17 research outputs found

    ATLAS detector and physics performance: Technical Design Report, 1

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    A Novel Express Method to Determine Activity of Antitumor Enzyme L-Lysine-α-Oxidase of Trichoderma harzianum Rifai F-180

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    A novel express method is developed to determine activity of antitumor enzyme L-lysine-α-oxidase obtained by culturing Trichoderma harzianum Rifai F-180 fungus. The carcinogenic reagent ortho-dianisidine-hydrochloride was replaced in the reaction medium with environmentally friendly reagents of the chromogenic mixture that included tetramethylbenzidine. This method improved precision and sensitivity of ELISA by 10 and 40 times, respectively. In addition, it could detect activity of L-lysine-α-oxidase not only in the producer strains with a pronounced activity of this enzyme, but also in the strains where this activity has not been previously determined. © 2020, Springer Science+Business Media, LLC, part of Springer Nature

    Новый экспресс-метод определения активности противоопухолевого фермента L-лизин-α-оксидазы из <i>Trichoderma harzianum</i> Rifai F-180

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    For the first time, a new express method for the determination of the antitumor activity of L-lysine-α-oxidase obtained by cultivating the fungus Trichoderma harzianum Rifai F-180. The carcinogenic reagent, ortho-dianisidine-hydrochloride, which was present in the reaction medium, was replaced by environmentally friendly reagents of the chromogenic mixture, which included tetramethylbenzidine (TMB). This method is more accurate in determining the enzyme and has a 40-fold increased sensitivity. In addition, it provides the ability to determine enzyme activity in producer strains not only with pronounced L-lysine-α-oxidase activity, but also in those where this activity has not been previously determined.Представлен новый экспресс-метод определения активности противоопухолевого фермента L-лизин-α-оксидазы, полученного при культивировании гриба Trichoderma harzianum Rifai F-180. Содержащийся в реакционной среде канцерогенный реактив орто-дианизидингидрохлорид был заменён на экологически чистые реагенты хромогенной смеси, в которую входил тетраметилбензидин. Метод повышает точность определения фермента в 10 раз, обладает повышенной в 40 раз чувствительностью и обеспечивает возможность определения активности фермента не только у штаммов-продуцентов с выраженной активностью L-лизин-a-оксидазы, но и с неопределённой активностью

    ATLAS: technical proposal for a general-purpose p p experiment at the large hadron collider at CERN

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    ATLAS

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