First report on a pupping area of the tope shark Galeorhinus galeus (Carcharhiniformes, Triakidae) in the south-west Atlantic

Neonates of the tope shark Galeorhinus galeus are captured in sport and recreational coastal fisheries from January to April each year in Engaño Bay (42° 58′–43° 41′ S), Chubut, Argentina. The presence of these neonates is the first evidence of a pupping area for this shark in the south-west Atlantic Ocean. Knowledge of the pupping areas of chondrichthyans is important for establishing conservation measures and appropriate fishing regulations.Fil: Bovcon, Nelson Darío. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew. Departamento de Biología. Laboratorio de Hidrobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cochia, Pablo D.. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew. Departamento de Biología. Laboratorio de Hidrobiología; ArgentinaFil: Navoa, Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew. Departamento de Biología. Laboratorio de Hidrobiología; ArgentinaFil: Ledesma, Pablo Jeronimo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew. Departamento de Biología. Laboratorio de Hidrobiología; ArgentinaFil: Caille, Guillermo Martín. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew. Departamento de Biología. Laboratorio de Hidrobiología; ArgentinaFil: Baigún, Claudio Rafael M.. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentin

Dose-dependent significance of monosaccharides on intracellular α-L-rhamnosidase activity from Pseudoalteromonas sp.

Intracellular α-L-rhamnosidase (EC 3.2.1.40) from the psychrotolerant Pseudoalteromonas sp. 005NJ showed a dose-dependent inhibition for L-rhamnose (IC50 = 20 mM) and d-ribose (IC50 = 95 mM), whereas d-glucose and l-fucose presented a lower inhibition, with IC50 values as high as >0.5 and >0.2 M, respectively. On the other hand, d-fructose enhanced enzyme activity threefold, reaching a plateau of maximum specific activity between 0.2 and 0.4 M of this monosaccharide. Both effects, low inhibition and stimulation, caused by key fruit sugars (glucose and fructose), make this biocatalyst an interesting system in terms of its potential application for debittering fruit juices.Fil: Mazzaferro, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Orrillo, Alfredo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ledesma, Pablo Jeronimo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentin

Biosilagem de desperdício de merluza e farinha de cevada fermentados com bactérias ácido lácticas selecionadas

Suitability of Lactococcus lactis Tw34 and Lactobacillus plantarum Lb7 asbio-silage inoculants was studied. Experiments were carried out with a mixtureof hake (Merluccius hubbsi) by-products and barley meal fermentedduring 7 days. A sample acidified with lactic acid was used as control. Inbiological mixtures, the pH dropped below 5,0 after 2 days of fermentationand remained stable until the end of the experience. Maximum populations(> 109 CFU/g) were reached after 5 days of incubation at 18°C. Water solublepeptides concentration increased during the 7 days of incubation andno significant differences (p 0,05). After 7 days, phosphorousconcentrations reached 2.26 and 2,42 g/100 g in bio-silages fermented withLc. lactis Tw34 and Lb. plantarum Lb7, respectively, while control values remainsalmost stable (1,61 g/100 g). At the end of the experience, inhibitoryactivity of trypsin was abolished in both bio-silage while in control sampleantinutritional factors remained active. The results indicated the feasibility ofthe use of the selected mixture as substrate for bio-silage production andthe effectiveness Lc. lactis Tw34 and Lb. plantarum Lb7 as inoculants.Suitability of Lactococcus lactis Tw34 and Lactobacillus plantarum Lb7 as bio-silage inoculants was studied. Experiments were carried out with a mixture of hake (Merluccius hubbsi) by-products and barley meal fermented during 7 days. A sample acidified with lactic acid was used as control. In biological mixtures, the pH dropped below 5,0 after 2 days of fermentation and remained stable until the end of the experience. Maximum populations (> 109 CFU/g) were reached after 5 days of incubation at 18°C. Water soluble peptides concentration increased during the 7 days of incubation and no significant differences (p 0,05). After 7 days, phosphorous concentrations reached 2.26 and 2,42 g/100 g in bio-silages fermented with Lc. lactis Tw34 and Lb. plantarum Lb7, respectively, while control values remains almost stable (1,61 g/100 g). At the end of the experience, inhibitory activity of trypsin was abolished in both bio-silage while in control sample antinutritional factors remained active. The results indicated the feasibility of the use of the selected mixture as substrate for bio-silage production and the effectiveness Lc. lactis Tw34 and Lb. plantarum Lb7 as inoculants.Foi estudada a capacidade de inoculantes em biosilagem de Lactococcus lactis Tw34 y Lactobacillus plantarum Lb7. Os experimentos foram realizados com uma mistura de produtos de merluza (Merluccius hubbsi) e farinha de cevada, fermentada durante 7 dias. Como controle se utilizou uma amostra acidificada com ácido láctico. Nas misturas biológicas, o pH ficou abaixo 5,0 depois de 2 dias de fermentação e permaneceu estável até o final do experimento. A contagem máxima de viáveis (>109 UFC/g) foi alcançada depois de 5 dias de incubação a 18°C. A concentração de peptídeos solúveis em água aumentou durante os 7 dias de incubação e não houve diferença significativa (p 0,05). Depois de 7 dias, as concentrações de fósforo alcançadas foram de 2,26 e 2,42 g/100 g nas silagens fermentadas com Lc. lactis Tw34 e Lb. plantarum Lb7 respectivamente, en quanto que no controle os valores permaneceram quase estáveis (1,61 g/100 g). No final do experimento, a atividade inibitória de tripsina foi suprimida em ambas silagens enquanto que no controle os fatores antinutricionais continuavam ativos. Os resultados indicam a possibilidade do uso de uma mistura selecionada como substrato para a produção de silagem e a capacidade de Lc. lactis Tw34 e Lb. plantarum Lb7 como inoculantes.Fil: Marguet, Emilio Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; ArgentinaFil: Vallejo, Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; ArgentinaFil: Schulman, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; ArgentinaFil: Ibañez, Cecilia Mariel. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; ArgentinaFil: Ledesma, Pablo Jeronimo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; ArgentinaFil: Parada, Romina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentin