Tumor necrosis factor-alpha regulates the expression of inducible costimulator receptor ligand on CD34+ progenitor cells during differentiation into antigen presenting cells

The inducible costimulator receptor (ICOS) is a third member of the CD28 receptor family that regulates T cell activation and function. ICOS binds to a newly identified ligand on antigen presenting cells different from the CD152 ligands CD80 and CD86. We used soluble ICOSIg and a newly developed murine anti-human ICOS ligand (ICOSL) monoclonal antibody to further characterize the ICOSL during ontogeny of antigen presenting cells. In a previous study, we found that ICOSL is expressed on monocytes, dendritic cells, and B cells. To define when ICOSL is first expressed on myeloid antigen presenting cells, we examined ICOSL expression on CD34 cells in bone marrow. We found that CD34bright cells regardless of their myeloid commitment were ICOSL , whereas ICOSL was first expressed when CD34 expression diminished and the myeloid marker CD33 appeared

Gyroscopes based on nitrogen-vacancy centers in diamond

We propose solid-state gyroscopes based on ensembles of negatively charged nitrogen-vacancy (${\rm NV^-}$) centers in diamond. In one scheme, rotation of the nitrogen-vacancy symmetry axis will induce Berry phase shifts in the ${\rm NV^{-}}$ electronic ground-state coherences proportional to the solid angle subtended by the symmetry axis. We estimate sensitivity in the range of $5\times10^{-3} {\rm rad/s/\sqrt{Hz}}$ in a 1 ${\rm mm^3}$ sensor volume using a simple Ramsey sequence. Incorporating dynamical decoupling to suppress dipolar relaxation may yield sensitivity at the level of $10^{-5} {\rm rad/s/\sqrt{Hz}}$. With a modified Ramsey scheme, Berry phase shifts in the ${\rm ^{14}N}$ hyperfine sublevels would be employed. The projected sensitivity is in the range of $10^{-5} {\rm rad/s/\sqrt{Hz}}$, however the smaller gyromagnetic ratio reduces sensitivity to magnetic-field noise by several orders of magnitude. Reaching $10^{-5} {\rm rad/s/\sqrt{Hz}}$ would represent an order of magnitude improvement over other compact, solid-state gyroscope technologies.Comment: 3 figures, 5 page

Influence of Mechanical Layering and Natural Fractures on Undercutting and Rapid Headward Erosion (Recession) at Canyon Lake Spillway, Texas, U.S.A

This study investigates the role of mechanical layering and fractures on flood-related erosional undercutting and resulting rapid spillway recession. In the summer of 2002, 86 cm of rain fell in an 8-day period across the Guadalupe River drainage basin in central Texas, causing Canyon Lake reservoir to completely fill and overtop the emergency spillway for the first time. The resulting flood incised a gorge into the mechanically layered Glen Rose Formation and caused headward erosion (recession) at the downstream edge of the emergency spillway. Comparison of pre- and post-flood imagery and assessment of flood records indicates that maximum recession localized at the northern end of the emergency spillway where 28 m recession occurred. This recession occurred at an estimated rate of up to 10 m/day during the first ~3 days of the flood, which is among the highest rates of recorded bedrock recession. Analysis of historical photographs, field observations and measurement of erosional undercutting, along with measurements of fracture orientation, fracture spacing, and mechanical rebound are used to understand rock mass characteristics that influenced erosional undercutting and rapid recession of the spillway. Evidence of significant undercutting was observed where incompetent argillaceous wackestone (marl) underlies competent limestone. These results reveal that the greatest amount and rate of recession of the spillway was associated with undercutting and toppling collapse of fracture-bounded limestone blocks. Block size may be a factor in continuation of the process, in that large blocks may accumulate at the base of the scarp and inhibit continued erosional undercutting, whereas in other areas smaller eroded blocks can be carried away by the floodwaters and undercutting may continue, facilitating recession. The combination of mechanical contrast between layers and natural fractures in competent layers together contributed to exceptionally high rates of headward erosion. Observed rock mass erodibility behavior was in the range of medium to high erodibility in limestone with widely spaced fractures that would normally be expected to have very low erodibility. Bulk rock mass erodibility in this situation was similar to the most erodibile layer, specifically, the marl at base of spillway pour-off cliff

Temperature dependence of the nitrogen-vacancy magnetic resonance in diamond

The temperature dependence of the magnetic resonance spectra of nitrogen-vacancy (NV-) ensembles in the range of 280-330 K was studied. Four samples prepared under different conditions were studied with NV- concentrations ranging from 10 ppb to 15 ppm. For all of these samples, the axial zero-field splitting (ZFS) parameter, D, was found to vary significantly with temperature, T, as dD/dT = -74.2(7) kHz/K. The transverse ZFS parameter, E, was non-zero (between 4 and 11 MHz) in all samples, and exhibited a temperature dependence of dE/(EdT) = -1.4(3) x 10^(-4) K^(-1). The results might be accounted for by considering local thermal expansion. The observation of the temperature dependence of the ZFS parameters presents a significant challenge for room-temperature diamond magnetometers and may ultimately limit their bandwidth and sensitivity.Comment: 5 pages, 2 figures, 1 tabl

Hybridization of seedless grapes

Complete seedlessness was not achieved in any seedless x seedless families produced. This clearly shows the seedless trait is not controlled by a single recessive gene.The percentage of seedlessness obtained was not the same for all families. This indicates the importance of parental combinations and the need for progeny testing to increase the efficiency of producing seedless offspring.All seedless genotypes used except Thompson Seedless have a seeded female parent, indicating heterogenity for seeds/aborted seeds. It will be interesting now to use seedlings from the seedless x seedless families to see if 100 % seedless offspring can be obtained.Three seedless x seedless families compared to their complementary families from seeded x seedless were significantly different for percent seedless. In ovulo embryo rescue of seedless x seedless hybrids is also advantageous as it allows direct hybridization without seeded genotypes. This eliminates the introduction of genes from seeded genotypes and one generation - a savings of 5 years. The ability to achieve complimentary crosses between seedless genotypes directly can be achieved

Spin-exchange relaxation free magnetometry with Cs vapor

We describe a Cs atomic magnetometer operating in the spin-exchange relaxation-free (SERF) regime. With a vapor cell temperature of $103^\circ\rm{C}$ we achieve intrinsic magnetic resonance widths $\Delta B=17 {\rm \mu G}$ corresponding to an electron spin-relaxation rate of $300 {\rm s^{-1}}$ when the spin-exchange rate is $\Gamma_{SE}=14000 {\rm s^{-1}}$. We also observe an interesting narrowing effect due to diffusion. Signal-to-noise measurements yield a sensitivity of about $400\thinspace{\rm pG/\sqrt{Hz}}$. Based on photon shot noise, we project a sensitivity of $40 {\rm pG/\sqrt{Hz}}$. A theoretical optimization of the magnetometer indicates sensitivities on the order of $2 {\rm pG/\sqrt{Hz}}$ should be achievable in a $1 {\rm cm^3}$ volume. Because Cs has a higher saturated vapor pressure than other alkali metals, SERF magnetometers using Cs atoms are particularly attractive in applications requiring lower temperatures.Comment: 8 pages, 6 figures. submitted to PR

Physical properties of thermoelectric zinc antimonide using first-principles calculations

We report first principles calculations of the structural, electronic, elastic and vibrational properties of the semiconducting orthorhombic ZnSb compound. We study also the intrinsic point defects in order to eventually improve the thermoelectric properties of this already very promising thermoelectric material. Concerning the electronic properties, in addition to the band structure, we show that the Zn (Sb) crystallographically equivalent atoms are not exactly equivalent from the electronic point of view. Lattice dynamics, elastic and thermodynamic properties are found to be in good agreement with experiments and they confirm the non equivalency of the zinc and antimony atoms from the vibrational point of view. The calculated elastic properties show a relatively weak anisotropy and the hardest direction is the y direction. We observe the presence of low energy modes involving both Zn and Sb atoms at about 5-6 meV, similarly to what has been found in Zn4Sb3 and we suggest that the interactions of these modes with acoustic phonons could explain the relatively low thermal conductivity of ZnSb. Zinc vacancies are the most stable defects and this explains the intrinsic p-type conductivity of ZnSb.Comment: 33 pages, 8 figure