Design, Fabrication, and Application of Mini-Scaffolds for Cell Component in Tissue Engineering

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Cultura autóloga de células-tronco mesenquimais de tecido adiposo para o tratamento de rítides faciais

OBJETIVO: Testar o efeito das c élulas tronco mesenquimais (CTM) de tecido adiposo no preenchimento cutâneo de rítides na região naso-labial. MÉTODOS: Foram coletados 50 cc de gordura da região infra-umbilical e 20 mL de sangue periférico de 15 voluntárias do sexo feminino para obtenção das CTM e de plasma autólogo, respectivamente. As voluntárias foram agrupadas de acordo com as estratégias de injeções intra-dérmicas: grupo (1) somente o ácido hialurônico; grupo (2) somente as CTM; grupo (3) CTM associadas ao ácido hialurônico. Tratando-se de um estudo prospectivo e qualitativo o acompanhamento das voluntárias era mensal através de fotografias. RESULTADOS: No grupo (1) foi observado um efeito de preenchimento imediato ao contrário do grupo (2) onde o efeito de preenchimento pleno foi alcançado aproximadamente após dois meses. No grupo (3) o preenchimento ocorreu de maneira mais efetiva e também progressiva, devido à combinação dos efeitos de curto e de longo prazo gerados pelo ácido hialurônico e pelas CTM, respectivamente. CONCLUSÃO: As CTM quando associadas ao ácido hialurônico foram capazes de promover o preenchimento de sulcos profundos, com melhora progressiva do tônus da pele e diminuição das linhas de expressão

Design, Fabrication, and Application of Mini-Scaffolds for Cell Components in Tissue Engineering

The concept of “lockyballs” or interlockable mini-scaffolds fabricated by two-photon polymerization from biodegradable polymers for the encagement of tissue spheroids and their delivery into the desired location in the human body has been recently introduced. In order to improve control of delivery, positioning, and assembly of mini-scaffolds with tissue spheroids inside, they must be functionalized. This review describes the design, fabrication, and functionalization of mini-scaffolds as well as perspectives on their application in tissue engineering for precisely controlled cell and mini-tissue delivery and patterning. The development of functionalized mini-scaffolds advances the original concept of “lockyballs” and opens exciting new prospectives for mini-scaffolds’ applications in tissue engineering and regenerative medicine and their eventual clinical translation

Characterization of stromal vascular fraction and adipose stem cells from subcutaneous, preperitoneal and visceral morbidly obese human adipose tissue depots

Submitted by Sandra Infurna ([email protected]) on 2017-07-05T15:07:47Z No. of bitstreams: 1 sally_liechocki_etal_IOC_2017.pdf: 1981245 bytes, checksum: 3308a3561aa94a04244062a6765aaf38 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2017-07-05T15:37:48Z (GMT) No. of bitstreams: 1 sally_liechocki_etal_IOC_2017.pdf: 1981245 bytes, checksum: 3308a3561aa94a04244062a6765aaf38 (MD5)Made available in DSpace on 2017-07-05T15:37:48Z (GMT). No. of bitstreams: 1 sally_liechocki_etal_IOC_2017.pdf: 1981245 bytes, checksum: 3308a3561aa94a04244062a6765aaf38 (MD5) Previous issue date: 2017Universidade Federal do Rio de Janeiro. Programa de Pós-Graduação em Clínica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia. Laboratório de Bioengenharia de Tecidos. Duque de Caxias, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Laboratório de Bioengenharia de Tecidos. Duque de Caxias, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Nutrologia. Rio de Janeiro, RJ, Brasil..Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Cirurgia. Rio de Janeiro, RJ, Brasil..Universidade Federal do Rio de Janeiro. Programa de Pós-Graduação em Clínica Médica. Rio de Janeiro, RJ, Brasil / Faculdade de Medicina de Petrópolis. Centro de Medicina Regenerativa. Petrópolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Laboratório de Bioengenharia de Tecidos. Duque de Caxias, RJ, Brasil / Universidade Federal do Rio de Janeiro. Núcleo de Pesquisa Multidisciplinar em Xerém, Duque de Caixas, RJ, Brasil.The pathological condition of obesity is accompanied by a dysfunctional adipose tissue. We postulate that subcutaneous, preperitoneal and visceral obese abdominal white adipose tissue depots could have stromal vascular fractions (SVF) with distinct composition and adipose stem cells (ASC) that would differentially account for the pathogenesis of obesity

An alternative method for the isolation of mesenchymal stromal cells derived from lipoaspirate samples

11 p. : il.Background aims Since initial methods were developed for isolating cells from adipose tissue, little has been done to improve mesenchymal stromal cell (MSC) yield. The aim of the present study was to isolate a population of MSC from lipoaspirate samples without tissue digestion and to assess the possibility of cryopreserving the freshly isolated cells. Methods A population of MSC was isolated from 13 patients’ lipoaspirate samples by mechanical dissociation. Mechanically processed lipoaspirate adipose tissue (MPLA) cells were characterized after in vitro cell expansion by morphologic analysis, expression of MSC surface markers and differentiation assays. Results Mechanical dissociation yielded a large quantity of adherent MSC both after standard and vibro-assisted liposuction. Preservation of lipoaspirate samples at 4°C for 1 or 2 days until the mechanical procedure did not change the MPLA cell content. It was possible to store freshly isolated MPLA cells by cryopreservation without loss of the MSC population. Adherent MPLA cells were negative for CD45 and CD31 and positive for CD34, CD105, CD44 and CD90. They also showed adipogenic, osteogenic and chondrogenic potentials similar to MSC populations from other sources as already described in the literature. Conclusions MSC can be isolated from human lipoaspirate samples by the mechanical procedure described in this study with a signifi cant reduction in time and cost. Together with cryopreservation of freshly isolated MPLA cells, this has made it easier to harvest and store MSC for therapeutic applications such as soft-tissue augmentation and tissue engineering

Adult Stem Cells Spheroids to Optimize Cell Colonization in Scaffolds for Cartilage and Bone Tissue Engineering

Top-down tissue engineering aims to produce functional tissues using biomaterials as scaffolds, thus providing cues for cell proliferation and differentiation. Conversely, the bottom-up approach aims to precondition cells to form modular tissues units (building-blocks) represented by spheroids. In spheroid culture, adult stem cells are responsible for their extracellular matrix synthesis, re-creating structures at the tissue level. Spheroids from adult stem cells can be considered as organoids, since stem cells recapitulate differentiation pathways and also represent a promising approach for identifying new molecular targets (biomarkers) for diagnosis and therapy. Currently, spheroids can be used for scaffold-free (developmental engineering) or scaffold-based approaches. The scaffold promotes better spatial organization of individual spheroids and provides a defined geometry for their 3D assembly in larger and complex tissues. Furthermore, spheroids exhibit potent angiogenic and vasculogenic capacity and serve as efficient vascularization units in porous scaffolds for bone tissue engineering. An automated combinatorial approach that integrates spheroids into scaffolds is starting to be investigated for macro-scale tissue biofabrication

Differential lipid accumulation capacity and cytokine secretion of induced ASC derived from subcutaneous, preperitoneal and visceral depots.

<p>The ASC were cultured under lipid accumulation stimulus for up to 3 weeks. Lipid accumulation was analyzed by Oil Red O staining (A-C). The total Oil Red O dye captured was measured in a spectrophotometer. The Graph (D) shows the quantitative results of the Oil Red O staining. ANOVA tests comparing the absorbance were performed. Cytokine secretion in the supernatant of the induced cells is expressed in graphs (E) IL-6, (F) IL-8, (G) MCP-1, (H) adiponectin, (I) G-CSF, (J) PAI-1. <i>p</i> values under graphs resulted from statistical tests. The asterisks represent the <i>p</i> values from the statistical post-tests: (*) p <0.05. (A-C) Optical microscopy—bar size: 50 micrometers. Assays were performed in triplicates for each of the 3 patients evaluated. ASC: adipose stem cell; SC: subcutaneous; PP: preperitoneal; VC: visceral; IL: interleukin; MCP-1: Monocyte Chemoattractant Protein 1; G-CSF: Granulocyte colony-stimulating factor; PAI-1: Plasminogen activator inhibitor-1.</p