Presence of Circulating Anti-Myosin Antibodies in Endomyocardial Fibrosis

Endomyocardial Fibrosis is a tropical disease in which the heart cannot open properly to receive blood due to a scar that covers its inner layer. It affects mainly children and adolescents, and has a poor prognosis because the cause and mechanisms of scarring are unknown. The conventional treatment is frustrating and does not alter the natural history of the disease. Despite affecting several million people worldwide there has been little investigation on the mechanisms of the disease or drug development to improve its prognosis. In this study we investigate the presence of antibodies against the myocardial cells of African patients with severe and advanced EMF aiming at uncovering new pathways for the disease. Our results reveal that EMF patients have anti-myocardial antibodies in their blood. The reaction of these antibodies with the heart may be one of the mechanisms involved in the genesis of the fibrotic lesions. This knowledge may help in diagnosing the condition and provide alternatives for its management, using drugs that reduce the impact of the circulating antibodies in the cardiac tissue. The significance of these results needs confirmation on studies involving larger number of subjects due to frequent finding of antiheart antibodies in African populations with heart failure of any cause

Morphological characterization of potato (Solanum tuberosum L.) germplasm under rainfed environment

In this study, 11 potato cultivars were assessed based on their genetic variability of different morphological parameters, including emergence percentage (%), date of flowering, plant height (cm), number of leaves per plant, average leaf area, number of flowers per plant, number of stems per plant, and number of fruits per plant. All of these characters showed a high level of variation among all the varieties. Locally developed advanced line SH-19 showed maximum emergence percentage (92.33%). Among other characters, Asterix, Smeengi and SH-19 produced considerable amount of flowers per plant (37.39). Romeo touched the line, indicating three stems per plant and was grouped into a separate group ‘A’. Cultivars Smeengi and Aziza, Leonardo and Avalanche carried 2.67 and 1 stem per plant respectively. Moreover, the 11 cultivars were ranked and grouped into 5 groups (A to E). For number of fruits/plant, means were not very much different from one another. The pyramid of variety Asterix was the highest, indicating that this genotype is efficient in bearing fruits. Variation was observed for tuber yield among all the cultivars and was not significantly high between replications of all varieties. Overall, there was highly significant variation for this character among all the potato cultivars. All the 11 varieties were grouped into seven homogeneous groups (A to G) in which the means are not significantly different from one another. Almost every variety was in a different group. Average yield for all the varieties ranged from 22 to 7 kg. Pyramid height for Desiree was highest, indicating that maximum yield (22 kg) was produced by this variety.Keywords: Germplasm, potato, Solanum tuberosum L. Rawalakot, Azad KashmirAfrican Journal of Biotechnology Vol. 12(21), pp. 3214-322

Valvulogenesis of a living, innervated pulmonary root induced by an acellular scaffold

Heart valve disease is a major cause of mortality and morbidity worldwide with no effective medical therapy and no ideal valve substitute emulating the extremely sophisticated functions of a living heart valve. These functions influence survival and quality of life. This has stimulated extensive attempts at tissue engineering “living” heart valves. These attempts utilised combinations of allogeneic/ autologous cells and biological scaffolds with practical, regulatory, and ethical issues. In situ regeneration depends on scaffolds that attract, house and instruct cells and promote connective tissue formation. We describe a surgical, tissue-engineered, anatomically precise, novel off-the-shelf, acellular, synthetic scaffold inducing a rapid process of morphogenesis involving relevant cell types, extracellular matrix, regulatory elements including nerves and humoral components. This process relies on specific material characteristics, design and “morphodynamism”.</p

Profiling Genome-Wide DNA Methylation Patterns in Human Aortic and Mitral Valves

Cardiac valves exhibit highly complex structures and specialized functions that include dynamic interactions between cells, extracellular matrix (ECM) and their hemodynamic environment. Valvular gene expression is tightly regulated by a variety of mechanisms including epigenetic factors such as histone modifications, RNA-based mechanisms and DNA methylation. To date, methylation fingerprints of non-diseased human aortic and mitral valves have not been studied. In this work we analyzed the differential methylation profiles of 12 non-diseased aortic and mitral valve tissue samples (in matched pairs). Analysis of methylation data [reduced representation bisulfite sequencing (RRBS)] of 16,101 promoters genome-wide revealed 584 differentially methylated (DM) promoters, of which 13 were reported in endothelial mesenchymal trans-differentiation (EMT), 37 in aortic and mitral valve disease and 7 in ECM remodeling. Both functional classification as well as network analysis showed that the genes associated with the DM promoters were enriched for WNT-, Cadherin-, Endothelin-, PDGF-, HIF-1 and VEGF- signaling implicated in valvular physiology and pathophysiology. Additional enrichment was detected for TGFB-, NOTCH- and Integrin- signaling involved in EMT as well as ECM remodeling. This data provides the first insight into differential regulation of human aortic and mitral valve tissue and identifies candidate genes linked to DM promoters. Our work will improve the understanding of valve biology, valve tissue engineering approaches and contributes to the identification of relevant drug targets

The potential of anisotropic matrices as substrate for heart valve engineering

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CHALLENGES FACED BY RURAL WOMEN IN DATES PROCESSING INDUSTRY IN KHAIRPUR MIRS

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Cell abundance and diversity of phytoplankton in coastal water of Tioman Island

Study on the abundance and diversity has been carried out in Tioman Island from 27th to 30th August 2012. Integrated samples were collected at 16 stations around Tioman coastal waters. Results showed that 116 species of phytoplankton were identified and diatom was the dominant genus followed by dinoflagellates. Potential harmful algal bloom (HAB) species identified were Alexandrium sp, P. sigmoides, Dinophysis caudata, Ceratium furca, C. fusus, C. tripos, Noctiluca scintillans, Chaetoceros spp and Skeletonema costatum. The highest abundance of phytoplankton was at station 14 with cell density of 7.8 x 103 cells/L. The value of diversity index (H) and number of phytoplankton taxa for this station were 3.05 and 0.0.49, respectively. Results indicate that Tioman coastal water has high diversity of phytoplankton

Heat Stress Contributes to the Enhancement of Cardiac Mitochondrial Complex Activity

Hyperthermic stress is known to protect against myocardial dysfunction after ischemia-reperfusion injury. It is unclear however, what energetic mechanisms are affected by the molecular adaptation to heat stress. We hypothesized that mild hyperthermic stress can increase mitochondrial respiratory enzyme activity, affording protection to mitochondrial energetics during prolonged cardiac preservation for transplantation. Rat hearts were excised after heat-stress or sham treatment and subjected to cold cardioplegic arrest and ischemia followed by reperfusion in an ex vivo perfusion system. Cardiac function, mitochondrial respiratory, and complex activities were assessed before and after ischemia. Heat shock protein (Hsp 32, 60, and 72) expression was increased in heat-stressed hearts. This was associated with increased mitochondrial complex activities in heat-stress versus sham-treated groups for complex I-V. During reperfusion, higher complex activities and respiratory control ratios were observed in heat-stressed versus sham-treated groups. Recovery of ventricular function was improved in heat-stressed hearts. Furthermore, mitochondria in reperfused heat-stressed myocardium exhibited intact membranes with packed, parallel, lamellar cristae, whereas in sham-treated myocardium, mitochondria were severely disrupted. This study provides the first evidence of heat-stress-mediated enhancement of mitochondrial energetic capacity. This is associated with increased tolerance to ischemia-reperfusion injury. Protection by heat stress against myocardial dysfunction may be partially due to enhancement of mitochondrial energetics