Missoula Greenhouse Gas Emissions Inventory and Analysis, 2003-2008: Toward A Blueprint For Municipal Sustainability

The City of Missoula has been a signer on the U.S. Conference of Mayors Climate Protection Agreement for three mayor administrations, with Mayor John Engen renewing the pledge shortly after he took office in 2006. Mayor Engen and the City of Missoula formed a partnership in January 2009 with The University of Montana and Professor Robin Saha of the Environmental Studies Program to complete an emissions inventory of municipal operations. The specific goals of the report are: 1. To present a baseline greenhouse gas emissions inventory for the City of Missoula that quantifies total energy use and associated emissions for municipal operations. 2. To identify major sources of municipal GHG emissions and relative contributions within and among the various sectors examined. 3. To analyze changes and trends in energy use, costs and emissions from Fiscal Years (FY) 2003 to 2008. 4. To identify opportunities and offer recommendations to achieve future municipal GHG emission reductions and energy cost savings

Reaction Rates Uncertainties and the Production of F19 in AGB Stars

We present nucleosynthesis calculations and the resulting 19F stellar yields for a large set of models with different masses and metallicity. We find that the production of fluorine depends on the temperature of the convective pulses, the amount of primary 12C mixed into the envelope by third dredge up and the extent of the partial mixing zone. Then we perform a detailed analysis of the reaction rates involved in the production of 19F and the effects of their uncertainties. We find that the major uncertainties are associated with the 14C(alpha,gamma)18O and the 19F(alpha,p)22Ne reaction rates. For these two reactions we present new estimates of the rates and their uncertainties. The importance of the partial mixing zone is reduced when using our estimate for the 14C(alpha,gamma)18O rate. Taking into account both the uncertainties related to the partial mixing zone and those related to nuclear reactions, the highest values of 19F enhancements observed in AGB stars are not matched by the models. This is a problem that will have to be revised by providing a better understanding of the formation and nucleosynthesis in the partial mixing zone, also in relation to reducing the uncertainties of the 14C(alpha,gamma)18O reaction rate. At the same time the possible effect of Cool Bottom Processing at the base of the convective envelope should be included in the computation of AGB nucleosynthesis. This process could in principle help matching the highest 19F abundances observed by decreasing the C/O ratio at the surface of the star, while leaving the 19F abundance unchanged.Comment: 40 pages, 8 figures, accepted for publication on the Astrophysical Journa

C and N Abundances in Stars At the Base of the Red Giant Branch in M15

We present an analysis of a large sample of moderate resolution Keck LRIS spectra of subgiants and stars at the base of the RGB in M15, most within the range 16.5 < V < 19.5 (1.2 < M_V < 4.2), with the goal of deriving C abundances (from the G band of CH) and N abundances (from the NH band at 3360 A). Star-to-star stochastic variations with significant range in both [C/Fe] and [N/Fe] are found at all luminosities extending to the subgiants at M_V ~ +3. The C and N abundances appear anti-correlated, as would be expected from the CN-cycle processing of stellar material. Yet these M15 stars are considerably fainter than the RGB bump, the point at which deep mixing is believed to set in. On this basis, while the observed abundance pattern is consistent with proton capture nucleosynthesis, we infer that the site of the reactions is likely not within the present sample. The range of variation of the N abundances is very large and the sum of C+N increases as C decreases. To reproduce this requires the incorporation not only of CN but also of ON-processed material. Combining our work with that of Trefzger et al (1983) for the brighter giants in M15, we find strong evidence for additional depletion of C among the most luminous giants. This presumably represents the first dredge up (with enhanced deep mixing) expected for such luminous cluster RGB stars in the course of normal stellar evolution as they cross the RGB bump. We compare the behavior of these patterns for C and N in globular clusters covering a wide range of metallicity and of current mass and discuss possible scenarios to reproduce the observed behavior of these key elements. (abridged)Comment: Submitted to the AJ Feb. 1, 2005. Accepted May 11, 2005. Delay due to tardy anonymous refere

Stable isotope dilution assay for the accurate determination of mycotoxins in maize by UHPLC-MS/MS

A fast, easy-to-handle and cost-effective analytical method for 11 mycotoxins currently regulated in maize and other cereal-based food products in Europe was developed and validated for maize. The method is based on two extraction steps using different acidified acetonitrile–water mixtures. Separation is achieved using ultrahigh-performance liquid chromatography (UHPLC) by a linear water–methanol gradient. After electrospray ionisation, tandem mass spectrometric detection is performed in dynamic multiple reaction monitoring mode. Since accurate mass spectrometric quantification is hampered by matrix effects, uniformly [13C]-labelled mycotoxins for each of the 11 compounds were added to the sample extracts prior to UHPLC-MS/MS analysis. Method performance parameters were obtained by spiking blank maize samples with mycotoxins before as well as after extraction on six levels in triplicates. The twofold extraction led to total recoveries of the extraction steps between 97% and 111% for all target analytes, including fumonisins. The [13C]-labelled internal standards efficiently compensated all matrix effects in electrospray ionisation, leading to apparent recoveries between 88% and 105% with reasonable additional costs. The relative standard deviations of the whole method were between 4% and 11% for all analytes. The trueness of the method was verified by the measurement of several maize test materials with well-characterized concentrations. In conclusion, the developed method is capable of determining all regulated mycotoxins in maize and presuming similar matrix effects and extraction recovery also in other cereal-based foods

Catching Element Formation In The Act

Gamma-ray astronomy explores the most energetic photons in nature to address some of the most pressing puzzles in contemporary astrophysics. It encompasses a wide range of objects and phenomena: stars, supernovae, novae, neutron stars, stellar-mass black holes, nucleosynthesis, the interstellar medium, cosmic rays and relativistic-particle acceleration, and the evolution of galaxies. MeV gamma-rays provide a unique probe of nuclear processes in astronomy, directly measuring radioactive decay, nuclear de-excitation, and positron annihilation. The substantial information carried by gamma-ray photons allows us to see deeper into these objects, the bulk of the power is often emitted at gamma-ray energies, and radioactivity provides a natural physical clock that adds unique information. New science will be driven by time-domain population studies at gamma-ray energies. This science is enabled by next-generation gamma-ray instruments with one to two orders of magnitude better sensitivity, larger sky coverage, and faster cadence than all previous gamma-ray instruments. This transformative capability permits: (a) the accurate identification of the gamma-ray emitting objects and correlations with observations taken at other wavelengths and with other messengers; (b) construction of new gamma-ray maps of the Milky Way and other nearby galaxies where extended regions are distinguished from point sources; and (c) considerable serendipitous science of scarce events -- nearby neutron star mergers, for example. Advances in technology push the performance of new gamma-ray instruments to address a wide set of astrophysical questions.Comment: 14 pages including 3 figure

Effects of Fe deficiency on the protein profiles and lignin composition of stem tissues from Medicago truncatula in absence or presence of calcium carbonate

12 Pags.- 2 Tabls.- 5 Figs.- Supp. Data.Iron deficiency is a yield-limiting factor with major implications for crop production, especially in soils with high CaCO3. Because stems are essential for the delivery of nutrients to the shoots, the aim of this work was to study the effects of Fe deficiency on the stem proteome of Medicago truncatula. Two-dimensional electrophoresis separation of stem protein extracts resolved 276 consistent spots in the whole experiment. Iron deficiency in absence or presence of CaCO3 caused significant changes in relative abundance in 10 and 31 spots, respectively, and 80% of them were identified by mass spectrometry. Overall results indicate that Fe deficiency by itself has a mild effect on the stem proteome, whereas Fe deficiency in the presence of CaCO3 has a stronger impact and causes changes in a larger number of proteins, including increases in stress and protein metabolism related proteins not observed in the absence of CaCO3. Both treatments resulted in increases in cell wall related proteins, which were more intense in the presence of CaCO3. The increases induced by Fe-deficiency in the lignin per protein ratio and changes in the lignin monomer composition, assessed by pyrolysis-gas chromatography–mass spectrometry and microscopy, respectively, further support the existence of cell wall alterations. Biological significance: In spite of being essential for the delivery of nutrients to the shoots, our knowledge of stem responses to nutrient deficiencies is very limited. The present work applies 2-DE techniques to unravel the response of this understudied tissue to Fe deficiency. Proteomics data, complemented with mineral, lignin and microscopy analyses, indicate that stems respond to Fe deficiency by increasing stress and defense related proteins, probably in response of mineral and osmotic unbalances, and eliciting significant changes in cell wall composition. The changes observed are likely to ultimately affect solute transport and distribution to the leaves.Work supported by the Spanish Ministry of Science and Competitiveness (MINECO; projects AGL2012-31988, AGL2011-25379 and AGL2013-42175-R, co-financed by FEDER), the Aragón Government (group A03), and the US Department of Agriculture, Agricultural Research Service (under Agreement number 58-6250-0-008 to MAG). Support was obtained by contracts I3P-CSIC (JRC), FPI-MINECO (GL and LC-L), JAE-PRE-CSIC (EG-C) and JAE-DOC-CSIC (JR), co-financed by the European Social Fund.Peer reviewe

Acute kidney injury: new concepts in definition, diagnosis, pathophysiology, and treatment.

Acute kidney injury (AKI) is increasingly recognized in all fields of medical practice. Unfortunately, this syndrome has been plagued by inconsistent definitions, simplistic pathophysiologic schemas, and insensitive diagnostic tools. Recent advances in defining AKI, understanding its pathophysiology, and improving the diagnostic accuracy of the testing tools available eventually will impact disease management and clinical outcomes. Prompt recognition and treatment of AKI remain the cornerstone of clinical management for this high-mortality, high-cost syndrome. The authors provide the most recent updates in the definition, diagnosis, pathophysiology, and treatment options for patients with AKI, providing a stepwise approach to clinical evaluation for use in all fields of medical practice

Assessment of the EUMETSAT Multi Decadal Land Surface Albedo Data Record from Meteosat Observations

Surface albedo, defined as the ratio of the surface-reflected irradiance to the incident irradiance, is one of the parameters driving the Earth energy budget and it is for this reason an essential variable in climate studies. Instruments on geostationary satellites provide suitable observations allowing long-term monitoring of surface albedo from space. In 2012, EUMETSAT published Release 1 of the Meteosat Surface Albedo (MSA) data record. The main limitation effecting the quality of this release was non-removed clouds by the incorporated cloud screening procedure that caused too high albedo values, in particular for regions with permanent cloud coverage. For the generation of Release 2, the MSA algorithm has been replaced with the Geostationary Surface Albedo (GSA) one, able to process imagery from any geostationary imager. The GSA algorithm exploits a new, improved, cloud mask allowing better cloud screening, and thus fixing the major limitation of Release 1. Furthermore, the data record has an extended temporal and spatial coverage compared to the previous release. Both Black-Sky Albedo (BSA) and White-Sky Albedo (WSA) are estimated, together with their associated uncertainties. A direct comparison between Release 1 and Release 2 clearly shows that the quality of the retrieval improved significantly with the new cloud mask. For Release 2 the decadal trend is less than 1% over stable desert sites. The validation against Moderate Resolution Imaging Spectroradiometer (MODIS) and the Southern African Regional Science Initiative (SAFARI) surface albedo shows a good agreement for bright desert sites and a slightly worse agreement for urban and rain forest locations. In conclusion, compared with MSA Release 1, GSA Release 2 provides the users with a significantly more longer time range, reliable and robust surface albedo data record