Micro-geophysics to assess the integrity of some statues in the Museo Egizio of Turin, Italy

On request of the Soprintendenza in charge of the Museo Egizio of Turin, a quite large number of tests have been performed on four statues of the museum to assess their integrity both for practical (moving the statue) and archaeological purposes. Ultrasonic tomography and georadar have been used with fine results on sub-decimeter scale. In this paper we present the main results on the statues of the Pharaons Ramses II (Fig.1 left) and Tuthmosis I. (Fig.2 left). Both the statues belong to the collection set up by Bernardino Drovetti, Console Generale of France in Egypt in the early XIX century. Ramses II statue was restored in the first half of the XIX century. Few documents can be found on these restoration works: very likely the statue arrived broken to Turin and was reassembled with cement mortar (Hartleben, 1909) No news can be found neither on the type of mortar nor on the quantity of mortar actually used. The statue of Ramses was probably found at Tebe in 1818. It is made by basanite exploited from a quarry in Uadi Hammamat. The basanite is a basaltic extrusive rock also known as Lydian stone or lydite. It was almost exclusively reserved to the crafting of statues of pharaons or divinities. In many parts of the statue the mortar (as dark as the stone) is clearly visible (Fig.1 right). The statue of Tutmosis I was found by J.J. Rifaud , a Drovetti 's agent, in 1818 in Tebe, very likely in the Karnak temple. The king sits on a throne with many scripts on both the sides of the seat. The statue is made by a dark diorite with some light pink plagioclase crystals sizing few centimeters. Its conservation seems fairly goo

P-wave and surface wave survey for permafrost analysis in alpine regions

We discuss the results of seismic survey to delineate temporal and spatial changes of frozen layer in Alpine environments. The seismic characterization allows us to detect changes of soil deformability properties related to the temperature effect on subsoi

Application and comparison of three tomographic techniques for detection of decay in trees

This paper reports application of electric, ultrasonic, and georadar tomography for detection of decay in trees and their comparison with the traditional penetrometer. Their feasibility in arboriculture is also evaluated, critically considering some "open problems." The experiments were carried out in an urban environment on two plane (Platanus hybrida Brot.) trees. Both trees, after felling, showed extensive white rot in the central cylinder. The electric tomography revealed low resistivity zones roughly centered in the trunk. A comparison with the successively cut sections showed a fine correspondence to decayed areas and a strong correspondence between high moisture zones and low resistivity zones. Ultrasonic tomography demonstrated to be a very effective tool for the detection of internal decay, accurately locating the position of the anomalies and estimating their size, shape, and characteristic in terms of mechanical properties. With the georadar technique, the high contrast of electromagnetic impedance measured between the inner decayed section and the outside sound section allowed the detection of the interface between the sound and decayed section of the tree, using radar acquisition in reflection modality. The penetrometer profiles detected the low-resistance areas inside the two trunk

Metodi geofisici per la caratterizzazione degli ammassi rocciosi

Vengono illustrati i risultati che si possono ottenere nello studio delle caratteristiche fisiche degli ammassi rocciosi con le diverse tecniche grofisich

Bone invading NSCLC cells produce IL-7: mice model and human histologic data

<p>Abstract</p> <p>Background</p> <p>Bone metastases are a common and dismal consequence of lung cancer that is a leading cause of death. The role of IL-7 in promoting bone metastases has been previously investigated in NSCLC, but many aspects remain to be disclosed. To further study IL-7 function in bone metastasis, we developed a human-in-mice model of bone aggression by NSCLC and analyzed human bone metastasis biopsies.</p> <p>Methods</p> <p>We used NOD/SCID mice implanted with human bone. After bone engraftment, two groups of mice were injected subcutaneously with A549, a human NSCLC cell line, either close or at the contralateral flank to the human bone implant, while a third control group did not receive cancer cells. Tumor and bone vitality and IL-7 expression were assessed in implanted bone, affected or not by A549. Serum IL-7 levels were evaluated by ELISA. IL-7 immunohistochemistry was performed on 10 human bone NSCLC metastasis biopsies for comparison.</p> <p>Results</p> <p>At 12 weeks after bone implant, we observed osteogenic activity and neovascularization, confirming bone vitality. Tumor aggressive cells implanted close to human bone invaded the bone tissue. The bone-aggressive cancer cells were positive for IL-7 staining both in the mice model and in human biopsies. Higher IL-7 serum levels were found in mice injected with A549 cells close to the bone implant compared to mice injected with A549 cells in the flank opposite to the bone implant.</p> <p>Conclusions</p> <p>We demonstrated that bone-invading cells express and produce IL-7, which is known to promote osteoclast activation and osteolytic lesions. Tumor-bone interaction increases IL-7 production, with an increase in IL-7 serum levels. The presented mice model of bone invasion by contiguous tumor is suitable to study bone-tumor cell interaction. IL-7 plays a role in the first steps of metastatic process.</p

A Mouse Model of Pulmonary Metastasis from Spontaneous Osteosarcoma Monitored In Vivo by Luciferase Imaging

BACKGROUND: Osteosarcoma (OSA) is lethal when metastatic after chemotherapy and/or surgical treatment. Thus animal models are necessary to study the OSA metastatic spread and to validate novel therapies able to control the systemic disease. We report the development of a syngeneic (Balb/c) murine OSA model, using a cell line derived from a spontaneous murine tumor. METHODOLOGY: The tumorigenic and metastatic ability of OSA cell lines were assayed after orthotopic injection in mice distal femur. Expression profiling was carried out to characterize the parental and metastatic cell lines. Cells from metastases were propagated and engineered to express Luciferase, in order to follow metastases in vivo. PRINCIPAL FINDINGS: Luciferase bioluminescence allowed to monitor the primary tumor growth and revealed the appearance of spontaneous pulmonary metastases. In vivo assays showed that metastasis is a stable property of metastatic OSA cell lines after both propagation in culture and luciferase trasduction. When compared to parental cell line, both unmodified and genetically marked metastatic cells, showed comparable and stable differential expression of the enpp4, pfn2 and prkcd genes, already associated to the metastatic phenotype in human cancer. CONCLUSIONS: This OSA animal model faithfully recapitulates some of the most important features of the human malignancy, such as lung metastatization. Moreover, the non-invasive imaging allows monitoring the tumor progression in living mice. A great asset of this model is the metastatic phenotype, which is a stable property, not modifiable after genetic manipulation