.5 min more) than only log dates and Troiano algorithm. Wear-time algorithm impacted sedentary time (~30-60 min lower for Troiano vs. Choi) but not moderate-to-vigorous (MV) PA time. Using V-axis cut-points yielded ~60 min more sedentary time and ~10 min less MVPA time than using VM cut-points. Conclusions: Combining log-dates and the Choi algorithm was optimal, minimizing missing data and researcher burden. Estimates of time in physical activity and sedentary behavior are not directly comparable between V-axis and VM cut-points. These findings will inform consensus development for accelerometer data processing in ongoing epidemiologic studies. Electronic supplementary material The online version of this article (doi:10.1186/1471-2458-14-1210) contains supplementary material, which is available to authorized users

Endothelial toll-like receptor 4 maintains lung integrity via epigenetic suppression of p16INK4a.

We previously reported that the canonical innate immune receptor toll-like receptor 4 (TLR4) is critical in maintaining lung integrity. However, the molecular mechanisms via which TLR4 mediates its effect remained unclear. In the present study, we identified distinct contributions of lung endothelial cells (Ec) and epithelial cells TLR4 to pulmonary homeostasis using genetic-specific, lung- and cell-targeted in vivo methods. Emphysema was significantly prevented via the reconstituting of human TLR4 expression in the lung Ec of TLR4-/- mice. Lung Ec-silencing of TLR4 in wild-type mice induced emphysema, highlighting the specific and distinct role of Ec-expressed TLR4 in maintaining lung integrity. We also identified a previously unrecognized role of TLR4 in preventing expression of p16INK4a , a senescence-associated gene. Lung Ec-p16INK4a -silencing prevented TLR4-/- induced emphysema, revealing a new functional role for p16INK4a in lungs. TLR4 suppressed endogenous p16INK4a expression via HDAC2-mediated deacetylation of histone H4. These findings suggest a novel role for TLR4 in maintaining of lung homeostasis via epigenetic regulation of senescence-related gene expression

Transcriptional regulation of FoxO3 gene by glucocorticoids in murine myotubes.

Glucocorticoids and FoxO3 exert similar metabolic effects in skeletal muscle. FoxO3 gene expression was increased by dexamethasone (Dex), a synthetic glucocorticoid, both in vitro and in vivo. In C2C12 myotubes the increased expression is due to, at least in part, the elevated rate of FoxO3 gene transcription. In the mouse FoxO3 gene, we identified three glucocorticoid receptor (GR) binding regions (GBRs): one being upstream of the transcription start site, -17kbGBR; and two in introns, +45kbGBR and +71kbGBR. Together, these three GBRs contain four 15-bp glucocorticoid response elements (GREs). Micrococcal nuclease (MNase) assay revealed that Dex treatment increased the sensitivity to MNase in the GRE of +45kbGBR and +71kbGBR upon 30- and 60-min Dex treatment, respectively. Conversely, Dex treatment did not affect the chromatin structure near the -17kbGBR, in which the GRE is located in the linker region. Dex treatment also increased histone H3 and/or H4 acetylation in genomic regions near all three GBRs. Moreover, using chromatin conformation capture (3C) assay, we showed that Dex treatment increased the interaction between the -17kbGBR and two genomic regions: one located around +500 bp and the other around +73 kb. Finally, the transcriptional coregulator p300 was recruited to all three GBRs upon Dex treatment. The reduction of p300 expression decreased FoxO3 gene expression and Dex-stimulated interaction between distinct genomic regions of FoxO3 gene identified by 3C. Overall, our results demonstrate that glucocorticoids activated FoxO3 gene transcription through multiple GREs by chromatin structural change and DNA looping

Exploring the Links between Early Life and Young Adulthood Social Experiences and Men’s Later Life Psychobiology as Fathers

Early life cues of environmental harshness and unpredictability have been hypothesized to influence within-species variation in the timing of life history transitions and the dynamics of reproductive strategies, such as investments in mating and parenting. It is also believed that adolescence is an influential developmental period for male reproductive strategies, with those who achieve greater social and sexual success during that period maintaining faster life history strategies into adulthood. If correct, such early life and post-pubertal experiences could also help shape the psychobiological pathways that mediate reproductive strategies, including the well-documented physiological shifts that occur when some men become parents. Drawing on a large sample of Filipino men (n = 417), we evaluate whether men who experienced cues of harshness or unpredictability in childhood or have earlier ages at sexual debut have elevated testosterone (T) as fathers. We also test whether males who experienced a combination of early life experiences of harshness or unpredictability and had earlier ages of sexual debut during adolescence had the most elevated T as fathers. We found that fathers who experienced early life harshness and who engaged in sex at an earlier age had elevated waking T. Among men transitioning to fatherhood across the 4.5-year follow-up period of this study, those who experienced unpredictability and who engaged in sex at an earlier age showed attenuated declines in waking T between baseline and follow-up. Complementing these findings, we found that fathers who first engaged in sex at later ages had greater acute declines in T when they played with their toddlers. We suggest that these patterns could reflect programming effects of sociosexual experiences during the years following the marked biological transitions that accompany puberty, which occur along with the better-studied effects of earlier life exposures to stressors. Overall, our results support the hypothesis that early life circumstances and social and sexual experiences, from early life to young adulthood, help calibrate physiological axes as key mechanisms coordinating dynamic life history strategies

Sociosexuality, testosterone, and life history status: Prospective associations and longitudinal changes among men in Cebu, Philippines

Sociosexuality is defined as an individual\u27s interest in uncommitted sexual activity and can be measured in terms of both psychological orientations and behavioral expression. In socio-ecological contexts in which adults monogamously partner and cooperate to raise children, individuals with unrestricted sociosexuality are likely to prioritize mating/competition over committed partnering and parenting. Given the importance of mother-father cooperation in the evolutionary past, humans may have the capacity to facultatively and opportunistically downregulate sociosexuality to focus on priorities related to invested partnering and parenting. To date, no prior studies have used longitudinal data to track within-individuals changes in sociosexuality as it relates to such life history transitions. Given the lack of prior longitudinal research in this area, it is likewise unknown what physiological mechanisms might mediate within-individual changes in sociosexuality through time but testosterone is a plausible candidate. To explore these questions, we drew on a large, long-running study of Filipino men (n=288), who were single non-fathers at 25.9 years of age and were followed up 4–5 years later. We found that men with more unrestricted sociosexuality at baseline were more likely to experience relationship dissolution by follow-up, consistent with past work. Compared to men who remained single non-fathers at follow-up, men who became married residential fathers showed shifts towards more restricted global sociosexuality as well as sociosexual behavior. Relative to their own baseline values, married residential fathers also had more restricted sociosexuality in all domains at follow-up. They were the only group for whom this was found. We found theoretically-consistent but modest support for positive correlations between men\u27s testosterone and their sociosexuality, but no evidence that the two change in tandem together through time. Our results suggest that some amount of between-individual differences in sociosexuality are not stable and can facultatively shift alongside other aspects of male reproductive effort

Effects of bone marrow‐derived mesenchymal stromal cells on gene expression in human alveolar type II cells exposed to TNF‐α, IL‐1β, and IFN‐γ

The acute respiratory distress syndrome (ARDS) is common in critically ill patients and has a high mortality rate. Mesenchymal stromal cells (MSCs) have demonstrated therapeutic potential in animal models of ARDS, and their benefits occur in part through interactions with alveolar type II (ATII) cells. However, the effects that MSCs have on human ATII cells have not been well studied. Using previously published microarray data, we performed genome‐wide differential gene expression analyses of human ATII cells that were (1) unstimulated, (2) exposed to proinflammatory cytokines (CytoMix), or (3) exposed to proinflammatory cytokines plus MSCs. Findings were validated by qPCR. Alveolar type II cells differentially expressed hundreds of genes when exposed either to proinflammatory cytokines or to proinflammatory cytokines plus MSCs. Stimulation with proinflammatory cytokines increased expression of inflammatory genes and downregulated genes related to surfactant function and alveolar fluid clearance. Some of these changes, including expression of some cytokines and genes related to surfactant, were reversed by exposure to MSCs. In addition, MSCs induced upregulation of other potentially beneficial genes, such as those related to extracellular matrix remodeling. We confirmed several of these gene expression changes by qPCR. Thus, ATII cells downregulate genes associated with surfactant and alveolar fluid clearance when exposed to inflammatory cytokines, and mesenchymal stromal cells partially reverse many of these gene expression changes.Mesenchymal stromal cells (MSCs) have therapeutic potential for the acute respiratory distress syndrome, and their benefits occur in part through interactions with alveolar type II (ATII) cells. We performed genome‐wide differential gene expression analyses of human ATII cells that were (1) unstimulated, (2) exposed to proinflammatory cytokines (CytoMix), or (3) exposed to CytoMix plus MSCs. Stimulation with CytoMix increased expression of inflammatory genes and downregulated genes related to surfactant function and alveolar fluid clearance, and several gene expression changes were reversed by exposure to MSCs.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/145579/1/phy213831_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145579/2/phy213831.pd