104 research outputs found
Investigation of Impurity Tracer Transport in High Density Plasmas at the Stellarator Wendelstein 7-AS
Phosphorylation of Ubc9 by Cdk1 Enhances SUMOylation Activity
Increasing evidence has pointed to an important role of SUMOylation in cell cycle regulation, especially for M phase. In the current studies, we have obtained evidence through in vitro studies that the master M phase regulator CDK1/cyclin B kinase phosphorylates the SUMOylation machinery component Ubc9, leading to its enhanced SUMOylation activity. First, we show that CDK1/cyclin B, but not many other cell cycle kinases such as CDK2/cyclin E, ERK1, ERK2, PKA and JNK2/SAPK1, specifically enhances SUMOylation activity. Second, CDK1/cyclin B phosphorylates the SUMOylation machinery component Ubc9, but not SAE1/SAE2 or SUMO1. Third, CDK1/cyclin B-phosphorylated Ubc9 exhibits increased SUMOylation activity and elevated accumulation of the Ubc9-SUMO1 thioester conjugate. Fourth, CDK1/cyclin B enhances SUMOylation activity through phosphorylation of Ubc9 at serine 71. These studies demonstrate for the first time that the cell cycle-specific kinase CDK1/cyclin B phosphorylates a SUMOylation machinery component to increase its overall SUMOylation activity, suggesting that SUMOylation is part of the cell cycle program orchestrated by CDK1 through Ubc9
Untersuchungen zum Verunreinigungstransport von Aluminium und Eisen im Stellarator W7-AS mittels Laser-Ablationstechnik
Untersuchungen zum Verunreinigungstransport von Aluminium und Eisen im Stellarator W7-AS mittels Laser-Ablationstechnik
Discovery of Polyhydroxyalkyl Pyrazine Generation upon Coffee Roasting by In-Bean Labeling Experiments
Bestimmung von proteingebundener 2-Amino-6-(2-formyl-5-hydroxymethyl-1-pyrrolyl)-hexans�ure (?Pyrralin?) mittels Ionenaustauschchromatographie und Photodiodenarray-Detektion
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