Cadmium in liver and kidneys of domestic Balkan and Alpine dairy goat breeds from Montenegro and Serbia

Concentrations of cadmium (Cd) were determined in the samples of 144 animals around 1 and of 144 animals around 4years old. Cd was analysed by inductively coupled plasma-optical emission spectrometry (ICP-OES), after microwave digestion. Cd concentrations were higher (p lt 0.05) in kidney than in liver and higher (p lt 0.05) in older animals than in young ones. In domestic Balkan goat which was raised in a free-ranged system Cd accumulation was lower (p lt 0.05) than in Alpine goat raised in an intensive production system. Geographic region did influence Cd accumulation only in older animals. Higher Cd levels (p lt 0.05) were determined in goats from Serbia. The highest obtained Cd concentrations in both tissues were lower than maximum levels set by European and national legislation for ruminants (cattle and sheep).This is the peer-reviewed version of the following article: Tomović, V.; Jokanović, M.; Tomović, M.; Lazović, M.; Šojić, B.; Škaljac, S.; Ivić, M.; Kocić-Tanackov, S.; Tomašević, I.; Martinović, A. Cadmium in Liver and Kidneys of Domestic Balkan and Alpine Dairy Goat Breeds from Montenegro and Serbia. Food Additives & Contaminants Part B-Surveillance 2017, 10 (2), 137–142. https://doi.org/10.1080/19393210.2017.1282987

Supplementary data for the article: Kecojević, I.; Đekić, S.; Lazović, M.; Mrkajić, D.; Baošić, R.; Lolić, A. Evaluation of LC-MS/MS Methodology for Determination of 179 Multi-Class Pesticides in Cabbage and Rice by Modified QuEChERS Extraction. Food Control 2021, 123, 107693. https://doi.org/10.1016/j.foodcont.2020.107693.

Supplementary material for: [https://doi.org/10.1016/j.foodcont.2020.107693]Related to published version: [https://cherry.chem.bg.ac.rs/handle/123456789/4433

Influence of selenium yeast on the growth, selenium uptake and mineral composition of Coriolus versicolor mushroom

The ability of Coriolus versicolor medicinal mushroom to grow and accumulate selenium during submerged cultivation in a selenium-fortified medium is examined in this paper. For selenium supplementation, commercial selenium yeast was used. Control, nonenriched sample and reference cultures cultivated in the medium enriched with commercial yeast Saccharomyces cerevisiae were also prepared. The mushroom demonstrated a high ability to accumulate selenium from the added source (around 970 and 1,300 mu g/g of dry mycelium weight for samples enriched with selenium in a concentration of 10 and 20 mg Se/L, respectively). The addition of selenium significantly (p lt = .05) increased the biomass yield, whereas the addition of nonenriched yeast had no significant (p lt = .05) impact. Furthermore, regression analysis showed statistically significant (p lt = .05) and positive correlations between the content of Se and Fe (r = .92), Se and Cu (r = .92), Se and Mn (r = .98), and Se and Sr (r = .96), suggesting that selenium incorporation was followed by incorporation of these elements, and led to mineral enrichment of the obtained mycelium. Methanol extracts prepared from mycelium biomass demonstrated a better inhibitory effect on Gram-positive bacterial strains with minimal inhibitory concentrations between lt 0.3125 and 40 mg/ml. The obtained results showed that selenium yeast could be used for obtaining a potential novel food supplement: mushroom biomass with high selenium content and enhanced mineral composition

Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism

Modern, efficient, and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4 center dot 5H(2)O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4 center dot 5H(2)O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contaminated environments

Cadmium and lead in female cattle livers and kidneys from Vojvodina, northern Serbia

Concentrations of cadmium (Cd) and lead (Pb) were determined in livers (n=52) and kidneys (n=52) of female cattle (345-2717days old) from dairy farms in the region Vojvodina. Cd and Pb were analysed by inductively coupled plasma-optical emission spectrometry, after microwave digestion. Cd and Pb concentrations did not exceed the Serbian and European maximum set limits in any sample. The Cd concentrations in the livers and kidneys ranged from 0.033 to 0.151mgkg(-1) wet weight and from 0.055 to 0.510mgkg(-1) wet weight, respectively. The corresponding Pb concentrations were 0.015-0.159mgkg(-1) wet weight and 0.021-0.196mgkg(-1) wet weight, respectively. Mean Cd and Pb concentrations were significantly lower (p lt 0.001) in the liver (0.072 and 0.053mgkg(-1) wet weight) than in the kidney (0.190 and 0.075mgkg(-1) wet weight). There were good correlations between Cd in liver and Cd in kidney, Pb in liver and Pb in kidney, Cd level and age and Pb level and age in both tissues