Mixed infections with distinct cytomegalovirus glycoprotein B genotypes in Polish pregnant women, fetuses, and newborns

The purpose of this investigation was to describe a distribution of cytomegalovirus (CMV) single and multiple genotypes among infected pregnant women, their fetuses, and newborns coming from Central Poland, as well as congenital cytomegaly outcome. The study involved 278 CMV-seropositive pregnant women, of whom 192 were tested for viral DNAemia. Human cytomegalovirus (HCMV) genotyping was performed for 18 of 34 pregnant women carrying the viral DNA and for 12 of their 15 offspring with confirmed HCMV infections. Anti-HCMV antibodies levels were assessed by chemiluminescence immunoassay (CLIA) and enzyme-linked fluorescence assay (ELFA) tests. Viral DNA loads and genotypes were determined by real-time polymerase chain reaction (PCR) assays for the UL55 gene. In the pregnant women, we identified HCMV gB1, gB2, gB3, and gB4 genotypes. Single gB2, gB3, or gB4 genotypes were observed in 14 (77.8 %) women, while multiple gB1–gB2 or gB2–gB3 genotypes were observed in four (22.2 %). Maternal HCMV genotypes determined the genotypes identified in their fetuses and newborns (p ≤ 0.050). Half of them were infected with single HCMV gB1, gB2, or gB3 genotypes and the other half with multiple gB1–gB2 or gB2–gB3 genotypes. Single and multiple genotypes were observed in both asymptomatic and symptomatic congenital cytomegaly, although no gB3 genotype was identified among asymptomatic cases. In Central Poland, infections with single and multiple HCMV strains occur in pregnant women, as well as in their fetuses and neonates, with both asymptomatic and symptomatic infections. HCMV infections identified in mothers seem to be associated with the viral genotypes in their children

Textile‐to‐rigid microstrip transition using permanent magnets

A novel transition between a rigid-substrate and a flexible-substrate microstrip for use in wearable devices is described. The contact force between the conductors of the two microstrips is supplied by a pair of rectangular magnets. A simulation and measurement study carried out on a test jig comprising a back-to-back pair of such transitions demonstrated an insertion loss of better than 0.5 dB/transition can be achieved in the range of 0-3 GHz

In vitro antileukemic activity of novel adenosine derivatives bearing boron cluster modification

A series of adenosine derivatives bearing a boron cluster were synthesized and evaluated for their cytotoxicity against primary peripheral mononuclear cells from the blood of 17 patients with leukemias (16 CLL and 1 very rare PLL), as well as from 5 healthy donors used as a control. Among the tested agents, two, i.e., compounds 1 and 2, displayed high in vitro cytotoxicity and proapoptotic potential on leukemic cells, with only scarce activity being seen against control cells. Biological tests related to apoptosis revealed the activation of the main execution apoptotic enzyme, procaspase-3, in CLL and PLL cells exposed to compounds 1 and 2. Moreover, the above compounds indicated high activity in the proteolysis of the apoptotic markers PARP-1 and lamin B1, fragmentation of DNA, and the induction of some changes in the expression of the Mcl-1, protein apoptosis regulator in comparison with control cells

Probing BRD inhibition substituent effects in bulky analogues of (+)-JQ1

A series of bulky organometallic and organic analogues of the bromodomain (BRD) inhibitor (+)-JQ1 have been prepared. The most potent, N-[(adamantan-1-yl)methyl]-2-[(9S)-7-(4-chlorophenyl)-4,5,13-trimethyl-3-thia-1,8,11,12-tetraazatricyclo[8.3.0.02,6]trideca-2(6),4,7,10,12-pentaen-9-yl]acetamide, 2e, showed excellent potency with an KD=ca. 130 nm vs. BRD4(1) and a ca. 2-fold selectivity over BRD4(2) (KD=ca. 260 nm). Its binding to the first bromodomain of BRD4 was determined by a protein cocrystal structure

Nowy rodzaj tekstylnej linii transmisyjnej o impedancji 50 omów

The article describes a new kind of textile transmission line which might be widely used, among others, in smart clothing. This line consists of a textile substrate in the form of fabric, sewn with paths made from electrically conductive fabric. The arrangement of electrically conductive paths in the line proposed makes it easy to obtain the possibility of impedance changes in a wide range of its values (potentially giving the possibility to obtain an impedance equal to 50 Ω), regardless of the type of fabric used as a substrate. The paper presents examples of measurement results of characteristic impedance of the line affecting its transmission properties. It also presents an impact study of the type of stitches fastening electrically conductive paths and the inaccurate creation of the line on the value of the characteristic impedance. The studies presented are based on real measurements and computer simulation using CST Studio software.W artykule opisano nowy rodzaj tekstylnej linii transmisyjnej mogącej znaleźć szerokie zastosowanie w tekstronice. Linia ta składa się z tekstylnego podłoża, na które naszyto ścieżki wykonane z tkaniny elektroprzewodzącej. Rozmieszczenie ścieżek elektroprzewodzących w proponowanej linii umożliwia łatwe uzyskanie impedancji równej 50 Ω, niezależnie od rodzaju tkaniny użytej na jej podłoże. W artykule przedstawiono wyniki pomiarów impedancji mającej wpływ na właściwości transmisyjne linii. Przedstawiono badania wpływu sposobu przyszycia ścieżek elektroprzewodzących linii oraz błędów jej wykonania na wartość jej impedancji

Pressure-volume method based porosimeter

W referacie przedstawiono konstrukcje przyrządu do pomiaru porowatości zbudowanego w ramach Grantu Promotorskiego. Przedstawiono zasadę działania przyrządu, która wykorzystuje prawa przemian gazowych. Zaprezentowano również wyniki wstępnych pomiarów cech metrologicznych przyrządu.In this paper the construction of porosimeter is presented. The porosimeter is based on the ideal gas law and is fully computerized and automated system. The system can measure the total volume, the total porosity, and the real density of sample. The results of initial measurements of the system are also presented

Stand for measurement of selected parameters of electrical transmission line

W artykule przedstawiono sposób wykonania pomiarów wybranych parametrów wysokoczęstotliwościowych linii transmisyjnych metodą reflektometrii w dziedzinie czasu z wykorzystaniem szybkiego oscyloskopu cyfrowego, generatora skoku jednostkowego oraz przyrządu wirtualnego wykonanego za pomocą programu LabVIEW. Przedstawiono teoretyczne podstawy metody reflektometrycznej. Podano wstępne, przykładowe pomiary wybranych parametrów elektrycznych testowanej linii transmisyjnej. Omówiono sposób oszacowania niepewności pomiaru jednego ze zmierzonych parametrów - prędkości propagacji.The paper presents a measuring stand for measurement of selected parameters of high frequency transmission lines by reflectometry method in time domain using a fast oscilloscope, voltage step generator and a virtual instrument constructed in LabVIEW. The theoret-ical foundations of the reflectometry method are presented. The paper describes a construction of stand for reflectometry measurement [Fig.2]. The stand uses a sampling oscilloscope, a fast voltage step generator and fast voltage divider. The paper also describes preliminary sample measurements of selected parameters of the tested electrical transmission line. Fig. 4 shows voltage wave, generated by voltage step generator, reflected from H155 coaxial cable with open, short circuit termination and terminated by 50 ? load. The paper also discusses, how to estimate the measurement uncertainty of the speed of propagation. As a result of first mea-surements it was found that the rise time value in the reflectometry system affects not only the resolution of the measurement results, but also the uncertainty propagation velocity and a dielectric constant of substrate of the transmission line. Fig.5 shows measurement of the pulse shape deviation from a rectangular. The proposed method is based on two measurements: tmin and tmax. These values determine the area of velocity results caused by deviation of the measured pulse from the rectangular shape. The table 1 shows uncertainty budget for cable of length 994 mm and tmin i tmax times for tested H155 cable. For measured times tmax i tmin the velocity of propagation Vpmin and Vpmax was computed. From equation (10) the combined uncertainty was computed. The results are presented in the table 2. The range comprising the true value of propagation velocity, according to the assumptions made, there is shown in Figure 6 (part A)