Three small transiting planets around the M dwarf host star LP 358-499

We report on the detection of three transiting small planets around the low-mass star LP 358-499 (K2-133), using photometric data from the Kepler-K2 mission. Using multiband photometry, we determine the host star to be an early M dwarf with an age likely older than a Gigayear. The three detected planets K2-133 b, c, and d have orbital periods of ca. 3, 4.9 and 11 days and transit depths of ca. 700, 1000 and 2000 ppm, respectively. We also report a planetary candidate in the system (EPIC 247887989.01) with a period of 26.6 days and a depth of ca. 1000 ppm, which may be at the inner edge of the stellar habitable zone, depending on the specific host star properties. Using the transit parameters and the stellar properties, we estimate that the innermost planet may be rocky. The system is suited for follow-up observations to measure planetary masses and JWST transmission spectra of planetary atmospheres.Comment: Accepted for publication in MNRAS Letters. Replaced previous arXiv version with final submitted versio

論説

“Ca. Homeothermaceae” population genome properties. (DOCX 19 kb

MOESM1 of Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast

Additional file 1: Table S1. Primers and PCR fragments amplified/used in this work. PXXX, promoter of gene XXX; T XXX , terminator of gene XXX; Y-GDNA, CEN.PK113-7D genomic DNA; sequence annealing to template in primers is shown in red and italics; over-lap sequence for over-lap extension PCR and Gibson Assembly is underlined; restriction sites used in cloning are shown in bold. Table S2. Molecular construction of plasmids used in this work. Figure S1. Plasmid rearrangement in the strain NC1D. Plasmid pPMVAd36 and [TRP1] from NC1D were digested by restriction enzymes NotI, SalI, SphI, BamHI and SbfI and gel figure was shown as the right-bottom figure. Figure S2. The growth profile of strain GH4 [CEN.PK113-5D derivative; ura3(1, 704 )::KlURA3] (1) on sucrose. The cells were pre-cultured on 40 g L-1 glucose. Mean values from duplicate experiments are shown. Figure S3. Logic charts for fed-batch feeding scripts: (a) carbon-source-restricted/DO-triggered fed-batch cultivation; (b) carbon-source-overflowed/carbon-source-pulsing fed-batch cultivation. Fs, feeding flow storage value; DOt, dissolved oxygen on-line value at time t; DOL, lowest dissolved oxygen storage value; T1, storage time; t, on-line time; Îź, specific rate of feeding flow increasement; N, agitatation speed; Nmax, the maxium agitation speed; FVs, feeding volume storage value; FVt, feeding volume on-line value; Vt, culture volume on-line value. Figure S4. Growth (OD600) and process values (Dissovled oxygen, DO; oxygen transfer rate, OTR; carbon transfer rate; CTR; respiration quotient, RQ) in fed-batch cultivation for strain N391DA, with feeding logics in Fig. S1a employed. (a&b), 600 g L-1 glucose feeding; (c&d), 600 g L-1 sucrose feeding; (e&f), 400 g L-1 glucose and 158 g L-1 ethanol feeding. Figure S5. Growth (OD600) and process values (Dissovled oxygen, DO; oxygen transfer rate, OTR; carbon transfer rate; CTR; respiration quotient, RQ) in fed-batch cultivation for strain N391DA, with feeding logics in Fig. S1b employed. (a&b), 600 g L-1 glucose feeding with 10 g L-1 glucose pulse; (c&d), 600 g L-1 glucose feeding with 20 g L-1 glucose pulse; (e&f), 600 g L-1 sucrose feeding with 20 g L-1 sucrose pulse. Figure S6. The influence of nerolidol on yeast growth. Synthetic minimal medium was used, which contained 6.7 g L-1 yeast nitrogen base (Sigma-Aldrich #Y0626; pH 6.0) and 20 g L-1 glucose. Isomer-mixed nerolidol (Sigma-Aldrich #H59605) was used. Tween 80 was added to homogenize nerolidol into liquid medium. Mean values Âą standard deviations are shown (N = 3)

Additional file 1: of Controlling heterologous gene expression in yeast cell factories on different carbon substrates and across the diauxic shift: a comparison of yeast promoter activities

Table S1. Fluorescence of the destabilized GFP (yEGFP-CLN2 PEST) in the strains cultivated on 2% v/v ethanol to OD600 = 0.90 ± 0.03. Table S2. Effect of difference glucose concentrations (linear regression) and different carbon sources (one-way ANOVA) on GFP fluorescence driven by various promoters. Table S3. The primers, the plasmids and the strains used in this work. Figure S1. Pre-evaluation of mid-log phase for microplate cultivation. Figure S2. Correlation of GFP fluorescence determination in the strains using either the destabilized GFP (yEGFP-CLN2 PEST) or the normal GFP (yEGFP) as the reporter. Figure S3. Post-hoc test for fluorescence levels (sorted from low to high) of various promoter-yEGFP strains on different carbon source. Figure S4. Yeast cultures with/without copper addition. Figure S5. De-repression of ADH2 promoter

MOESM3 of Systems analysis of methylerythritol-phosphate pathway flux in E. coli: insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite

Additional file 3. SRM proteomics data. Contains Skyline output: raw and normalized data for transition, peptide and protein counts

Lippia nodiflora Michx.

原著和名: イハダレサウ科名: クマツヅラ科 = Verbenaceae採集地: 千葉県 銚子市 黒生町 (下総 銚子市 黒生)採集日: 1987/8/16採集者: 萩庭丈壽整理番号: JH037642国立科学博物館整理番号: TNS-VS-98764

Effect of blocking complex I and II on human embryonic stem cell growth.

<p>Effect of blocking complex I and complex II on hESC growth at 20% and 2% oxygen. Plot shows the cell number per well 4 days after the addition of rotenone or D-α-tocopherol succinate (A-TOS) or to the cell culture medium block complex I and II respectively. Control has no drug added. Values are averages ± standard deviation, n = 3. * indicates a p-value <0.5, deemed statistically significant.</p

Growth profile of human embryonic stem cells cultured at physiological and atmospheric oxygen concentrations.

<p>hESCs were harvested as a single cell suspension and then FACS sorted into 6-well plates to ensure a uniform seeding distribution across all wells. The cells were then cultured at physiological, 2%, and atmospheric, 20%, oxygen concentrations. At each time point cells were harvested and counted using flow cytometry techniques. A) The phase contrast images represent the spatial distribution of cells in the well and colony morphology at time equals 0, 47 and 70 hours after the experiment initialisation. The cell seeding methodologies employed allowed for consistent uniform seeding which translated to consistent initial cell numbers. Scale bar represents 500 µm. B) Growth profile of hESCs showing the lag and exponential growth phase on the semi-log plot to determine the specific growth rates. Values are means ± standard deviation, n = 6.</p

Activity of human embryonic stem cells metabolic pathways cultured at physiological and atmospheric oxygen concentrations.

<p>A visual representation of the flux through key metabolic pathways of hESCs cultured at both physiological and atmospheric oxygen concentrations. At both oxygen concentrations glycolysis is disconnected from the TCA cycle. The flux through the glycolysis pathways is greater at physiological oxygen concentrations while the uptake of glutamine and the flux through the respiratory chain is greater at atmospheric oxygen concentrations.</p

Pluripotent marker expression of human embryonic stem cells.

<p>Pluripotent marker expression of hESCs cultured under physiological, 2%, and atmospheric, 20%, oxygen concentrations were tested by FACS analysis after the completion of each experiment to ensure that the cells remained pluripotent throughout the 100 hour experiment. Representative FACS plots showing the shift in marker expression from the negative controls at the endpoint of an experiment are shown. All cell samples were found to be 92–99% positive for both TG30 and Oct-4 after each experiment at both 2% and 20% oxygen.</p