A Music Concert with Kimberly Wright and Mark H. Lawrence: A Guest Artist Performance

This is the program for the guest artist performance of Kimberly Wright, on the horn, and Mark H. Lawrence, on the trombone. The guest artists were accompanied by Dr. Russell Hodges on piano. The concert was held on April 8, 1994, in the Recital Hall of the Mabee Fine Arts Center

Kimberly Whatley and Lawrence Harrison, Jr. in a Joint Senior Recital

This is the program for the joint senior voice recital of soprano Kimberly Carol Whatley and tenor Lawrence Wayne Harrison, Jr. Pianist Jay Crowder assisted Harrison. The recital took place on February 2, 1989, in the Mabee Fine Arts Center Recital Hall

Yield and Woody Biomass Traits of Novel Shrub Willow Hybrids at Two Contrasting Sites

Shrub willow has great potential as a dedicated bioenergy crop, but commercialization and adoption by growers and end-users will depend upon the identification and selection of high-yielding cultivars with biomass chemistry and quality amenable to conversion to biofuels and bioenergy. In this study, critical traits for biomass production were evaluated among new genotypes of shrub willow produced through hybrid breeding. This study assessed the variation in yield, pest and disease resistance, biomass composition, and wood density in shrub willow, as well as the impact of genotypic and environmental factors on these particular phenotypes. Analysis of clonal genotypes established on two contrasting sites in New York State, Tully and Belleville, showed statistical differences by site for all of the traits. The greatest yield was observed at Belleville, NY, for two cultivars, ‘Fish Creek' (41Mg ha−1) and ‘Onondaga' (40Mg ha−1). Yields of Salix eriocephala genotypes were lowest, and they displayed susceptibility to rust and beetle damage. Variation in cellulose content in the stem biomass was controlled by environmental factors, with the majority of the genotypes displaying greater cellulose content at Belleville compared with Tully. In contrast, wood density was significantly greater at Tully than Belleville, and cellulose content was correlated with wood density. There were no significant correlations between biomass yield and density or any of the composition traits. These trials demonstrate that new genotypes produce improved yield and pest and disease resistance, with diverse compositional traits that can be matched with conversion technologie

Correlations of expression of cell wall biosynthesis genes with variation in biomass composition in shrub willow ( Salix spp.) biomass crops

We have measured significant genetically determined variation in biomass composition among breeding populations of shrub willow, a biomass feedstock crop. This project was aimed to ask whether patterns of cell wall gene expression can be correlated with genetic variation in biomass composition at harvest, in order to develop assays of early differences in gene expression as indicators of harvestable biomass chemical composition and potentially reduce the time of selection for new willow genotypes. Previous studies have demonstrated that manipulation of expression of cell wall biosynthetic genes results in altered biomass chemical composition. We analyzed genes encoding enzymes involved in lignin biosynthesis and carbohydrate active enzymes selected based on their functional characterization and conservation in Populus trichocarpa and Arabidopsis thaliana. Fragments of 20 genes were cloned from young stem cDNA of Salix sachalinensis and Salix miyabeana. Expression profiling in willow stem apical tissue and developing stem tissue was performed for each isolated gene using probe-based quantitative real-time PCR. Two willow parental genotypes and six progeny within a hybrid family were selected for analysis, and significant differences in expression among the individuals and between tissue types were observed for most of the genes. Significant correlations between patterns of gene expression and variation in the biomass chemical composition of those genotypes provide insight into the genetic regulation of lignocellulosic deposition in this important bioenergy crop and could be utilized as a tool for early selection of new genotype

Willow Cultivars

The State University of New York College of Environmental Science and Forestry (SUNY-ESF) presents fact sheets on Salix purpurea ‘Allegany’, Salix sachalinensis × S. miyabeana ‘Canastota’, Salix purpurea ‘Fish Creek’, Salix purpurea × S. miyabeana ‘Millbrook’, Salix purpurea × S. miyabeana ‘Oneida’, Salix purpurea ‘Onondaga’, Salix viminalis × S. miyabeana ‘Otisco’, Salix viminalis × S. miyabeana ‘Owasco’, Salix eriocephala ‘S25’, Salix caprea hybrid ‘S365’, Salix sachalinensis × S. miyabeana ‘Sherburne’, Salix × dasyclados ‘SV1’, Salix sachalinensis ‘SX61’, Salix miyabeana ‘SX64’, Salix miyabeana ‘SX67’, Salix viminalis × S. miyabeana ‘Tully Champion’

A dataset for assessing temporal changes in gene expression during the aging process of adult \u3ci\u3eDrosophila melanogaster\u3c/i\u3e

A Drosophila melanogaster genome-wide transcriptome dataset is available for studies on temporal patterns of gene expression. Gene expression was measured using two-dye color oligonucleotide arrays derived from Version 2 of the Drosophila Genomics Resource Center. A total of 15,158 oligonucleotide probes corresponded to a high proportion of the coding genes in the genome. The source of the flies was a highly genetically heterogeneous population maintained in an overlapping generation population regime. This regime was designed to maintain life history traits so that they were similar to those found in natural populations. Flies collected for the cohorts were obtained in a short period of time in a carefully controlled manner before virgin females and males were allowed to mate. Mated females were introduced into two large population cages in unusually high numbers (approximately 12,000 per cage) for a Drosophila laboratory longevity study. Samples were taken weekly from each cohort for 11 weeks; only a small proportion of surviving flies were present at the last two collection time points and thus they were exceptionally old compared to those collected in early-tomidlife samples. The data set is useful for studies of temporal patterns of gene expression as flies age. The very large size of each cohort, and relatively frequent incidence of temporal samples, allows for a fine-scale study of gene expression from young to very old flies

A dataset for assessing temporal changes in gene expression during the aging process of adult \u3ci\u3eDrosophila melanogaster\u3c/i\u3e

A Drosophila melanogaster genome-wide transcriptome dataset is available for studies on temporal patterns of gene expression. Gene expression was measured using two-dye color oligonucleotide arrays derived from Version 2 of the Drosophila Genomics Resource Center. A total of 15,158 oligonucleotide probes corresponded to a high proportion of the coding genes in the genome. The source of the flies was a highly genetically heterogeneous population maintained in an overlapping generation population regime. This regime was designed to maintain life history traits so that they were similar to those found in natural populations. Flies collected for the cohorts were obtained in a short period of time in a carefully controlled manner before virgin females and males were allowed to mate. Mated females were introduced into two large population cages in unusually high numbers (approximately 12,000 per cage) for a Drosophila laboratory longevity study. Samples were taken weekly from each cohort for 11 weeks; only a small proportion of surviving flies were present at the last two collection time points and thus they were exceptionally old compared to those collected in early-tomidlife samples. The data set is useful for studies of temporal patterns of gene expression as flies age. The very large size of each cohort, and relatively frequent incidence of temporal samples, allows for a fine-scale study of gene expression from young to very old flies

Staphylococcus aureus immunodominant surface antigen B is a cell-surface associated nucleic acid binding protein

<p>Abstract</p> <p>Background</p> <p><it>Staphylococcus aureus </it>immunodominant surface antigen B (IsaB) elicits an immune response during septicemia and is generally classified as a virulence factor, but its biological function remains completely undefined. In an attempt to identify staphylococcal RNA-binding proteins, we designed an RNA Affinity Chromatography assay and subsequently isolated IsaB.</p> <p>Results</p> <p>Western analysis indicated that IsaB was both secreted and cell-surface associated. Gel Shift analysis confirmed the RNA binding activity but revealed that IsaB bound to any nucleic acid without sequence specificity. IsaB exhibited the highest affinity for double-stranded DNA followed by single-stranded DNA and RNA. Because extracellular DNA has been shown to play a role in biofilm formation, we investigated the biofilm-forming capacity of an isogenic <it>isaB </it>deletion mutant but we found that IsaB did not contribute to biofilm formation under any conditions tested.</p> <p>Conclusion</p> <p>IsaB is an extracellular nucleic acid binding protein, with little to no sequence specificity, but its role in virulence remains unclear.</p