4 research outputs found
ΠΡΠΊΡΠΎΡ Π²ΠΈΠ»ΡΠΎΠ²Ρ Π²Π»Π°ΡΡΠΈΠ²ΠΎΡΡΡ ΠΠΠ-ΠΏΠΎΠ»ΡΠΌΠ΅ΡΠ½ΠΈΡ ΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΡΠ² Π· ΡΠ΅Π³ΡΠ΅Π³ΠΎΠ²Π°Π½ΠΎΡ ΠΏΡΠΎΠ²ΡΠ΄Π½ΠΎΡ ΠΌΠ΅ΡΠ΅ΠΆΠ΅Ρ
Π£ ΡΠΎΠ±ΠΎΡΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΈ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π΅Π»Π΅ΠΊΡΡΠΎΠ΄ΠΈΠ½Π°ΠΌΡΡΠ½ΠΈΡ
Ρ Π΅ΠΊΡΠ°Π½ΡΡΡΠΈΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ Π² Π΄ΡΠ°ΠΏΠ°Π·ΠΎΠ½Ρ 40-60 ΠΠΡ ΠΏΠΎΠ»ΡΠΌΠ΅ΡΠ½ΠΈΡ
ΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΡΠ² Π· Π³ΡΠ°ΡΡΡΠΎΠ²ΠΈΠΌΠΈ Π½Π°Π½ΠΎΠΏΠ»Π°ΡΡΠΈΠ½ΠΊΠ°ΠΌΠΈ Ρ Π΄Π²ΠΎΠΌΠ° ΡΡΠ·Π½ΠΈΠΌΠΈ ΡΠΈΠΏΠ°ΠΌΠΈ ΠΏΠΎΠ»ΡΠΌΠ΅ΡΡ. Π―ΠΊ ΠΏΠΎΠ»ΡΠΌΠ΅ΡΠ½Ρ ΠΌΠ°ΡΡΠΈΡΡ Π²ΠΈΠΊΠΎΡΠΈΡΡΠΎΠ²ΡΠ²Π°Π»ΠΈ Π½Π°Π΄Π²ΠΈΡΠΎΠΊΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΈΠΉ ΠΏΠΎΠ»ΡΠ΅ΡΠΈΠ»Π΅Π½ ΡΠ° ΠΏΠΎΠ»ΡΠ°ΠΌΡΠ΄ ΠΠ΅ΠΉΠ»ΠΎΠ½ 12.
ΠΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΠΎ ΠΎΠ΄Π½ΠΎΡΡΠ΄Π½ΡΡΡΡ Π³ΡΠ°Π½ΡΠ»ΠΎΠΌΠ΅ΡΡΠΈΡΠ½ΠΎΠ³ΠΎ ΡΠΊΠ»Π°Π΄Ρ ΠΏΠΎΠ»ΡΠΌΠ΅ΡΡ Π² ΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΡ ΡΠΈΠ»ΡΠ½ΠΎ Π²ΠΏΠ»ΠΈΠ²Π°Ρ Π½Π° Π²Π΅Π»ΠΈΡΠΈΠ½Ρ ΠΏΠ΅ΡΠΊΠΎΠ»ΡΡΡΠΉΠ½ΠΎΠ³ΠΎ ΠΏΠΎΡΠΎΠ³Ρ Π΅Π»Π΅ΠΊΡΡΠΎΠΏΡΠΎΠ²ΡΠ΄Π½ΠΎΡΡΡ ΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΡΠ², Π° ΡΠ°ΠΌΠ΅: ΡΠΈΠΌ ΠΌΠ΅Π½ΡΠ° Π΄ΠΈΡΠΏΠ΅ΡΡΡΡ ΡΠΎΠ·ΠΌΡΡΡΠ² ΡΠ°ΡΡΠΈΠ½ΠΎΠΊ,
ΡΠΈΠΌ Π½ΠΈΠΆΡΠΈΠΉ ΠΏΠΎΡΡΠ³ ΠΏΠ΅ΡΠΊΠΎΠ»ΡΡΡΡ. ΠΠ°Π»Π΅ΠΆΠ½ΠΎΡΡΡ Π΄ΡΠ΅Π»Π΅ΠΊΡΡΠΈΡΠ½ΠΎΡ ΠΏΡΠΎΠ½ΠΈΠΊΠ½ΠΎΡΡΡ Π²ΡΠ΄ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΡΡ Π½Π°ΠΏΠΎΠ²Π½ΡΠ²Π°ΡΠ° Π΄ΠΎΡΠ»ΡΠ΄ΠΆΡΠ²Π°Π½ΠΈΡ
ΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΡΠ² Π½Π΅ ΠΌΠ°ΡΡΡ ΠΏΠ΅ΡΠΊΠΎΠ»ΡΡΡΠΉΠ½ΠΎΡ ΠΏΠΎΠ²Π΅Π΄ΡΠ½ΠΊΠΈ Ρ ΠΌΠ°ΠΉΠΆΠ΅ Π½Π΅ Π·Π°Π»Π΅ΠΆΠ°ΡΡ Π²ΡΠ΄ ΡΠ°ΡΡΠΎΡΠΈ ΠΠΠ ΡΠ° ΡΠ°Π΄ΡΡΡΠ°
ΠΏΠΎΠ»ΡΠΌΠ΅ΡΠ½ΠΈΡ
Π³Π»ΠΎΠ±ΡΠ». ΠΡΠΈ ΡΡΠΎΠΌΡ ΠΎΠ΄Π½ΠΎΡΡΠ΄Π½ΡΡΡΡ Π³ΡΠ°Π½ΡΠ»ΠΎΠΌΠ΅ΡΡΠΈΡΠ½ΠΎΠ³ΠΎ ΡΠΊΠ»Π°Π΄Ρ ΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½ΠΎ Π²ΠΏΠ»ΠΈΠ²Π°Ρ Π½Π° Π·Π±ΡΠ»ΡΡΠ΅Π½Π½Ρ
ΠΏΠΎΠ³Π»ΠΈΠ½Π°Π½Π½Ρ Π΅Π»Π΅ΠΊΡΡΠΎΠΌΠ°Π³Π½ΡΡΠ½ΠΈΡ
Ρ
Π²ΠΈΠ»Ρ Ρ ΠΌΠ°ΡΠ΅ΡΡΠ°Π»Ρ, ΡΠΎ ΠΏΡΠ΄ΡΠ²Π΅ΡΠ΄ΠΆΡΡΡΡΡΡ Π±ΡΠ»ΡΡ Π²ΠΈΡΠΎΠΊΠΈΠΌΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ°ΠΌΠΈ
ΠΏΠΎΠ³Π»ΠΈΠ½Π°Π½Π½Ρ Ρ ΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΡΠ² Π½Π° ΠΎΡΠ½ΠΎΠ²Ρ Π½Π΅ΠΉΠ»ΠΎΠ½Ρ ΠΏΠΎΡΡΠ²Π½ΡΠ½ΠΎ Π· ΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ°ΠΌΠΈ Π· ΠΏΠΎΠ»ΡΠ΅ΡΠΈΠ»Π΅Π½ΠΎΠΌ.This work presents the results of investigation of the electrodynamic and shielding characteristics in
the range of 40-60 GHz of polymer composites with graphite nanoplatelets and two different types of polymers. Ultra-high molecular weight polyethylene and Nylon 12 polyamide were used as a polymer matrix. It
was found that the uniformity of the polymer particle size distribution in a composite strongly affects the
value of the percolation threshold of the electrical conductivity of composites, namely, the lower the particle size dispersion, the lower the percolation threshold. The dielectric permittivity dependences on the filler concentration of investigated composites do not have percolation behavior and are almost independent of
the electromagnetic radiation (EMR) frequency and the radius of polymer globules. At the same time, the
uniformity of the particle size distribution has a positive effect on the increase in the absorption of electromagnetic waves in the material, which is confirmed by higher absorption characteristics in nylon-based
composites compared to composites with polyethylene
The role of beneficial bacteria wall elasticity in regulating innate immune response
Background: Probiotics have great potential to contribute to development of healthy dietary regimes, preventive
care, and an integrated approach to immunity-related disease management. The bacterial wall is a dynamic entity,
depending on many components and playing an essential role in modulating immune response. The impact of cell
wall elasticity on the beneficial effects of probiotic strains has not been sufficiently studied.
The aim was to investigate the effect of lactic acid bacteria (LAB) and bifidobacteria strains on phagocytic system
cells (macrophages) as related to bacterial wall elasticity, estimated using atomic force microscopy (AFM).
Methods: We conducted studies on Balb/c line mice 18\u201320 g in weight using lyophilized strains of LAB\u2014Lactobacillus
acidophilus IMV B-7279, Lactobacillus casei IMV B-7280, Lactobacillus delbrueckii subsp. bulgaricus IMV B-7281, and
bifidobacteria\u2014Bifidobacterium animalis VKL and Bifidobacterium animalis VKB. We cultivated the macrophages obtained
from the peritoneal cavity of mice individually with the strains of LAB and bifidobacteria and evaluated their effect on
macrophages, oxygen-dependent bactericidal activity, nitric oxide production, and immunoregulatory cytokines. We used
AFM scanning to estimate bacterial cell wall elasticity.
Results: All strains had a stimulating effect on the functional activity of macrophages and ability to produce NO/NO2
in vitro. Lactobacilli strains increased the production of IL-12 and IFN-\u3b3 in vitro. The AFM demonstrated different cell wall
elasticity levels in various strains of LAB and bifidobacteria. The rigidity of the cell walls among lactobacilli was distributed
as follows: Lactobacillus acidophilus IMV B-7279 > Lactobacillus casei IMV B-7280 > Lactobacillus delbrueckii subsp.
bulgaricus IMV B-7281; among the strains of bifidobacteria: B. animalis VKB > B. animalis VKL. Probiotic strain survival in the
macrophages depended on the bacterial cell wall elasticity and on the time of their joint cultivation.
Conclusion: LAB and bifidobacteria strains stimulate immune-modulatory cytokines and active oxygen and nitrogen
oxide compound production in macrophages. Strains with a more elastic cell wall according to AFM data demonstrated
higher resistance to intracellular digestion in macrophages and higher level of their activation.
AFM might be considered as a fast and accurate method to assess parameters of probiotic strain cell wall to predict their
immune-modulatory properties