Body mass index and blood pressure in a semi-urban community in Ota, Nigeria

This study was designed to establish the relationship between body mass index (BMI) and blood pressure (BP) in an increasingly industrialised town in Nigeria due to the rising prevalence of hypertension in non-industrialised countries. Factors associated with BMI and BP levels were determined in three hundred adult male and female subjects in Ota community of Ogun State, Nigeria. The levels of the overweight among the male and female subjects were 53.03 % and 47.37 % respectively. The levels of hypertensive male and female subjects were 40.91 % and 35.34 % respectively. The overweight and underweight among the hypertensive male were 54.29 % and 0 % respectively; while the overweight and underweight among the hypertensive female were 42.86 % and 28.57 % respectively. Hypertension among the overweight, and hypotension among the underweight, are major health concern in Ota that requires intensive medical care

Additional file 2: of Genomic differentiation among wild cyanophages despite widespread horizontal gene transfer

Lineage information. Figure S2. TEM images of phage isolates from cyanophage (A) lineage I, (B) lineage II, (C) lineage III, (D) lineage IV, (E) lineage V, (F) lineage VI, and (G-J) singleton and duplicon populations confirms myovirus morphology. Table S2. List of 51 core protein clusters shared across all six phylogenetic lineages. Fig. S3. Unrooted phylogenomic maximum likelihood tree of 27 concatenated protein sequences shared across published marine and non-marine T4-like phage genomes and the 142 cyanophage isolate genomes sequenced here. For simplicity, cyanophage isolate names in this tree were shortened from Syn7803* to just *. These analyses show that the 10 cyanophage populations observed here share similar evolutionary histories with other T4-like phages. Table S3. Average ANI of the 51 core genes within and between lineages. Table S4. AGDB groupings correspond with the phylogenetic lineages. Table S5. Average phylogenetic distances within and between lineages. Table S6. Corrected Rand Indices and Malia’s VI values to compare the row and column hierarchical clustering between the original ANI and Shared Gene matrix and a randomized ANI and Shared Gene matrix, respectively. The hierarchical clusters were split into different number of clusters (5,10, 20 and 50) for the analyses. The analyses revealed low correspondence between clustering, indicating that the clustering we observe in the original matrices are not random. (DOC 4227 kb

Additional file 3: of Genomic differentiation among wild cyanophages despite widespread horizontal gene transfer

Testing genetic drift, selection, and recombination. Table S7. FST values for genetic differentiation between coastal and offshore populations within each cyanophage lineage. The FST value for lineage II is most likely high due to the low number of representatives and lack of diversity among the offshore lineage II phages. Due to the low number of individuals isolated from the offshore site for some of the clusters, FST could not be calculated. Figure S4. (A) Quantitative host range analyses of 15 Synechococcus host strains against 138 cyanophage isolates testing the efficacy of infection. (B) Analysis of mean infectivity of coastal and upwelling phages in lineage I, II, IV, and VI reveal statistically different infectivity phenotypes at either site with T-test p <0.05 (*). Statistical significance was not assessed for lineages III and V due to low sample size nor on the original isolation host, WH7803 (†). Table S8. Corrected Rand Indices and Malia’s VI values to hierarchical clustering between the original host range matrix and a randomized host range matrix. The hierarchical clusters were split into different number of clusters (5,10, 20 and 50) for the analyses. The analyses revealed low correspondence between clustering, indicating that the clustering we observe in the original shared genes matrix is not random and that there is some correlation with a biological signal. Table S9. Fisher’s exact tests p-values and phi coefficients (for effect size) for genes found under positive selection in comparisons between phylogenomic lineages using the non-polarized McDonald-Kreitman test. Also, the table reflects the corresponding protein clusters in the GOV population dataset [19]. Table S10. Genic versus intergenic recombination breakpoints for each lineage. (DOC 250 kb

Additional file 1: of Genomic differentiation among wild cyanophages despite widespread horizontal gene transfer

Metadata. Table S1. Metadata from the coastal (H3) and mesotrophic (67-70) sites. Samples for the 16S rRNA amplicons were collected twice at 67-70, 8 days apart (with the 10 Oct. sample being done on the same day as the viral sample). The mesotrophic station is often subject to upwelling. Figure S1. Sea surface temperatures (SST) of the region of the California Cooperative Oceanic Fisheries Investigations (CalCOFI) Line 67 ocean transect on 5 October 2009 (contoured from a single synoptic image, Aqua Modis, NOAA) with the locations of the nearshore (yellow, H3 - coastal) and offshore (red, 67-70 - offshore mesotrophic) stations marked with stars. Gene marker (16S rRNA gene amplicons) analyses using the reference alignments of ref. Sudek et al., 2015 revealed different Synechococcus communities at the two sites (for additional details on sampling details see Additional file 2: Table S1). The Synechococcus community was analyzed twice at 67-70, one day after the coastal sampling and on the same day as the viral 67-70 sample collection. Proportions of different clades varied in the 67-70 Synechococcus amplicon data but the same clades were present on both dates. (DOC 721 kb

Additional file 4 of Microbiota Transfer Therapy alters gut ecosystem and improves gastrointestinal and autism symptoms: an open-label study

Supplementary text. (PDF 123 kb

Additional file 2: Dataset S1. of Microbiota Transfer Therapy alters gut ecosystem and improves gastrointestinal and autism symptoms: an open-label study

A summary on participants’ characteristics and their medical and diet history. (XLSX 53 kb