Additional file 1: of Controlling heterologous gene expression in
yeast cell factories on different carbon substrates and across the diauxic shift: a
comparison of yeast promoter activities
Table S1. Fluorescence of the destabilized GFP (yEGFP-CLN2 PEST) in the strains cultivated on 2% v/v ethanol to OD600 = 0.90 ± 0.03. Table S2. Effect of difference glucose concentrations (linear regression) and different carbon sources (one-way ANOVA) on GFP fluorescence driven by various promoters. Table S3. The primers, the plasmids and the strains used in this work. Figure S1. Pre-evaluation of mid-log phase for microplate cultivation. Figure S2. Correlation of GFP fluorescence determination in the strains using either the destabilized GFP (yEGFP-CLN2 PEST) or the normal GFP (yEGFP) as the reporter. Figure S3. Post-hoc test for fluorescence levels (sorted from low to high) of various promoter-yEGFP strains on different carbon source. Figure S4. Yeast cultures with/without copper addition. Figure S5. De-repression of ADH2 promoter
