Table S3. Trout sex database

CSV file with parental traits and family data on survival and sex ratio

Rainbow trout (Oncorhynchus mykiss) skin digital images

160 rainbow trout were fed either a commercial based diet (80 fish) or a 100 % plant-based diet (80 fish) and photographed using consumer-grade digital camera. Each live fish was photographed with a 12-megapixel Nikon D3300 digital camera (Nikon Corp., Tokyo, Japan) under a lighting system consisting of four halogen lamps (200 W bulb) at an angle of 45 degrees, and 35 cm above the sample to not only provide constant intensity output but also to give the uniform light intensity over the fish sample. Images were collected in a dark room with only light source, coming through halogen lamps cast on fish skin. Digital camera was located vertically at 56 cm from the sample. All setting on the camera were on manual. The setting of camera was: exposure mode = manual, shutter speed = 1/160 s, aperture = f/4.0, ISO sensitivity = 100. The images were recorded in Nikon Raw format (NEF) and transferred to a laptop for further processing.<div>An excel sheet which includes all relevent details also provided. </div

Principal component analysis.

<p>(A) 10 lines for CV body weight, average body weight, condition factor, risk taking behavior and cortisol levels after confinement challenges; (B) 7 lines for CV body weight, average body weight, condition factor, risk taking behavior, spatial exploration and flight response traits, and cortisol levels after confinement challenges. Variables with cos2 < 0.5 are shown in grey with no labels (the cos2 values are used to estimate the quality of the representation for variables on the factor map).</p

The changes of mean body weights and coefficient of variation (CV) for body weight (mean ± SEM, in %) over the period of experiment.

<p>Each dot represents the mean of three replicates. SEM: standard error of the mean.</p

Ingredients and proximal composition of the experimental diets M (M1, M2) and PB (PB1, PB2).

1<p>M1 diet was given from first feeding to 236 dpf and M2 from 236 dpf until the end of the trial to adapt protein and lipid content to the nutritional requirements of larger fish.</p>2<p>PB1 diet was given from first feeding to 280 dpf and PB2 from 280 dpf until the end of the trial to adapt protein and lipid content to the nutritional requirements of larger fish.</p

Ingredients, proximal composition of the experimental diets V-1 [27], V, and M, and fatty acid composition of the M and V (2mm) experimental diets (DM: dry matter).

<p>Ingredients, proximal composition of the experimental diets V-1 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0186705#pone.0186705.ref027" target="_blank">27</a>], V, and M, and fatty acid composition of the M and V (2mm) experimental diets (DM: dry matter).</p

Growth and survival performance.

<p>(a) Body weights and (b) survival rate of the control (SY) and selected (SU) fish fed the M diet or the V diet (SY-M, SU-M, SY-V, SY-M) obtained for the period 1 and period 2.</p

Results of the statistical analysis on apparent digestibility.

<p>Results of the statistical analysis on apparent digestibility.</p

Performance comparison between the first and third generation of selection.

<p>Evolution of (a) mean body weights and (b) survival between the first and the third generation of selection for the 4 different conditions (SU-M, SY-M, SU-V and SY-V), at 193 dpf for the first generation, and 197 dpf for the third one.</p

Results of the statistical analysis on composition of whole fish and nutrient retention.

<p>Results of the statistical analysis on composition of whole fish and nutrient retention.</p