A PI3K-mediated negative feedback regulates Drosophila motor neuron excitability

Negative feedback can act as a homeostatic mechanism to maintain neuronal activity at a particular specified value. At the Drosophila neuromuscular junction, a mutation in the type II metabotropic glutamate receptor gene (mGluRA) increased motor neuron excitability by disrupting an autocrine, glutamate-mediated negative feedback. We show that mGluRA mutations increase neuronal excitability by preventing PI3 kinase (PI3K) activation and consequently hyperactivating the transcription factor Foxo. Furthermore, glutamate application increases levels of phospho-Akt, a product of PI3K signaling, within motor nerve terminals in an mGluRA-dependent manner. In humans, PI3K and type II mGluRs are implicated in epilepsy, neurofibromatosis, autism, schizophrenia and other neurological disorders; however, neither the link between type II mGluRs and PI3K, nor the role of Foxo in the control of neuronal excitability, had been previously reported. Our work suggests that some of the deficits in these neurological disorders might result from disruption of glutamate-mediated homeostasis of neuronal excitability

Positioning the Destination Product-Can Regional Tourist Boards Learn from Private Sector Practice?

This article examines the role of positioning in the marketing of a tourism destination. The study focuses on the current positioning strategies pursued by the Regional Tourist Boards (RTBs) in England. A recent nationwide consumer research study into short holiday destination choice in the UK revealed that consumers were confused by the regional product message. The evidence suggests that current RTB positioning strategies are failing to keep pace with the constantly evolving needs of the consumer. This article explores the reasons for clearly positioning the destination product and suggests that, although RTBs could learn from marketing strategies employed in other sectors of the tourism industry, there are likely to be organisational and cultural barriers inhibiting this learning curve

Principals: Catalysts for promoting student leadership

The role of the principal is pivotal in the development of student leadership within schools. As well as assuming a heavy administrative workload and undertaking numerous complex and time-absorbing responsibilities, the principal plays a significant part in facilitating student leadership development initiatives—in essence, this person becomes the steward of student leadership. This stewardship most often takes one of two forms: through direct or indirect involvement. A principal advocating stewardship through direct involvement is personally engaged in leadership activities, and works closely with student leaders. By contrast, indirect involvement requires the principal to empower colleagues with the responsibility of personally engaging with leaders, and to be involved in the philosophical and organisational components of the leadership program. Both forms have merit. This article underscores the importance of including student leadership programs within schools and examines the manner in which the principal can engage directly or indirectly in developing student leadership

Broadband acoustic backscatter from crude oil under laboratory-grown sea ice

Author Posting. © Acoustical Society of America, 2016. This article is posted here by permission of Acoustical Society of America for personal use, not for redistribution. The definitive version was published in Journal of the Acoustical Society of America 140 (2016): 2274–2287, doi:10.1121/1.4963876.In ice-covered seas, traditional air-side oil spill detection methods face practical challenges. Conversely, under-ice remote sensing techniques are increasingly viable due to improving operational capabilities of autonomous and remotely operated vehicles. To investigate the potential for under-ice detection of oil spills using active acoustics, laboratory measurements of high-frequency, broadband backscatter (75–590 kHz) from crude oil layers (0.7–8.1 cm) under and encapsulated within sea ice were performed at normal and 20 incidence angles. Discrete interfaces (water-oil, oil-ice, and ice-oil) are identifiable in observations following oil injections under the ice and during the subsequent encapsulation. A one-dimensional model for the total normal incidence backscatter from oil under ice, constrained by oil sound speed measurements from 10 C to 20 C and improved environmental measurements compared to previous studies, agrees well with preencapsulation observations. At 20 incidence angles echoes from the ice and oil under ice are more complex and spatially variable than normal incidence observations, most likely due to interface roughness and volume inhomogeneities. Encapsulated oil layers are only detected at normal incidence. The results suggest that high-frequency, broadband backscatter techniques may allow under-ice remote sensing for the detection and quantification of oil spills.Funding for this research was provided by the International Oil and Gas Producers Arctic Oil Spill Technology Joint Industry Programme under Contract No. 28-13-14. C.B. was supported by the WHOI Postdoctoral Scholar Program with funding from the United States Geological Survey. A.C.L. was supported in part by the Ocean Acoustics Program at the Office of Naval Research

Measuring orbital angular momentum superpositions of light by mode transformation

We recently reported on a method for measuring orbital angular momentum (OAM) states of light based on the transformation of helically phased beams to tilted plane waves [Phys. Rev. Lett.105, 153601 (2010)]. Here we consider the performance of such a system for superpositions of OAM states by measuring the modal content of noninteger OAM states and beams produced by a Heaviside phase plate

Evaluation of an acoustic remote sensing method for frontal-zone studies using double-diffusive instability microstructure data and density interface data from intrusions

© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Methods in Oceanography 17 (2016): 264-281, doi:10.1016/j.mio.2016.09.004.Understanding intrusive exchange at oceanic water mass fronts may depend on building data-constrained models of the processes, but obtaining the needed representative and comprehensive data is challenging. Acoustic imaging (remote sensing) is an attractive method for mapping the three-dimensional intrusion geometry to enable the required focused in situ sampling of the mixing processes in intrusions. The method depends on backscatter of sound from sharp interfaces and from microstructure resulting from double-diffusive instability (DDI), a probable occurrence at intrusions. The potential of the method is evaluated using data collected using established methods in a field of intrusions south of New England. Above and beneath warm and salty intrusions may lie diffusive–convective DDI microstructure and salt-fingering microstructure, respectively, marking the intrusion boundaries, providing the backscattering features. The data show that both types of microstructure can occur in close proximity within intrusions, but the question of whether this is common or not is unanswered by the modest amount of data, as are questions about continuity of DDI-microstructure in intrusions (to facilitate intrusion acoustic imaging) and variability of DDI-driven heat, salt and buoyancy fluxes. Analysis here shows that detectable backscatter from DDI-microstructure will occur, and can be easily measured when plankton scattering is low enough. Interface scattering is also likely to be detectable. The DDI-linked microstructure data used here are inherently interesting in their own right and are presented in some detail.The data were collected under Office of Naval Research grant N00014-03-1-0335. Acoustic analysis was done under grant N00014-14-1-0223/N00014-16-1-2372

Wideband (15–260 kHz) acoustic volume backscattering spectra of Northern krill (Meganyctiphanes norvegica) and butterfish (Peprilus triacanthus)

This paper is not subject to U.S. copyright. The definitive version was published in ICES Journal of Marine Science 74 (2017): 2249–2261, doi:10.1093/icesjms/fsx050.Measurements of acoustic backscatter made over a wide frequency band have the potential for improved classification relative to traditional narrowband methods, by characterizing more fully the frequency response of scatterers. In January 2014, five wideband transceivers [Simrad EK80 Wideband Transceivers (WBTs)] and split-beam transducers with nominal centre frequencies of 18, 38, 70, 120, and 200 kHz were used to collect acoustic data spanning a nearly continuous 15–260 kHz bandwidth. The acoustic samples were from ca. 2 m below the surface to the seabed in an area along the US continental shelf break. Bottom trawls and zooplankton nets were also used to sample scatterers contributing to selected features of the acoustic backscatter. Measurements of frequency-dependent volume backscattering strength (i.e. volume backscattering spectra) from aggregations of euphausiids (mostly Northern krill, Meganyctiphanes norvegica) clearly resolved the transition from Rayleigh to geometric scattering, consistent with modelled backscatter from the type and length of animals sampled with bongo nets. Volume backscattering spectra from aggregations dominated by butterfish (Peprilus triacanthus) revealed a frequency response that was suggestive of superimposed scattering by soft tissue and bone. Backscatter predicted by Kirchhoff ray mode models of butterfish corresponded to trends in the measured spectra, supporting the assumption that acoustic scattering by butterfish is dominated by soft tissue and vertebrae.NOAA Advanced Sampling Technology Working Group (ASTWG) provided support for this project. GLL was partially supported by NOAA Cooperative Agreements NA09OAR4320129 and NA14OAR4320158 through the NOAA Fisheries Quantitative Ecology and Socioeconomics Training (QUEST) programme