8 research outputs found
Secretome analysis of breast cancer-associated adipose tissue to identify paracrine regulators of breast cancer growth
Adipose tissue secretes a plethora of adipokines as evidenced by characterization of subcutaneous and visceral adipose tissue secretomes. However, adipose tissue composition and secretion pattern is depot and disease dependent, influencing the adipose tissue secretome. We investigated the secretome of cancer-associated adipose tissue (CAAT) explants from breast cancer patients and explored its role in breast cancer proliferation. CAAT proteins were identified by LC-MS/MS and human protein antibody arrays and stimulated proliferation of three breast cancer cell lines. Kinomics and transcriptomics of MCF-7 breast cancer cells treated with the secretome of CAAT revealed activation of Akt-, ERK- and JNK-pathways and differential expression of activator protein 1 (AP-1) and cAMP responsive element-binding protein (CREB) target genes. The cyclin-dependent kinase (CDK) 4/6-inhibitor palbociclib significantly abrogated CAAT-enhanced breast cancer cell proliferation. Our work characterizes the specific breast CAAT protein secretome and reveals its pro-proliferative potency in breast cancer
Atypical spindle cell/pleomorphic lipomatous tumor
Atypical spindle cell/pleomorphic
lipomatous tumor (ASPLT) is a recently described
morphologically low-grade and clinically indolent
adipocytic tumor, which will be incorporated as a new
tumor entity in the upcoming 5th edition of the WHO
Classification of Soft tissue and Bone tumors.
Histologically, ASPLTs are characterized by ill-defined
tumor margins and the presence of variable proportions
of mild-to-moderately atypical spindle cells, adipocytes,
lipoblasts, pleomorphic multinucleated cells and a
myxoid or collagenous extracellular matrix. ASPLTs can
show a wide variety of microscopic appearances and
there is histologic overlap with diverse mimics. The
diagnosis of ASPLT can therefore be challenging.
Molecular studies have shown a consistent absence of
MDM2 or CDK4 amplification. On the other hand,
deletions or losses of 13q14, including RB1, have been
identified in a significant subset of cases. This review
provides an overview of the currently known clinical and
pathological features of ASPLTs, detailing its most
relevant differential diagnoses
Dedifferentiated liposarcoma of the retroperitoneum with heterologous osteosarcomatous differentiation and a striking aneurysmal bone cyst-like morphology
A 69-year-old woman with a 10-year medical history of recurrent retroperitoneal dedifferentiated liposarcoma presented with a 3-cm large hemorrhagic and multicystic left-sided retroperitoneal mass. Histopathological examination of the resected specimen showed a heterogeneous, high-grade mesenchymal nonlipogenic tumor with areas of osteoblastic/osteosarcomatous differentiation and aneurysmal bone cyst-like features. Based on the clinical presentation, the morphology, and the supportive immunohistochemical and molecular findings (MDM2 overexpression and amplification of the MDM2 gene, respectively), a diagnosis of a dedifferentiated liposarcoma with heterologous osteosarcomatous differentiation and an aneurysmal bone cyst-like morphology was made. To the best of our knowledge, this is the first description of aneurysmal bone cyst-like morphology in dedifferentiated liposarcoma, further expanding the broad morphological spectrum of dedifferentiated liposarcoma