Effects of a monocomponent protease from Bacillus licheniformis on broiler performance, digestibility, and carcass yield

Two experiments were carried out to evaluate the effects of protease addition to the diet of broilers at a higher level (1× or 2×) than the nutritional value proposed for the enzyme. The first experiment, 1280 day-old chicks (Cobb500®) were randomly allocated (randomized block design, 2×2+1 factorial arrangement), five treatments, eight replicates containing 32 birds/replicate. Treatments consisted: control diet without protease (CD); CD + 1× nutritional value of the enzyme (CDM1); CD + 2× nutritional value of the enzyme (CDM2); CDM1 + protease; and CDM2 + protease. The experimental period was 42 days. The mean weight (AFW), feed intake (FI), weight gain (WG), feed conversion, and carcass yield were evaluated. Significant differences were observed for AFW, WG, FI, abdominal fat yield, and feet percentage in the carcass. In the second experiment, 120 Cobb500® chicks at 14 days of age were allotted in a completely randomized design, 2×2+1 factorial arrangement, five treatments, six replicates with four birds/replicate. The treatments were consistent with the first experiment. Significant improvements in the nitrogen balance were observed for the broilers that received protease. The use of the enzyme tested is recommended with the recommended nutritional matrix, improving the zootechnical indices of broilers

Protease monocomponent of Bacillus licheniformis in broiler diets

Submitted by Liliane Ferreira ([email protected]) on 2018-04-17T11:02:13Z No. of bitstreams: 2 Dissertação - Larissa Paula Silva Gomides - 2018.pdf: 1989079 bytes, checksum: 3bf945430c1e7aa48082c34c2ae9d96c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira ([email protected]) on 2018-04-18T11:05:47Z (GMT) No. of bitstreams: 2 Dissertação - Larissa Paula Silva Gomides - 2018.pdf: 1989079 bytes, checksum: 3bf945430c1e7aa48082c34c2ae9d96c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-04-18T11:05:47Z (GMT). No. of bitstreams: 2 Dissertação - Larissa Paula Silva Gomides - 2018.pdf: 1989079 bytes, checksum: 3bf945430c1e7aa48082c34c2ae9d96c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-03-28Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESTwo experiments were carried out to evaluate the effect of the addition of 0.02% protease enzyme (Ronozyme ProAct) on diets based on corn and soybean meal for broilers. In both experiments, the same treatments used: T1- control ration, T2- a value of the nutritional matrix proposed by the enzyme but without the additive, T3- twice the value proposed in T2 without enzyme, T4- T2 with enzyme, T5- T3 with enzyme. In the first experiment, to determine the performance and yield of carcass and cuts were housed 1200 broiler chicks of 1 day of age, distributed in a completely randomized design, totaling 5 treatments, with 8 replicates and 30 birds per box. In the second experiment for the determination of metabolizable coefficients and intestinal histomorphometry, the method of total collection of excreta and intestinal tract collection was used in 300 chicks housed in galvanized steel cages at 1 day of age, distributed in a completely randomized design , submitted to 5 treatments and 6 replicates of 10 birds each. In both experiments, data analysis was perform using analysis of variance (ANOVA). In the first experiment, when the data were statistically different at 10% probability, they were compared by the Scott Knott Test and in the second experiment, when the data were statistically different at 5%, they were compared by the Scott Knott Test. In the second experiment we also performed a multivariate analysis of the main components and estimated the Pearson correlations. Chickens fed the one valued with enzyme treatment obtained the same final weight and weight gain as the control until 35 days of age.The birds that received the control treatment presented higher carcass yield, and in the weight of the cuts, there were no statistical differences between the protease treatments. Diets with protease showed higher dry matter metabolizable coefficient and nitrogen metabolizable coefficient, demonstrating that enzyme supplementation was effective. There was higher villus height and villus ratio: ileus crypt in broilers fed diets supplemented with protease at 7 days of age, however, at 21 days protease inclusion was not superior to the control treatment. A strong positive and significant correlation was observe between metabolizable and intestinal histomorphometry variables. Therefore, recommended to use protease in broiler diets.Foram realizados dois experimentos com o objetivo de avaliar o efeito da adição da enzima protease 0,02% (Ronozyme ProAct) em dietas valorizadas a base de milho e farelo de soja para frangos de corte. Em ambos os experimentos foram utilizados os mesmos tratamentos: T1- ração controle, T2- uma valorização da matriz nutricional proposta pela enzima mas sem o aditivo, T3- o dobro da valorização proposta em T2 sem enzima, T4- T2 com enzima, T5- T3 com enzima. No primeiro experimento, para a determinação do desempenho e rendimento de carcaça e cortes, foram alojados 1200 pintos de corte de 1 dia de idade, distribuídos em delineamento inteiramente casualizado, totalizando 5 tratamentos, com 8 repetições e 30 aves por box. No segundo experimento para a determinação dos coeficientes de metabolizabilidade e histomorfometria intestinal, foi utilizado o método de coleta total de excretas e coleta dos seguimentos intestinais em 300 pintos de corte alojados em gaiolas de aço galvanizado com 1 dia de idade, distribuídos em delineamento inteiramente casualizado, submetidos à 5 tratamentos e 6 repetições de 10 aves cada. Em ambos experimentos, a análise de dados foi realizada utilizando-se a análise de variância (ANOVA). No primeiro experimento, quando os dados foram estatisticamente diferentes a 10% de probabilidade, foram comparados pelo Teste de Scott Knott e no segundo experimento, quando os dados foram estatisticamente diferentes a 5% foram comparados pelo Teste de Scott Knott. No segundo experimento também foi realizada análise multivariada de componentes principais e estimadas as correlações de Pearson. Frangos alimentados com o tratamento de uma valorização e inclusão da protease obtiveram o mesmo peso final e ganho de peso do tratamento controle até os 35 dias de idade. As aves que receberam o tratamento controle apresentaram maior rendimento de carcaça, e no peso dos cortes, não houve diferenças estatísticas entre os tratamentos com protease. As dietas que continham protease apresentaram maior coeficiente de metabolizabilidade da matéria seca (CMMS) e coeficiente de metabolizabilidade do nitrogênio (CMN), demonstrando que a suplementação da enzima foi eficaz. Houve maior altura de vilo e relação vilo:cripta do íleo em frangos alimentados com dietas suplementadas com protease aos 7 dias de idade, entretanto, aos 21 dias a inclusão da protease não foi superior ao tratamento controle. Foi observado forte correlação positiva e significativa entre as variáveis de metabolizabilidade e histomorfometria intestinal. Portanto, recomenda-se a utilização da protease em dietas para frangos de corte

Projeto passarinhando: Ampliando horizontes através da fotografia

Devido à rica diversidade de espécies de aves encontradas no Brasil e sua importância ecológica, é relevante que a sociedade conheça esses animais, contribuindo para a sua preservação e dos ambientes onde vivem. O objetivo do trabalho foi, a partir de fotografias de aves, realizar ações a fim de conscientizar a população e em especial estudantes do ensino básico e superior da rede pública de ensino de Divinópolis – MG, sobre a diversidade da avifauna do campus da Universidade do Estado de Minas Gerais (UEMG), Unidade Divinópolis, sua importância e preservação. As ações do projeto foram compostas por palestras em duas escolas públicas e na UEMG, exposições fotográficas das aves registradas no campus da UEMG – Unidade Divinópolis em duas escolas públicas e na própria universidade, e minicurso “Iniciação à Observação e Fotografia de Aves”, realizado na UEMG. As atividades realizadas nas escolas possibilitaram a participação de 174 estudantes do Ensino Fundamental II e Ensino Médio, além da comunidade escolar. Na universidade, a exposição fotográfica contou com um público estimado de 900 pessoas. Junto à exposição houve uma palestra e o lançamento do E-book: “Guia de Aves do campus da UEMG em Divinópolis – MG”. O minicurso teve carga horária de 8 horas, com aulas teóricas e práticas, e contou com 11 participantes. O projeto atingiu um público de diversas faixas etárias e níveis de escolaridade, propiciando novos conhecimentos e despertando interesse científico nos participantes. Palavras-chave: Aves; Educação ambiental; Conscientização Passarinhando project: expanding horizons through photography   Abstract: Due to the rich diversity of bird species found in Brazil and their ecological importance, it is relevant that society knows about those animals, contributing to their preservation and the environment where they live. The objective of this work was, from bird photographs, to carry out actions in order to raise public awareness – especially students of primary and higher education of Divinópolis public schools – about the diversity of the avifauna of UEMG campus in Divinópolis (University of the State of Minas Gerais, Brazil), its importance and preservation. The project's actions consisted of lectures at two public schools and in the UEMG; photographic exhibitions of birds registered around the UEMG campus in Divinópolis displayed at two public schools and at the university itself; and a short course named "Initiation to Bird Observation and Photography," held at UEMG. The school activities enabled the participation of 174 students from Elementary School II and High School and the school community. At the university, the photographic exhibition had an estimated audience of 900 people. Along with the exhibition, a lecture and the E-book "Guide to Birds at the UEMG Campus Divinópolis" were launched. The short course had an 8-hour workload, with theoretical and practical classes and 11 participants. The project reached an audience of different age groups and education levels, providing new knowledge and arousing scientific interest in the participants. Keywords: Birds; Environmental education; Awarenes

Performance, nutrient digestibility, and intestinal histomorphometry of broilers fed diet supplemented with chondroitin and glucosamine sulfates

We aimed to evaluate the performance, nutrient digestibility, and intestinal histomorphometry of broilers fed diet supplemented with chondroitin sulfate and glucosamine sulfate. The experiment was carried out with 320 male broiler chicks distributed in a completely randomized design in a 2×2 factorial scheme (0 and 0.1% chondroitin sulfate and 0 and 0.3% glucosamine sulfate), with eight replications of 10 birds. Performance was evaluated at 7 and 21 days of age, nutrient digestibility of the diet was performed from 18 to 21 days of age, and small intestine histomorphometry was evaluated at 21 days of age. Broilers fed diet supplemented with 0.3% glucosamine sulfate showed high final weight and weight gain. A significant interaction was observed between sulfates for digestibility coefficients of nitrogen, mineral matter, and calcium. The use of 0.1% chondroitin sulfate without glucosamine sulfate resulted in a reduced digestibility of nitrogen but increased digestibility of total minerals and calcium. Diets without chondroitin sulfate with 0.3% glucosamine sulfate increased the digestibility coefficients of mineral matter and calcium. A significant interaction was found for jejunum villus height, which was higher in broilers fed diet supplemented with 0.3% glucosamine sulfate, regardless of the inclusion of chondroitin sulfate. Thus, supplementation with glucosamine sulfate in broiler diets contributes to high weight gain and villus height. Sulfates used in isolation promote high digestibility of mineral