Dengue Virus 3 Genotype I in Aedes aegypti Mosquitoes and Eggs, Brazil, 2005–2006

Dengue virus type 3 genotype I was detected in Brazil during epidemics in 2002–2004. To confirm this finding, we identified this virus genotype in naturally infected field-caught Aedes aegypti mosquitoes and eggs. Results showed usefulness of virus investigations in vectors as a component of active epidemiologic surveillance

Dengue Virus 3 Genotype 1 Associated with Dengue Fever and Dengue Hemorrhagic Fever, Brazil

Dengue serotype 3 viruses were isolated from patients in Brazil from 2002 through 2004. On the basis of phylogenetic analyses, these isolates were assigned genotype 1. This genotype had never been reported in South America before. Its appearance indicates a major risk factor for dengue epidemics and severe disease

Molecular detection and phylogeny of bovine viral diarrhea virus 1 among cattle herds from Northeast, Southeast, and Midwest regions, Brazil

Submitted by Nuzia Santos ([email protected]) on 2019-08-27T15:42:26Z No. of bitstreams: 1 Molecular detection and phylogeny of bovine viral.pdf: 581803 bytes, checksum: 67af0bad980784eadc90b9fed2d954f5 (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2019-08-27T15:47:18Z (GMT) No. of bitstreams: 1 Molecular detection and phylogeny of bovine viral.pdf: 581803 bytes, checksum: 67af0bad980784eadc90b9fed2d954f5 (MD5)Made available in DSpace on 2019-08-27T15:47:18Z (GMT). No. of bitstreams: 1 Molecular detection and phylogeny of bovine viral.pdf: 581803 bytes, checksum: 67af0bad980784eadc90b9fed2d954f5 (MD5) Previous issue date: 2019Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil / Universidade Federal dos Vales do Jequitinhonha e Mucuri. Faculdade de Medicina. Diamantina, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia de Doenças Virais. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Pesquisa em Virologia Animal. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Virologia Básica e Aplicada. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil.We examined the circulating BVDV species and genotypes among cattle herds from Northeast, Southeast, and Midwest regions in Brazil. A total of 77 animals tested positive through standard PCR. Phylogenetic analyses revealed the presence of BVDV-1a, highlighting the need for better surveillance strategies to prevent BVDV spread in the country

Dengue Virus 2 American-Asian Genotype Identified during the 2006/2007 Outbreak in Piauí, Brazil Reveals a Caribbean Route of Introduction and Dissemination of Dengue Virus in Brazil

<div><p><i>Dengue virus</i> (DENV) is the most widespread arthropod-borne virus, and the number and severity of outbreaks has increased worldwide in recent decades. Dengue is caused by DENV-1, DENV- 2, DENV-3 and DENV-4 which are genetically distant. The species has been subdivided into genotypes based on phylogenetic studies. DENV-2, which was isolated from dengue fever patients during an outbreak in Piaui, Brazil in 2006/2007 was analyzed by sequencing the envelope (E) gene. The results indicated a high similarity among the isolated viruses, as well as to other DENV-2 from Brazil, Central America and South America. A phylogenetic and phylogeographic analysis based on DENV-2E gene sequences revealed that these viruses are grouped together with viruses of the American-Asian genotype in two distinct lineages. Our results demonstrate the co-circulation of two American-Asian genotype lineages in northeast Brazil. Moreover, we reveal that DENV-2 lineage 2 was detected in Piauí before it disseminated to other Brazilian states and South American countries, indicating the existence of a new dissemination route that has not been previously described.</p></div

Amino acid polymorphisms in the envelope protein of the American/Asian DENV-2 genotype.

<p>(Top) A diagram of the DENV-2 envelope protein showing its three protein domains (I, II and III). (Bottom) A partial alignment of the envelope protein showing sites of amino acid polymorphism within the American-Asian genotype. Other DENV-2 genotypes (Asian I, Asian II, Cosmopolitan and American) are shown for comparison. Amino acid sites in blue boxes are those that most likely had underwent a non-synonymous mutation after the divergence of the two lineages because they differ from the two ancient American-Asian DENV-2 samples (Jamaica/M20558/1983 and Puerto Rico/AY484607/1988). Amino acids are colored according to their side chain charge (hydrophobic: yellow; polar: green; negatively charged: red; and positively charged: blue).</p

Age and sex distribution of IgM-ELISA dengue positive patients from the 2006/2007 outbreak in Piauí, Brazil.

<p>NI – not informed.</p

Evolutionary relationship between DENV-2 isolates from Piauí and the five genotypes of DENV-2.

<p>A maximum clade credibility (MCC) tree was selected after Bayesian inference analysis (strict molecular clock; TN93+G; 400,000,000 iterations) of 72 DENV-2 envelope sequences. The five DENV-2 genotypes (American-Asian, American, Asian I, Asian II and Cosmopolitan) are highlighted in different colors. Brazilian isolates (bold blue letters) clustered within the American-Asian genotype and could be divided into two groups: lineage 1(dark green block) and lineage 2 (light green block). Each node is represented by colours black and red (), which presented posterior probability value>0.9 and >0.75, respectively. Blue bars represent the extent of the 95% highest probability density (95% HPD) for each divergence time. The most probable geographic state for each internal node was inferred by discrete phylogeographic analysis. Different colors in the branch represent distinct geographical states according to the legend on the left side of the figure. Branch width is proportional to the probability value of the inferred ancestral geographical state.</p

Description of DENV-2 isolates from Piauí, Brazil.

a<p>F is female and M is male.</p>b<p>Number of passages in cell culture (C6/36 cell line) are shown.</p

Bayesian coalescent and discrete phylogeographic analyses of Brazilian DENV-2 lineage 1 based on envelope nucleotide sequences.

<p>A maximum clade credibility tree was inferred by Bayesian inference analysis (strict molecular clock; TN93+G; 200,000,000 iterations) using 63 DENV-2 envelope sequences (summarized in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104516#pone.0104516.s003" target="_blank">Table S1</a>) retrieved by a BLAST search against the entire GenBank database and using PI-111/2006 (indicated by a red arrow) as a query.PI-111/2006 is an isolate from the state of Piauí (Brazil) that clustered in Brazilian DENV-2 lineage 1. Nodes that presented posterior probability value of >0.9 and >0.75 are represented by black and red circle (), respectively. Blue bars in each node represent the extent of the 95% highest probability density (95% HPD) for each divergence time. The most probable geographic state for each internal node was inferred by discrete phylogeographic analysis. Different colors in the branch represent distinct geographical states according to the legend on the left side of the figure. The branch width is proportional to the probability value of the inferred ancestral geographical state.</p

Geographic location of Piauí and its meso-regions.

<p>The state of Piauí is located in northeastern Brazil and is divided into the Mid-North, North Central, Southeast and Southwest regions. The state capital, Teresina, is indicated with an arrow on the map.</p