Effects of community health volunteers on infectious diseases of children under five in Volta Region, Ghana: study protocol for a cluster randomized controlled trial.

BACKGROUND: In many low- and middle-income countries, community health volunteers (CHVs) are employed as a key element of the public health system in rural areas with poor accessibility. However, few studies have assessed the effectiveness of CHVs in improving child health in sub-Saharan Africa through randomized controlled trials. The present study aims to measure the impact of health promotion and case management implemented by CHVs on the health of under-5 children in Ghana. METHODS/DESIGN: This study presents the protocol of a cluster-randomized controlled trial assessing the impacts of CHVs, in which the community was used as the randomization unit. A phase-in design will be adopted, and the intervention arm will be implemented in the intervention arm during the first phase and in the control arm during the second phase. The key intervention is the deployment of CHVs, who provide health education, provide oral rehydration solutions and zinc tablets to children with diarrhea, and diagnose malaria using a thermometer and a rapid diagnostic test kit during home visits. The primary endpoints of the study are the prevalence of diarrhea and fever/malaria in children under 5 years of age, as well as the proportion of affected children receiving case management for diarrhea and malaria. The first and second rounds of household surveys to collect data will be conducted in the first phase, and the final round will be conducted during the second phase. DISCUSSION: With growing attention paid to the roles of CHVs as an essential part of the community health system in low-income countries, this study will contribute valuable information to the body of knowledge on the effects of CHVs. TRIAL REGISTRATION: ISRCTN49236178 . (June 16th, 2015)

Quantitative analysis of mitochondrial DNA in porcine-mouse cloned embryos

The aim of the research is to identify that porcine oocytes can function as recipients for interspecies cloning and have the ability to develop to blastocysts. Furthermore each mitochondrial DNA (mtDNA) in interspecises cloned embryos was analyzed. For the study, mouse-porcine and porcine-porcine cloned embryos were produced with mouse fetal fibroblasts (MFF) and porcine fetal fibroblasts (PFF), respectively, introduced as donor cells into enucleated porcine oocytes. The developmental rate and cell numbers of blastocysts between intraspecies porcine-porcine and interspecies mouse-porcine cloned embryos were compared and real-time polymerase chain reaction (PCR) was performed for the estimate of mouse and porcine mtDNA copy number in mouse-porcine cloned embryos at different stages.There was no significant difference in the developmental rate or total blastocyst number between mouse-porcine cloned embryos and porcine-porcine cloned embryos (11.1 ± 0.9%, 25 ± 3.5 vs. 10.1 ± 1.2%, 24 ± 6.3). In mouse-porcine reconstructed embryos, the copy numbers of mouse somatic cell-derived mtDNA decreased between the 1-cell and blastocyst stages, whereas the copy number of porcine oocyte-derived mtDNA significantly increased during this period, as assessed by real-time PCR analysis. In our real-time PCR analysis, we improved the standard curve construction-based method to analyze the level of mtDNA between mouse donor cells and porcine oocytes using the copy number of mouse beta-actin DNA as a standard. Our findings suggest that mouse-porcine cloned embryos have the ability to develop to blastocysts in vitro and exhibit mitochondrial heteroplasmy from the 1-cell to blastocyst stages and the mouse-derived mitochondria can be gradually replaced with those of the porcine oocyte in the early developmental stages of mouse-porcine cloned embryos

Effects of improved sanitation on diarrheal reduction for children under five in Idiofa, DR Congo: a cluster randomized trial.

BACKGROUND: The lack of safe water and sanitation contributes to the rampancy of diarrhea in many developing countries. METHODS: This study describes the design of a cluster-randomized trial in Idiofa, the Democratic Republic of the Congo, seeking evidence of the impact of improved sanitation on diarrhea for children under four. Of the 276 quartiers, 18 quartiers were randomly allocated to the intervention or control arm. Seven hundred and-twenty households were sampled and the youngest under-four child in each household was registered for this study. The primary endpoint of the study is diarrheal incidence, prevalence and duration in children under five. DISCUSSION: Material subsidies will be provided only to the households who complete pit digging plus superstructure and roof construction, regardless of their income level. This study employs a Sanitation Calendar so that the mother of each household can record the diarrheal episodes of her under-four child on a daily basis. The diary enables examination of the effect of the sanitation intervention on diarrhea duration and also resolves the limitation of the small number of clusters in the trial. In addition, the project will be monitored through the 'Sanitation Map', on which all households in the study area, including both the control and intervention arms, are registered. To avoid information bias or courtesy bias, photos will be taken of the latrine during the household visit, and a supervisor will determine well-equipped latrine uptake based on the photos. This reduces the possibility of recall bias and under- or over-estimation of diarrhea, which was the main limitation of previous studies. TRIAL REGISTRATION: The study was approved by the Institutional Review Board of the School of Public Health, Kinshasa University (ESP/CE/040/15; April 13, 2015) and registered as an International Standard Randomized Controlled Trial (ISRCTN: 10,419,317) on March 13, 2015

The first reported hepatitis E outbreak in a food manufacturing factory: Korea, 2022

Objectives On February 16, 2022, 12 cases of hepatitis E virus (HEV) infection were reported in a food manufacturing factory in Korea. The aim of this study was to identify additional cases and to determine the source of this HEV outbreak. Methods This study was an in-depth investigation of 12 HEV immunoglobulin M (IgM)-positive cases and their demographic, clinical, and epidemiological characteristics. On-site specimens were collected from the environment and from humans, and a follow-up investigation was conducted 2 to 3 months after the outbreak. Results Among 80 production workers in the factory, 12 (15.0%) had acute HEV infection, all of whom were asymptomatic. The follow-up investigation showed that 3 cases were HEV IgM-positive, while 6 were HEV IgG-positive. HEV genes were not detected in the HEV IgM-positive specimens. HEV genes were not detected in the food products or environmental specimens collected on-site. HEV was presumed to be the causative pathogen. However, it could not be confirmed that the source of infection was common consumption inside the factory. Conclusion This was the first domestic case of an HEV infection outbreak in a food manufacturing factory in Korea. Our results provide information for the future control of outbreaks and for the preparation of measures to prevent domestic outbreaks of HEV infection

Machine Learning Classification of Buildings for Map Generalization

A critical problem in mapping data is the frequent updating of large data sets. To solve this problem, the updating of small-scale data based on large-scale data is very effective. Various map generalization techniques, such as simplification, displacement, typification, elimination, and aggregation, must therefore be applied. In this study, we focused on the elimination and aggregation of the building layer, for which each building in a large scale was classified as “0-eliminated,” “1-retained,” or “2-aggregated.” Machine-learning classification algorithms were then used for classifying the buildings. The data of 1:1000 scale and 1:25,000 scale digital maps obtained from the National Geographic Information Institute were used. We applied to these data various machine-learning classification algorithms, including naive Bayes (NB), decision tree (DT), k-nearest neighbor (k-NN), and support vector machine (SVM). The overall accuracies of each algorithm were satisfactory: DT, 88.96%; k-NN, 88.27%; SVM, 87.57%; and NB, 79.50%. Although elimination is a direct part of the proposed process, generalization operations, such as simplification and aggregation of polygons, must still be performed for buildings classified as retained and aggregated. Thus, these algorithms can be used for building classification and can serve as preparatory steps for building generalization

The porous hematite photoanodes for enhanced photoelectrochemical water splitting

Department of Energy Engineering (Energy Engineering)Due to the declining fossil fuel and growing energy demands, alternative and sustainable energy have been required. Developing clean, sustainable and renewable energy, such as hydrogen energy, is an important challenge to meet the energy demands. Photoelectrochemical (PEC) water splitting has been attracted as a promising strategy to produce hydrogen energy for resolving energy demands. Hematite (Fe2O??3) is one of the most promising PEC materials for solar water splitting. High charge recombination, poor conductivity is the problems of photoelectrochemical water splitting using hematite (Fe2O3) photoanode. Herein, we fabricate a porous P, Ti-hematite (Ti-Fe2O3) photoanode via a facile hydrothermal method for PEC water splitting. P, Ti-Fe2O3 photoanodes improve the conductivity and enhance the electron and hole transfer mobility. The significantly increased water splitting photocurrent density is attributed to the effect of P-doped (1.7-fold higher than pristine Ti-Fe2O3). Furthermore, P-doped Ti- Fe2O3 shows high surface area (12.3 m2 g-1) and large pore volume (2.8259 cm3 g-1) than pristine Ti- Fe2O3. Furthermore, this porous structure of hematite improves the short hole diffusion length. Therefore, the fabricated hematite photoanodes may provide an efficient photoanode for practical PEC water splitting.clos

The Role of Accent on East Asians’ Leadership

Not only do leaders influence followers, but followers also play a vital role in shaping leadership. Through two studies, I examined whether East Asian leaders with a foreign accent would be less respected by their followers than those without an accent. Additionally, I studied whether receiving respect from followers would affect foreign-accented East Asians’ leadership. In an experimental study (N = 150), I found that a foreign-accented East Asian leader was perceived as less effective and relatively poor at facilitating followers to cooperate toward a group goal than an East Asian leader without an accent. As a result, a foreign-accented East Asian leader was perceived as having less legitimate power to influence others. In Study 2 (N = 181), I surveyed actual leaders and found that foreign-accented East Asian leaders perceived less respect from followers than non-accented East Asian leaders and White leaders with and without a foreign accent. Moreover, foreign-accented East Asian leaders reported significantly more negative leader outcomes (leader identity strength and leadership self-efficacy) than foreign-accented and non-accented White leaders. These findings suggest that difficulties foreign-accented East Asian leaders face in the workplace may not be illuminated if the focus is only on race