Raman spectroscopy of pharmacologically active compounds and biocatalysts

U ovoj disertaciji Ramanova spektroskopija korišćena je za sveobuhvatnu analizu primarnih mezenhimskih matičnih ćelija, poreklom iz periodoncijuma, mekog vezivnog tkiva koje spaja koren zuba za alveolarnu kost i ima ulogu u održavanju mineralizacije i ublažavaanja sila koje se javljaju žvakanjem...In this dissertation, the utilization of Raman spectroscopy for comprehensive analysis of primary mesenchymal stem cells from periodontal ligament, a soft connective tissue which anchors tooth root for the alveolar bone, was demonstrated. Since every cell has the unique Raman spectrum, Raman spectroscopy was used with the purpose to gain the insight in intrinsic biochemical prole on a single cell level. Mesenchymal stem cells from periodontal ligament were stimulated to dierentiate towards osteogenic, chondrogenic, and adipogenic lineages. These dierentiated cells were investigated by means of Raman spectroscopy as well. Microscopic glass cover-slips were used as substrates due to its cost-eectiveness and common use in laboratories, which is important for its clinical application. On the other hand, glass has strong Raman response, but it was demonstrated that the analysis was possible by taking into account certain spectral region in which its inuence was eliminated. In all sample groups, the same type of behavior was noticed, manifested as the increase of relative intensity Raman band of proteins/lipids, and the decrease of relative intensity of nucleic acids Raman bands. Further, the extensive statistical analysis in the form of principal component analysis was performed, revealing signi- cant groupings of cells with the same spectral features, which conrmed previously obtained results. Despite the heterogeneity of primary mesenchymal stem cells and their dierentiated lineages, it was proved that micro-Raman spectroscopy could adequately distinguish cell status, which is important for its clinical and medical use..

The possibility of in vitro multi-enzymatic method application for the assessment of the influence of thermal treatments on organic matter digestibility of feed for ruminants

In vitro multi-enzymatic method is a two-step procedure that uses exogenous enzymes for incubation of feed that aim to imitate digestive processes in the animal. It is used for determination of organic matter digestibility (OMD) of various feedstuffs and complete mixtures used in ruminant nutrition. The aim of the present work was to determine whether this in vitro multi-enzymatic method can be applied for the assessment of the influence of thermal treatments on OMD of feed for ruminants. Samples of corn and complete mixture for cows (based on corn) were subjected to pelleting, steam flaking, micronization and extrusion. Statistical analysis of the results did not show significant increase of corn and complete mixture OMD after various thermal treatments compared to untreated samples, which can be explained by high digestibility of corn itself (exceeds 90%). Based on obtained results it can be concluded that applied in vitro method is not suitable for the assessment of the influence of thermal treatments on OMD of corn and corn based mixture, while future research should be broadened to analysis of samples with lower OMD, such as sorghum, oats and barley

Changes in milk composition of domestic Balkan donkeys’ breed during lactation periods

Changes in the milk composition during early, middle and late stage of lactation period were monitored in 10 individual autochthonous donkeys of the Domestic Balkan breed. Animals were grazing ad libitum during early and middle lactation, while supplements (meadow hay, corn and corn stalks) were added during the late lactation period. The aim of this study was to evaluate the main compounds of donkeys’ milk during different periods of lactation. Based on the obtained results it was noticed that nutritional effects on milk composition were the greatest in the early and middle lactation. Chemical analysis indicated that donkeys’ milk was quite poor in dry matter (8.82-9.68%), fat (0.54-0.67%) and protein (1.44-1.79%), and conversely was high in lactose content (6.06-7.12%). Concentration of vitamin C varied significantly (P<0.05) during the lactation period and was very high (12.84-26.89 μg/mL) in comparison to milk from other animal species. Likewise, the content of lysozyme (1.95-3.29 g/L) and lactoferrin (1.56-3.14 g/L) during the early and middle lactation period showed an increase. Donkeys' milk did not show any significant differences in MUFA, n6-PUFA and UFA contents, while n3-PUFA, n6/n3 and UFA/SFA ratio showed a significant variability (P<0.05) during the lactation period.[Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III46012

Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations

Supplementary data 1: Table 1S. The chromatographic gradient profile; Table 2S. CPF concentration decrease (corresponding initial CPF concentration decrease was set as 0%) for all three forms of CPF depending on irradiation time; Table 3S. CPF and CPO concentrations determined chromatographically for TCPF, EW and EC formulations, as the function of irradiation time; % of CPO comparing to initial CPF concentration in all three forms of CPF; Supplementary data 2: Material safety data sheet according to 1907/2006/EC, Article 31/version 1; Supplementary data 3: Material safety data sheet according to 1907/2006/EC, Article 31/version 4Supplementary data for the article: [http://dx.doi.org/10.1016/j.foodchem.2018.07.207]Related to: [http://vinar.vin.bg.ac.rs/123456789/7821