De novo copy number variations in candidate genomic regions in patients of severe autism spectrum disorder in Vietnam

Autism spectrum disorder (ASD) is a developmental disorder with a prevalence of around 1% children worldwide and characterized by patient behaviour (communication, social interaction, and personal development). Data on the efficacy of diagnostic tests using copy number variations (CNVs) in candidate genes in ASD is currently around 10% but it is overrepresented by patients of Caucasian background. We report here that the diagnostic success of de novo candidate CNVs in Vietnamese ASD patients is around 6%. We recruited one hundred trios (both parents and a child) where the child was clinically diagnosed with ASD while the parents were not affected. We performed genetic screening to exclude RETT syndrome and Fragile X syndrome and performed genome-wide DNA microarray (aCGH) on all probands and their parents to analyse for de novo CNVs. We detected 1708 non-redundant CNVs in 100 patients and 118 (7%) of them were de novo. Using the filter for known CNVs from the Simons Foundation Autism Research Initiative (SFARI) database, we identified six CNVs (one gain and five loss CNVs) in six patients (3 males and 3 females). Notably, 3 of our patients had a deletion involving the SHANK3 gene–which is the highest compared to previous reports. This is the first report of candidate CNVs in ASD patients from Vietnam and provides the framework for building a CNV based test as the first tier screening for clinical management

IMPACTS OF WATERING METHOD AND FREQUENCY ON SEVERAL BIOPHYSICS CHARACTERISTICS AND PRODUCTIVITY OF WAXY MAIZE (ZEA MAYS L.)

Cultivation technique is one of the important factors that helps improve plant productivity. Of which, using proper watering method can not only help plants grow faster but also help reduce water usage in the context of severe weather conditions that lead to water shortage for irrigation in agriculture. The research was conducted on rice land during Spring-Summer season in Chau Thanh A district, Hau Giang province and it aims at finding out suitable watering methods and frequency for maize (Zea mays L.) cultivation. The research includes 2 factors with 4 formal solutions with 4-time repetition. According to test results, plant height in A2B1 formal solution (method of bottom watering, once a day) is the highest one. Also, watering once a day makes the growing speed achieve the fastest one. Corncob weight and diameter, weight of 1000 kernels, productivity in theory and in reality of A1B2 (formal solution of sprinkler watering, once every 3 days) are the highest. Based on physiological maturity observation, A1B2 (formal solution of sprinkler watering, once every 3 days) and A2B2 (formal solution of bottom watering, once every 3 days) have the shortest physiological maturity. According to calculation of efficiency on the tested crops, investment cost is 1.044,22 USD while the profit is 857,13. It is also found out that using sprinkler watering once every 3 days can help maize crops in Spring-Summer season achieve higher productivity in Hau Giang province

Clinical features of three patients with paradoxical immune reconstitution inflammatory syndrome associated with Talaromyces marneffei infection

Talaromyces marneffei infection is a major cause of death in HIV-infected individuals in South and Southeast Asia. Talaromycosis immune reconstitution inflammatory syndrome has not been well described. Here we report the clinical features, management, and outcomes of three HIV-infected patients with talaromycosis-associated paradoxical immune reconstitution inflammatory syndrome in Ho Chi Minh City, Vietnam

Citrus diaspidids in Viet Nam : new, and confirmation of previous, records based on morphological and molecular verification of taxa

Armoured scales (Hemiptera: Diaspididae) belong to the largest scale insect family and are among the most invasive insects in the world. Accurate identification of armoured scales is essential for systematic and phylogenetic studies; biogeography; trade and plant quarantine; and pest management, particularly biological control. Several species are serious pests of citrus. Records of past field surveys conducted in Viet Nam between 1967 and 2010 indicated the presence of 28 species on citrus there. Discrepancies in these records, and the retention of specimens of only five species in collections, led us to undertake surveys throughout the citrus-growing regions of the country in 2013 and 2014 to verify previous records and conserve voucher specimens. The presence of 21 diaspidid species was confirmed based on morphological and molecular data. The species observed were common but rarely abundant. Populations in commercial orchards may have been influenced by use of pesticides, but most species were recorded also in gardens and orchards where pesticide use was uncommon. Natural enemies were abundant, but were not thoroughly documented for all the diaspidids we observed. An identification key to the species collected is provided. Differences between our findings and previous records from Viet Nam, from Yunnan and Guangxi in China, and from Indochinese countries neighbouring Viet Nam, indicate the need for extensive surveys to fully document the diaspidid fauna on citrus in the region

Dietary Effects of Carotenoid on Growth Performance and Pigmentation in Bighead Catfish (<i>Clarias macrocephalus</i> Günther, 1864)

This study investigates the effects of supplemental carotenoid pigments on growth and color performance in bighead catfish (Clarias macrocephalus). Two experiments were undertaken to determine the appropriate types, feed duration, and dose of astaxanthin (As), canthaxanthin (Ca), and xanthophyll (Xa) pigments individually and in combination. In the first experiment, fish were fed with one control diet (basic diet), six experimental diets comprised of three diets of As, Ca, and Xa at a 100 mg/kg rate of supplementation, respectively, and three diets combinations of As + Ca, As + Xa, and Ca + Xa at a supplement rate of 50 mg + 50 mg/kg. The results showed no significant difference in weight gain (WG), specific growth rate (SGR), survival rate (SR), and feed conversion ratio of fish among treatments (p > 0.05) after 6 weeks. The L* (Lightness) and a* (redness) values in the Xa diet were significantly lower than other treatments, while b* (yellowness) was significantly higher than in the control and others treatments (p 0.05). These values peaked after 4 weeks and remained stable until the end of the experiment. Consistently, the highest muscle carotenoid content (16.89 ± 0.60 mg/100 g) was found in the fish fed with the Xa diet. The Xa diet was selected for the second experiment. This experiment consisted of four Xa supplemented diets at rates of 25, 50, 75, and 100 mg/kg and a basal diet without any Xa supplementation. The results showed that there was no difference in the SGR or SR of fish fed various Xa levels (p > 0.05). Fish fed the Xa diet of 75 mg/kg were the most preferred by consumers for the natural “yellowness” of muscle. Thus, the results suggested that additional carotenoid pigments did not affect the growth performance of fish. Farmers and feed producers could utilize Xa at an optimal dose of 75 mg/kg to enhance color performance in the market size of bighead catfish for at least 4 weeks prior to harvest

Outcomes of autologous bone marrow mononuclear cell administration in the treatment of neurologic sequelae in children with spina bifida

Abstract Background To evaluate the safety and efficacy of autologous bone marrow mononuclear cell (BMMNC) infusion in the management of neurological sequelae in children with spina bifida (SB). Methods BMMNCs were harvested from bilateral anterior iliac crests. Two intrathecal BMMNC administrations were performed with an interval of 6 months. The measurements of outcomes included clinical assessments, cystomanometry and rectomanometry. Results Eleven children with SB underwent autologous BMMNC infusions from 2016 to 2020. There were no severe adverse events during the study period. The number of patients requiring assistance to expel stools decreased from 11 before cell infusion to 3 after the second cell infusion. The number of patients who had urine leakage decreased from 9 patients at baseline to 3 patients after the second BMMNC infusion. The mean bladder capacity increased from 127.7 ± 59.2 ml at baseline to 136.3 ± 54.8 ml at six months and to 158.3 ± 56.2 ml at 12 months after BMMNC infusions. Detrusor pressure (pdet) decreased from 32.4 ± 22.0 cm H2O at baseline to 21.9 ± 11.8 cm H2O after 12 months of follow-up. At baseline, six patients could walk independently. After the 2nd infusion, eight patients could walk independently. Conclusion Intrathecal infusions of autologous bone marrow mononuclear cells are safe and may improve bowel, bladder, and motor function in children with SB. Trial registration: NCT, NCT05472428. Registered July 25, 2022- Retrospectively registered, https://www.clinicaltrials.gov/ct2/show/NCT05472428

Improving the electrochemical performance of lithium‐ion battery using silica/carbon anode through prelithiation techniques

Abstract This work focuses on the two most common techniques, including the direct contact method (CM) and the electrochemical method (EM) in the half‐cell applied for the SiO2/C anode. After the prelithiation process, the anodes would be assembled in the coin cells paired with NMC622 cathode. According to electrochemical performance, prelithiation techniques could strengthen the initial discharged capacity and Coulombic efficiency. While the nonprelithiated sample exhibits a poor discharged capacity of 48.43 mAh·g−1 and low Coulombic efficiency of 87.41% in the first cycle, the CM and EM methods illustrated a better battery performance. Specifically, the EM4C exhibited a higher initial discharged capacity and Coulombic efficiency (137.06 mAh·g−1 and 95.82%, respectively) compared to the CM30 (99.08 mAh·g−1 and 93.23%, respectively). As a result, this research hopes to bring some remarkable information to improve full‐cell properties using SiO2/C as an anode material by the prelithiation method

De novo copy number variations in candidate genomic regions in patients of severe autism spectrum disorder in Vietnam.

Autism spectrum disorder (ASD) is a developmental disorder with a prevalence of around 1% children worldwide and characterized by patient behaviour (communication, social interaction, and personal development). Data on the efficacy of diagnostic tests using copy number variations (CNVs) in candidate genes in ASD is currently around 10% but it is overrepresented by patients of Caucasian background. We report here that the diagnostic success of de novo candidate CNVs in Vietnamese ASD patients is around 6%. We recruited one hundred trios (both parents and a child) where the child was clinically diagnosed with ASD while the parents were not affected. We performed genetic screening to exclude RETT syndrome and Fragile X syndrome and performed genome-wide DNA microarray (aCGH) on all probands and their parents to analyse for de novo CNVs. We detected 1708 non-redundant CNVs in 100 patients and 118 (7%) of them were de novo. Using the filter for known CNVs from the Simons Foundation Autism Research Initiative (SFARI) database, we identified six CNVs (one gain and five loss CNVs) in six patients (3 males and 3 females). Notably, 3 of our patients had a deletion involving the SHANK3 gene-which is the highest compared to previous reports. This is the first report of candidate CNVs in ASD patients from Vietnam and provides the framework for building a CNV based test as the first tier screening for clinical management