Infecção por múltiplos tipos de Papilomavirus humano em mulheres jovens sexualmente ativas

Modelo do estudo: transversal. Objetivo do estudo: estimar a frequência e a distribuição dos tipos de Papilomavírus humano (HPV) em mulheres jovens sexualmente ativas. Metodologia: foram coletadas amostras do canal vaginal e endocérvice de 158 mulheres, com idade entre 18 e 35 anos, por meio da técnica de autocoleta. O DNA-HPV foi extraído e amplificado pela técnica de PCR end point utilizando os primers consensus PGMY09/11. A genotipagem foi executada pela técnica de PCR tipo específico (TSPCR) e por análise dos fragmentos obtidos com o uso de enzimas de restrição (RFLP). Resultados: DNA-HPV foi detectado em 23% das amostras, 92% foram genótipos de alto risco oncogênico (HR), sendo prevalente o HPV45, seguido do HPV16 e 31. Infecções por múltiplos tipos de HPV foram detectadas em 35% das amostras, demonstrando infecção por mais de dois tipos em 22,2% das genotipadas. Maior frequência do DNA-HPV foi observada entre mulheres com idade ≤ 25 anos. Conclusão: Este estudo demostrou alta frequência da infecção pelo HPV em mulheres jovens sexualmente ativas, assim como uma alta prevalência de infecção múltipla com tipos de alto risco oncogênicoStudy design: cross. Study objective: estimate the frequency and distribution types of human papillomavirus (HPV) in sexually active young women. Methods: samples of vaginal and endocervical canal of 158 women, aged between 18 and 35 years were collected through self-collection technique. The HPV DNA was extracted and amplified by PCR end point using the consensus primers PGMY09/11 and genotyping was performed by PCR specific type (TS-PCR) and by analysis of the fragments obtained from the use of restriction enzymes (RFLP). Results: HPV DNA was detected in 23% of participants, 92% were high-risk HPV genotypes (HR), being the prevalent HPV45, followed by HPV16 and 31 infections with multiple HPV types were detected in 35% of samples, indicating infection by more than two types in 22.2% of the genotyped. Higher frequency of HPV DNA was detected in women ≤ 25 old. Conclusion: this demonstrated a high frequency of HPV infection in sexually active young women, as well as a high prevalence of infection with multiple types of high oncogenic ris

Presence of highly oncogenic human papillomavirus in the oral mucosa of asymptomatic men

Objectives: The aim of this study was to identify highly oncogenic forms of human papillomavirus in the oral mucosa of asymptomatic men. Methods: In this study, we analyzed samples of exfoliated cells from the oral cavity of 559 asymptomatic men. DNA-human papillomavirus was detected using the consensus primers PGMY09/11; viral genotyping was performed using type-specific PCR and restriction fragment length polymorphism. Results: DNA-human papillomavirus was detected in 1.3% of the study participants and of those 42.8% were infected by more than one type of virus. Viral types included HPV6, 11, 89 (low oncogenic risk), and HPV52, 53 (high oncogenic risk). Increased vulnerability to human papillomavirus infection was observed in individuals aged over 26 years, among those who reported oral sex practices, and in those who have had more than 16 sexual partners since first engaging in sexual intercourse. Conclusions: There was a low prevalence of human papillomavirus detection in the oral mucosa of asymptomatic men. Highly oncogenic human papillomavirus types and infection by more than one viral type was observed. Oral sex practices and a large number of sexual partners may increase the risk of acquiring human papillomavirus infection. Keywords: Human papilomavírus, Men, Viral type, Oral mucosa